Sewage-associated circular DNA virus-23
Average proteome isoelectric point is 8.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UH82|A0A0B4UH82_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-23 OX=1592090 PE=3 SV=1
MM1 pKa = 7.71 TSRR4 pKa = 11.84 KK5 pKa = 7.75 WVFTVNNYY13 pKa = 9.04 TDD15 pKa = 5.17 DD16 pKa = 3.29 EE17 pKa = 4.48 WNEE20 pKa = 3.78 LKK22 pKa = 10.33 RR23 pKa = 11.84 WCMVNTKK30 pKa = 9.52 YY31 pKa = 10.99 AIMGKK36 pKa = 9.13 EE37 pKa = 3.98 VGPQCGTPHH46 pKa = 5.4 IQGYY50 pKa = 10.2 LSANKK55 pKa = 8.29 PHH57 pKa = 6.8 RR58 pKa = 11.84 RR59 pKa = 11.84 SGMVEE64 pKa = 3.5 VCRR67 pKa = 11.84 RR68 pKa = 11.84 ARR70 pKa = 11.84 WEE72 pKa = 3.74 AAKK75 pKa = 11.14 GNDD78 pKa = 3.7 TQNDD82 pKa = 4.2 DD83 pKa = 4.13 YY84 pKa = 11.32 CRR86 pKa = 11.84 KK87 pKa = 9.16 AAKK90 pKa = 9.72 EE91 pKa = 3.75 GRR93 pKa = 11.84 EE94 pKa = 4.27 VWRR97 pKa = 11.84 HH98 pKa = 4.63 GQPCTGAGQRR108 pKa = 11.84 TDD110 pKa = 3.79 LLRR113 pKa = 11.84 VAAAVDD119 pKa = 3.55 SGVSLRR125 pKa = 11.84 EE126 pKa = 3.75 IASEE130 pKa = 4.0 NPSEE134 pKa = 4.27 FIKK137 pKa = 9.92 FHH139 pKa = 6.63 RR140 pKa = 11.84 GILAYY145 pKa = 10.41 RR146 pKa = 11.84 NLVRR150 pKa = 11.84 PAVPRR155 pKa = 11.84 DD156 pKa = 3.78 FKK158 pKa = 11.11 TEE160 pKa = 3.51 LHH162 pKa = 6.19 VFVGPPGSGKK172 pKa = 8.75 SRR174 pKa = 11.84 RR175 pKa = 11.84 AQEE178 pKa = 4.03 LASSFGTVYY187 pKa = 10.57 RR188 pKa = 11.84 KK189 pKa = 10.03 VRR191 pKa = 11.84 GPWWNGYY198 pKa = 7.37 EE199 pKa = 4.06 QQQSVIIDD207 pKa = 3.88 DD208 pKa = 4.55 FYY210 pKa = 11.41 GWVPFDD216 pKa = 3.47 EE217 pKa = 4.9 LLRR220 pKa = 11.84 VADD223 pKa = 5.18 RR224 pKa = 11.84 YY225 pKa = 7.84 QHH227 pKa = 4.75 QVEE230 pKa = 4.22 IKK232 pKa = 10.67 GGFEE236 pKa = 4.01 EE237 pKa = 4.88 FNSKK241 pKa = 10.32 IIIITSNRR249 pKa = 11.84 PIKK252 pKa = 10.28 DD253 pKa = 2.71 WYY255 pKa = 10.85 KK256 pKa = 10.13 FDD258 pKa = 4.69 SYY260 pKa = 11.67 DD261 pKa = 3.38 PEE263 pKa = 4.8 ALYY266 pKa = 10.49 RR267 pKa = 11.84 RR268 pKa = 11.84 CSVYY272 pKa = 9.42 QHH274 pKa = 6.51 INMDD278 pKa = 3.65 HH279 pKa = 6.51 SVTDD283 pKa = 3.61 YY284 pKa = 11.47 EE285 pKa = 4.05 IMEE288 pKa = 4.94 GIKK291 pKa = 10.11 INYY294 pKa = 8.27
Molecular weight: 34.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.967
IPC2_protein 8.097
IPC_protein 8.024
Toseland 7.995
ProMoST 8.595
Dawson 8.726
Bjellqvist 8.902
Wikipedia 8.741
Rodwell 8.756
Grimsley 7.951
Solomon 8.858
Lehninger 8.829
Nozaki 8.99
DTASelect 8.697
Thurlkill 8.756
EMBOSS 8.887
Sillero 9.019
Patrickios 4.38
IPC_peptide 8.843
IPC2_peptide 7.805
IPC2.peptide.svr19 7.88
Protein with the highest isoelectric point:
>tr|A0A0B4UH82|A0A0B4UH82_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-23 OX=1592090 PE=3 SV=1
