gamma proteobacterium IMCC2047

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F3KCK0|F3KCK0_9GAMM Peptidyl-prolyl cis-trans isomerase ppiD OS=gamma proteobacterium IMCC2047 OX=434085 GN=imdm_458 PE=4 SV=1
MM1 pKa = 7.47TSTAAVAYY9 pKa = 7.52EE10 pKa = 4.62AGDD13 pKa = 3.62ILIKK17 pKa = 10.84GGAANVAPNEE27 pKa = 4.38DD28 pKa = 3.91SSDD31 pKa = 3.69VTPLAGAQGAKK42 pKa = 9.88VDD44 pKa = 4.01NNTQLGLTLTYY55 pKa = 9.85MYY57 pKa = 9.49TDD59 pKa = 4.22KK60 pKa = 11.14IGIEE64 pKa = 4.13LLAATPFEE72 pKa = 4.42HH73 pKa = 6.51TVTATGAVIGGADD86 pKa = 3.31VADD89 pKa = 4.31IKK91 pKa = 10.93HH92 pKa = 6.46LPPTLTLNYY101 pKa = 10.33YY102 pKa = 10.69FGDD105 pKa = 4.04SNSKK109 pKa = 7.63VQPYY113 pKa = 9.71IGAGINYY120 pKa = 7.7TIFFDD125 pKa = 4.63EE126 pKa = 4.52SADD129 pKa = 3.78SQFEE133 pKa = 4.36AIAGNTDD140 pKa = 2.53VDD142 pKa = 5.78LDD144 pKa = 4.42DD145 pKa = 3.75SWGLAFHH152 pKa = 7.26AGIDD156 pKa = 3.65YY157 pKa = 10.81KK158 pKa = 11.56LNDD161 pKa = 3.76KK162 pKa = 10.24WSLNAGYY169 pKa = 9.15WYY171 pKa = 10.91LDD173 pKa = 3.12IDD175 pKa = 4.02TEE177 pKa = 4.44ATLKK181 pKa = 9.01TAGVGVQKK189 pKa = 10.72VDD191 pKa = 3.05VDD193 pKa = 3.84IDD195 pKa = 3.74PDD197 pKa = 3.66VYY199 pKa = 10.71MIGLSYY205 pKa = 11.08KK206 pKa = 10.26FF207 pKa = 3.73

Molecular weight: 22.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F3KFK7|F3KFK7_9GAMM Cysteine desulfurase IscS OS=gamma proteobacterium IMCC2047 OX=434085 GN=iscS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.49IKK11 pKa = 10.29RR12 pKa = 11.84ARR14 pKa = 11.84AHH16 pKa = 6.27GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84LVIKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.46GRR39 pKa = 11.84LRR41 pKa = 11.84LTAA44 pKa = 4.67

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2263

0

2263

509404

37

1252

225.1

24.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.226 ± 0.059

1.125 ± 0.02

5.633 ± 0.041

6.467 ± 0.053

3.934 ± 0.041

7.034 ± 0.047

2.321 ± 0.031

5.907 ± 0.04

4.895 ± 0.045

10.682 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.656 ± 0.028

3.847 ± 0.033

4.144 ± 0.035

4.691 ± 0.05

5.292 ± 0.042

6.219 ± 0.039

4.995 ± 0.037

7.013 ± 0.049

1.19 ± 0.023

2.729 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski