Kaistia soli DSM 19436
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4866 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5IBL2|A0A1M5IBL2_9RHIZ Simple sugar transport system permease protein OS=Kaistia soli DSM 19436 OX=1122133 GN=SAMN02745157_3831 PE=4 SV=1
MM1 pKa = 7.17 SQFPASQSGWLPARR15 pKa = 11.84 GSALLASASVLALTLGFVPDD35 pKa = 3.74 RR36 pKa = 11.84 VHH38 pKa = 6.44 AAGWNLYY45 pKa = 10.32 DD46 pKa = 5.82 GYY48 pKa = 8.16 TQSYY52 pKa = 8.89 SVTYY56 pKa = 10.75 ANSLEE61 pKa = 4.1 TALADD66 pKa = 4.32 KK67 pKa = 9.32 DD68 pKa = 4.05 TLHH71 pKa = 6.69 VGLSVGGSPVTVTMDD86 pKa = 3.2 TGSVGLVLSANHH98 pKa = 5.75 VASYY102 pKa = 8.59 STSGTPGWEE111 pKa = 4.39 YY112 pKa = 11.28 YY113 pKa = 10.49 NSSGLLLTGYY123 pKa = 10.55 FNDD126 pKa = 3.8 YY127 pKa = 9.19 TVEE130 pKa = 4.17 LDD132 pKa = 3.82 NGTDD136 pKa = 3.43 ANGNPTTVTATLPVLVVTEE155 pKa = 4.99 AYY157 pKa = 10.06 CLGVGSDD164 pKa = 3.59 PCDD167 pKa = 3.5 AEE169 pKa = 3.97 ASKK172 pKa = 11.35 NSVSMMGVGYY182 pKa = 10.45 DD183 pKa = 3.61 RR184 pKa = 11.84 NTMGTGSVDD193 pKa = 3.42 LSLSGKK199 pKa = 8.08 QLNEE203 pKa = 3.93 QLNAAPTTSEE213 pKa = 4.44 AYY215 pKa = 10.67 NLFLNIDD222 pKa = 4.07 GMAEE226 pKa = 3.72 GALRR230 pKa = 11.84 RR231 pKa = 11.84 GYY233 pKa = 10.11 IITPTGVEE241 pKa = 4.23 LGLTAANTSSQAFTYY256 pKa = 10.06 AQLVLNSAGTNGATSNWQSVAADD279 pKa = 3.44 VTLAGTSSTATLLMDD294 pKa = 3.77 TGITGSFFEE303 pKa = 4.79 IPGGTEE309 pKa = 3.98 GPATAGTVITISLAGGSATYY329 pKa = 10.74 SFVVGDD335 pKa = 3.74 TANPQTPGTVTIGPPAAAFVNSGLHH360 pKa = 5.64 TYY362 pKa = 10.94 AGFNVLFDD370 pKa = 4.24 ADD372 pKa = 4.02 GGFLGVAANGFSGATNASVTQLIAATGPLTLTQAFEE408 pKa = 4.06 TDD410 pKa = 4.06 LPVMLLDD417 pKa = 4.32 ASTINTSTTATFDD430 pKa = 3.17 AGIFGPGSLTLNGGTVVLNGAVTNGGGVTAASGTTALNGTMTGNLTVASGASFYY484 pKa = 11.27 NYY486 pKa = 10.07 NNGYY490 pKa = 10.07 AVAAGNILVNDD501 pKa = 4.25 GLFVGANSGAAFVNAGTVDD520 pKa = 3.34 NSGSFVGAVNNSGSWTNSGTLTGDD544 pKa = 3.35 VTNSGTFSNSNLVDD558 pKa = 3.8 GNITNTGSLTNTGEE572 pKa = 3.89 IEE574 pKa = 4.24 GDD576 pKa = 3.7 VTSTGPIANQGTVTGTLTVYY596 pKa = 10.1 NQHH599 pKa = 6.41 SGNGTVGTLSAKK611 pKa = 10.19 PGALVSPGNSVGTIIVSGDD630 pKa = 2.98 ATFEE634 pKa = 4.55 PGSVLYY640 pKa = 10.88 AEE642 pKa = 5.53 LGANGLSDD650 pKa = 4.17 LLVVGGTLVADD661 pKa = 4.67 GATLYY666 pKa = 10.68 LAAANGFEE674 pKa = 4.15 PVLGNSYY681 pKa = 11.14 SVIQAGSIASNFTVASPFFGSTASPFPFLGASLDD715 pKa = 3.82 GTGVLTLGRR724 pKa = 11.84 SALRR728 pKa = 11.84 FEE730 pKa = 5.26 DD731 pKa = 4.6 FAVTQNEE738 pKa = 3.89 RR739 pKa = 11.84 MAASAAEE746 pKa = 4.1 TLGLQSPLNQALALMSIAEE765 pKa = 4.3 VPSVFDD771 pKa = 3.66 SLSGEE776 pKa = 3.89 IAASAEE782 pKa = 4.23 STLQQQSIYY791 pKa = 