Pneumovirus dog/Bari/100-12/ITA/2012

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus; Murine orthopneumovirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0ISD5|W0ISD5_9MONO Protein M2-1 OS=Pneumovirus dog/Bari/100-12/ITA/2012 OX=2482952 GN=M2 PE=3 SV=1
MM1 pKa = 7.59EE2 pKa = 4.81KK3 pKa = 10.16FAPEE7 pKa = 4.1FVGEE11 pKa = 4.24DD12 pKa = 3.32ANKK15 pKa = 10.21KK16 pKa = 10.09AEE18 pKa = 4.2EE19 pKa = 4.03FLKK22 pKa = 10.78HH23 pKa = 6.57RR24 pKa = 11.84SFPSEE29 pKa = 4.14KK30 pKa = 9.72PLAGIPNTATHH41 pKa = 4.6VTKK44 pKa = 11.22YY45 pKa = 10.91NMPPILRR52 pKa = 11.84SSFKK56 pKa = 10.63LSPPRR61 pKa = 11.84AAAKK65 pKa = 8.23LTKK68 pKa = 9.98PSTPPSTPPPTPPQNKK84 pKa = 9.02EE85 pKa = 4.0EE86 pKa = 4.07QPKK89 pKa = 10.73EE90 pKa = 3.77SDD92 pKa = 3.12ADD94 pKa = 3.27IEE96 pKa = 4.56TIHH99 pKa = 7.75VYY101 pKa = 9.44EE102 pKa = 4.64VPDD105 pKa = 3.74NPEE108 pKa = 3.82HH109 pKa = 6.97SKK111 pKa = 10.94KK112 pKa = 10.2PCCSDD117 pKa = 3.33DD118 pKa = 3.54TDD120 pKa = 3.73TKK122 pKa = 10.05KK123 pKa = 8.88TRR125 pKa = 11.84KK126 pKa = 9.57PMVTFVEE133 pKa = 4.45PEE135 pKa = 3.94EE136 pKa = 4.52KK137 pKa = 10.32FVGLGASLYY146 pKa = 10.34KK147 pKa = 10.05EE148 pKa = 4.45TMQTFAADD156 pKa = 4.28GYY158 pKa = 10.96DD159 pKa = 3.57EE160 pKa = 4.49EE161 pKa = 5.77SNLSFEE167 pKa = 4.68EE168 pKa = 4.5TNQEE172 pKa = 4.04PGSSSVEE179 pKa = 3.72QRR181 pKa = 11.84LDD183 pKa = 3.74RR184 pKa = 11.84IEE186 pKa = 4.7EE187 pKa = 4.01KK188 pKa = 10.6LSYY191 pKa = 10.39IIGLLNTIMVATAGPTTARR210 pKa = 11.84DD211 pKa = 3.91EE212 pKa = 4.32IRR214 pKa = 11.84DD215 pKa = 3.73ALIGTRR221 pKa = 11.84EE222 pKa = 4.02EE223 pKa = 4.52LIEE226 pKa = 4.22MIKK229 pKa = 10.57SDD231 pKa = 3.56ILTVNDD237 pKa = 5.27RR238 pKa = 11.84IAAMEE243 pKa = 4.0KK244 pKa = 10.54LRR246 pKa = 11.84DD247 pKa = 3.97EE248 pKa = 4.57EE249 pKa = 4.34CSRR252 pKa = 11.84AEE254 pKa = 3.96TDD256 pKa = 5.15DD257 pKa = 5.08GSACYY262 pKa = 8.95LTDD265 pKa = 3.61RR266 pKa = 11.84ARR268 pKa = 11.84ILDD271 pKa = 4.28KK272 pKa = 10.67IVSSNAEE279 pKa = 3.88EE280 pKa = 4.12AKK282 pKa = 10.64EE283 pKa = 4.12DD284 pKa = 4.47LDD286 pKa = 3.84VDD288 pKa = 4.74DD289 pKa = 5.61IMGINFF295 pKa = 3.75

Molecular weight: 32.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0ISH6|W0ISH6_9MONO Non-structural protein 2 OS=Pneumovirus dog/Bari/100-12/ITA/2012 OX=2482952 GN=NS2 PE=4 SV=1
MM1 pKa = 7.5SVRR4 pKa = 11.84PCKK7 pKa = 10.54FEE9 pKa = 3.87VQGFCSRR16 pKa = 11.84GRR18 pKa = 11.84NCKK21 pKa = 9.77YY22 pKa = 9.6SHH24 pKa = 7.19KK25 pKa = 9.69YY26 pKa = 8.64WEE28 pKa = 4.56WPLKK32 pKa = 10.13TLMLRR37 pKa = 11.84QNYY40 pKa = 7.16MLNRR44 pKa = 11.84IYY46 pKa = 10.91RR47 pKa = 11.84FLDD50 pKa = 3.34TNTDD54 pKa = 3.0AMSDD58 pKa = 3.47VSGFDD63 pKa = 3.07APQRR67 pKa = 11.84TAEE70 pKa = 4.23YY71 pKa = 10.2ALGTIGVLKK80 pKa = 10.67SYY82 pKa = 10.88LEE84 pKa = 3.97KK85 pKa = 10.55TNNITKK91 pKa = 10.45SIACGSLITVLQNLDD106 pKa = 3.37VGLVIQARR114 pKa = 11.84DD115 pKa = 3.5SNAEE119 pKa = 3.72DD120 pKa = 3.37TNYY123 pKa = 10.27LRR125 pKa = 11.84SCNTILSYY133 pKa = 10.32IDD135 pKa = 4.64KK136 pKa = 9.89IHH138 pKa = 7.44KK139 pKa = 9.03KK140 pKa = 9.61RR141 pKa = 11.84QVIHH145 pKa = 6.62ILKK148 pKa = 9.24KK149 pKa = 10.24LPVGVLCSLIQSVISIEE166 pKa = 4.08EE167 pKa = 4.38KK168 pKa = 10.2INSSMKK174 pKa = 10.28TEE176 pKa = 3.83

Molecular weight: 20.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

1

11

4571

92

2040

415.5

46.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.294 ± 1.031

2.231 ± 0.4

5.207 ± 0.427

5.163 ± 0.663

3.566 ± 0.319

4.9 ± 0.468

2.406 ± 0.311

6.913 ± 0.604

6.344 ± 0.47

10.785 ± 1.103

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.174

5.907 ± 0.375

4.31 ± 0.805

3.128 ± 0.315

4.813 ± 0.231

8.335 ± 0.424

6.607 ± 0.758

6.454 ± 0.462

1.116 ± 0.236

3.807 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski