Suillus luteus UH-Slu-Lm8-n1
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18277 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D0AIW7|A0A0D0AIW7_9AGAM Unplaced genomic scaffold CY34scaffold_1408 whole genome shotgun sequence OS=Suillus luteus UH-Slu-Lm8-n1 OX=930992 GN=CY34DRAFT_814464 PE=4 SV=1
LL1 pKa = 7.35 EE2 pKa = 4.16 GHH4 pKa = 6.28 TDD6 pKa = 3.2 SVYY9 pKa = 11.4 SLTLFQNDD17 pKa = 3.39 RR18 pKa = 11.84 LLASTSVDD26 pKa = 3.11 DD27 pKa = 5.01 TARR30 pKa = 11.84 LWDD33 pKa = 4.64 LDD35 pKa = 3.77 TNLQVGPPLQHH46 pKa = 6.22 EE47 pKa = 4.64 NEE49 pKa = 4.34 VEE51 pKa = 4.12 CAAFSTDD58 pKa = 3.4 GKK60 pKa = 10.78 LLSTACDD67 pKa = 3.73 DD68 pKa = 3.71 NNAYY72 pKa = 9.87 VWDD75 pKa = 4.36 IYY77 pKa = 11.72
Molecular weight: 8.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.541
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.884
Patrickios 0.769
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.803
Protein with the highest isoelectric point:
>tr|A0A0D0ABS6|A0A0D0ABS6_9AGAM Unplaced genomic scaffold CY34scaffold_221 whole genome shotgun sequence OS=Suillus luteus UH-Slu-Lm8-n1 OX=930992 GN=CY34DRAFT_89578 PE=4 SV=1
MM1 pKa = 7.33 GAFAFFGPGKK11 pKa = 10.14 FGNLHH16 pKa = 6.14 TSLSNPVAGRR26 pKa = 11.84 GSIRR30 pKa = 11.84 QRR32 pKa = 11.84 MASRR36 pKa = 11.84 PRR38 pKa = 11.84 NARR41 pKa = 11.84 IPRR44 pKa = 11.84 FPILLRR50 pKa = 11.84 KK51 pKa = 9.14 VLHH54 pKa = 6.5 KK55 pKa = 10.68 LGSQPRR61 pKa = 11.84 VGG63 pKa = 3.72
Molecular weight: 6.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.14
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.544
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.281
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.147
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18271
6
18277
5969091
49
5054
326.6
36.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.102 ± 0.02
1.545 ± 0.008
5.6 ± 0.016
5.538 ± 0.019
3.827 ± 0.012
6.047 ± 0.017
2.873 ± 0.011
5.201 ± 0.015
4.413 ± 0.016
9.409 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.225 ± 0.008
3.49 ± 0.01
6.15 ± 0.022
3.829 ± 0.014
6.214 ± 0.015
8.934 ± 0.026
6.087 ± 0.014
6.336 ± 0.014
1.522 ± 0.008
2.656 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here