10.71 LRR793 pKa = 11.84 DD794 pKa = 3.47 AVTGRR799 pKa = 11.84 VRR801 pKa = 11.84 QAFSDD806 pKa = 3.62 AAGPEE811 pKa = 4.08 ASGSQTARR819 pKa = 11.84 LAPGLDD825 pKa = 3.08 ATAWTQAYY833 pKa = 7.81 GAWGNSWSDD842 pKa = 3.2 GNAAAVSRR850 pKa = 11.84 SIGGFLLGADD860 pKa = 4.11 AALGDD865 pKa = 3.75 AWRR868 pKa = 11.84 VGLAGGYY875 pKa = 8.16 SQSDD879 pKa = 3.66 FSLDD883 pKa = 3.42 GVGGGGTSDD892 pKa = 3.67 NYY894 pKa = 11.34 DD895 pKa = 3.18 VAIYY899 pKa = 10.46 GGTRR903 pKa = 11.84 QGDD906 pKa = 3.04 ASLRR910 pKa = 11.84 FGAGYY915 pKa = 7.69 TWHH918 pKa = 7.88 DD919 pKa = 3.27 IATGRR924 pKa = 11.84 TALLPTTAEE933 pKa = 4.26 FLSADD938 pKa = 3.95 YY939 pKa = 10.89 QGGTAQVFGEE949 pKa = 3.96 AAYY952 pKa = 10.14 DD953 pKa = 3.63 VRR955 pKa = 11.84 LGRR958 pKa = 11.84 AVLEE962 pKa = 4.57 PYY964 pKa = 10.99 VNLAYY969 pKa = 11.05 VNLTMDD975 pKa = 4.65 GFWEE979 pKa = 4.33 TGGAAALTFAEE990 pKa = 4.67 STMSTTFNVLGMRR1003 pKa = 11.84 LGQAFDD1009 pKa = 3.67 IGNGLQLLTRR1019 pKa = 11.84 GSLGWQHH1026 pKa = 6.8 AFGDD1030 pKa = 3.88 ITPQATAAFLGSSAFTVAGLPIAQDD1055 pKa = 3.19 AALIDD1060 pKa = 3.72 AFIGFRR1066 pKa = 11.84 PTSRR1070 pKa = 11.84 VDD1072 pKa = 3.57 FGLRR1076 pKa = 11.84 YY1077 pKa = 9.89 SGQIADD1083 pKa = 4.64 DD1084 pKa = 3.82 ATDD1087 pKa = 3.46 NAVQGTLDD1095 pKa = 3.11 IRR1097 pKa = 11.84 FF1098 pKa = 3.63
Molecular weight: 110.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.872
IPC_protein 3.91
Toseland 3.681
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.732
Grimsley 3.579
Solomon 3.897
Lehninger 3.846
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.846
Sillero 4.024
Patrickios 0.782
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.904
Protein with the highest isoelectric point:
>tr|A0A1M5NXS0|A0A1M5NXS0_9RHIZ Uncharacterized protein OS=Kaistia soli DSM 19436 OX=1122133 GN=SAMN02745157_0039 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.43 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.38 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.36 VIAQRR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.47 KK41 pKa = 9.66 LTAA44 pKa = 4.17
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4866
0
4866
1558496
35
3023
320.3
34.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.309 ± 0.05
0.69 ± 0.011
5.77 ± 0.026
5.326 ± 0.034
3.821 ± 0.025
8.841 ± 0.042
1.951 ± 0.016
5.536 ± 0.022
2.898 ± 0.026
10.126 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.356 ± 0.015
2.398 ± 0.023
5.379 ± 0.027
2.722 ± 0.018
7.021 ± 0.04
5.454 ± 0.027
5.349 ± 0.027
7.595 ± 0.029
1.319 ± 0.015
2.137 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here