Petrimonas mucosa
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2896 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G4G9B4|A0A1G4G9B4_9BACT N-glycanase_N domain-containing protein OS=Petrimonas mucosa OX=1642646 GN=ING2E5A_2344 PE=4 SV=1
MM1 pKa = 7.82 KK2 pKa = 9.36 ITDD5 pKa = 3.74 SKK7 pKa = 11.42 YY8 pKa = 9.03 VTLTYY13 pKa = 10.76 DD14 pKa = 3.63 LNVGEE19 pKa = 4.25 GDD21 pKa = 3.32 EE22 pKa = 4.46 RR23 pKa = 11.84 EE24 pKa = 4.44 LMEE27 pKa = 4.0 QATPEE32 pKa = 4.1 RR33 pKa = 11.84 PLEE36 pKa = 4.37 FIFGTNSMLKK46 pKa = 10.27 AFEE49 pKa = 4.6 DD50 pKa = 3.83 NIYY53 pKa = 10.7 GLSEE57 pKa = 3.94 GDD59 pKa = 3.48 SFKK62 pKa = 11.08 FSLTPDD68 pKa = 3.51 EE69 pKa = 5.54 AYY71 pKa = 10.71 GDD73 pKa = 3.83 YY74 pKa = 11.19 DD75 pKa = 3.42 EE76 pKa = 5.25 TRR78 pKa = 11.84 IIDD81 pKa = 3.69 LPKK84 pKa = 10.69 SIFEE88 pKa = 3.96 VDD90 pKa = 3.23 GKK92 pKa = 10.39 IDD94 pKa = 3.46 HH95 pKa = 6.57 QMLFEE100 pKa = 5.11 GNTLPMMDD108 pKa = 3.56 SDD110 pKa = 4.41 GNRR113 pKa = 11.84 LTGSVVSITEE123 pKa = 4.01 DD124 pKa = 3.35 TVTMDD129 pKa = 4.49 FNHH132 pKa = 7.02 PLAGEE137 pKa = 4.18 TMHH140 pKa = 6.96 FEE142 pKa = 4.1 GVIQGVRR149 pKa = 11.84 DD150 pKa = 3.8 ASPEE154 pKa = 3.8 EE155 pKa = 3.82 IAALFSGGGCGCGNDD170 pKa = 3.43 SCGCGDD176 pKa = 4.09 EE177 pKa = 5.44 GDD179 pKa = 4.05 SCGCGSGDD187 pKa = 3.6 GGCGGGCSCC196 pKa = 6.0
Molecular weight: 20.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.668
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.579
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.986
Patrickios 0.871
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A1G4G968|A0A1G4G968_9BACT Alpha-L-rhamnosidase OS=Petrimonas mucosa OX=1642646 GN=ING2E5A_2273 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 KK11 pKa = 9.07 RR12 pKa = 11.84 KK13 pKa = 8.85 NKK15 pKa = 10.06 HH16 pKa = 4.93 GFLSRR21 pKa = 11.84 MATKK25 pKa = 10.55 NGRR28 pKa = 11.84 KK29 pKa = 9.16 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.17 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSDD46 pKa = 3.89
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.381
IPC2_protein 10.818
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.048
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2896
0
2896
1089703
29
2304
376.3
42.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.739 ± 0.035
0.949 ± 0.014
5.414 ± 0.03
6.848 ± 0.043
4.727 ± 0.03
7.032 ± 0.036
1.916 ± 0.019
6.98 ± 0.039
6.126 ± 0.04
9.292 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.506 ± 0.017
5.06 ± 0.036
3.981 ± 0.025
3.291 ± 0.023
5.433 ± 0.035
6.383 ± 0.033
5.327 ± 0.03
6.493 ± 0.034
1.324 ± 0.019
4.182 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here