MM1 pKa = 7.39 ARR3 pKa = 11.84 YY4 pKa = 8.27 PKK6 pKa = 10.7 ASGNRR11 pKa = 11.84 YY12 pKa = 9.27 APYY15 pKa = 9.93 IRR17 pKa = 11.84 TAGRR21 pKa = 11.84 MYY23 pKa = 10.83 GAAKK27 pKa = 9.6 KK28 pKa = 10.53 AYY30 pKa = 9.45 GIYY33 pKa = 9.98 SKK35 pKa = 10.99 VRR37 pKa = 11.84 TRR39 pKa = 11.84 YY40 pKa = 9.07 RR41 pKa = 11.84 NSGSRR46 pKa = 11.84 TKK48 pKa = 10.49 QKK50 pKa = 10.4 RR51 pKa = 11.84 NYY53 pKa = 7.86 RR54 pKa = 11.84 KK55 pKa = 10.04 KK56 pKa = 10.12 YY57 pKa = 7.76 GGAMGSSIPGRR68 pKa = 11.84 CGIYY72 pKa = 9.64 VRR74 pKa = 11.84 KK75 pKa = 9.57 GRR77 pKa = 11.84 KK78 pKa = 8.99 DD79 pKa = 2.92 RR80 pKa = 11.84 WLKK83 pKa = 10.87 NLTKK87 pKa = 10.59 NSPPRR92 pKa = 11.84 LLLNQSTNQVEE103 pKa = 4.23 SDD105 pKa = 3.15 AGKK108 pKa = 10.38 QGTLSILQTLTRR120 pKa = 11.84 TDD122 pKa = 4.12 FMNMKK127 pKa = 8.87 ATLPAISDD135 pKa = 3.52 TGLGSGAAVGFATVSYY151 pKa = 11.19 YY152 pKa = 10.17 MDD154 pKa = 3.79 IALKK158 pKa = 10.19 RR159 pKa = 11.84 LKK161 pKa = 10.9 AEE163 pKa = 3.7 ILITNTCNSVVFVKK177 pKa = 10.07 MRR179 pKa = 11.84 TWLCKK184 pKa = 9.9 KK185 pKa = 10.26 SSNASPLEE193 pKa = 3.75 KK194 pKa = 9.42 WGEE197 pKa = 3.82 ILNVQNANVIPANKK211 pKa = 9.79 VLTTTYY217 pKa = 10.71 GATPYY222 pKa = 10.08 MVDD225 pKa = 5.39 GFNTWWKK232 pKa = 10.31 CVQTRR237 pKa = 11.84 NLRR240 pKa = 11.84 LAPGEE245 pKa = 4.3 IYY247 pKa = 10.61 CHH249 pKa = 5.05 NHH251 pKa = 5.24 IDD253 pKa = 4.23 KK254 pKa = 10.41 INKK257 pKa = 8.96 IIRR260 pKa = 11.84 GSSYY264 pKa = 9.93 EE265 pKa = 4.0 AEE267 pKa = 3.92 QNLYY271 pKa = 10.03 IRR273 pKa = 11.84 GLSKK277 pKa = 10.78 VVTITIHH284 pKa = 6.88 GEE286 pKa = 3.72 PTQDD290 pKa = 3.04 STGVNTLSGAVVNVAGKK307 pKa = 10.27 RR308 pKa = 11.84 KK309 pKa = 7.74 PTQIHH314 pKa = 5.77
Molecular weight: 34.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.285
IPC2_protein 9.809
IPC_protein 10.131
Toseland 10.409
ProMoST 10.058
Dawson 10.57
Bjellqvist 10.233
Wikipedia 10.73
Rodwell 11.038
Grimsley 10.628
Solomon 10.599
Lehninger 10.57
Nozaki 10.394
DTASelect 10.233
Thurlkill 10.423
EMBOSS 10.789
Sillero 10.482
Patrickios 10.687
IPC_peptide 10.599
IPC2_peptide 9.063
IPC2.peptide.svr19 8.626
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
608
294
314
304.0
34.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.908 ± 0.549
1.809 ± 0.162
4.112 ± 1.167
4.605 ± 1.535
2.467 ± 0.89
8.059 ± 0.402
1.974 ± 0.522
6.086 ± 0.212
7.237 ± 1.016
5.592 ± 1.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.303 ± 0.183
5.921 ± 0.81
4.112 ± 0.216
3.618 ± 0.324
8.059 ± 0.548
6.414 ± 0.442
6.414 ± 1.629
6.743 ± 0.517
2.303 ± 0.53
5.263 ± 0.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here