Arthrobacter phage Rings
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U4KQV6|A0A0U4KQV6_9CAUD RecA-like DNA recombinase OS=Arthrobacter phage Rings OX=1772313 GN=61 PE=4 SV=1
MM1 pKa = 7.65 TDD3 pKa = 3.07 ASNEE7 pKa = 3.89 AVEE10 pKa = 5.0 AEE12 pKa = 4.29 VAEE15 pKa = 4.42 APEE18 pKa = 4.36 EE19 pKa = 4.1 EE20 pKa = 4.76 VALQTVDD27 pKa = 4.7 NIVSPLRR34 pKa = 11.84 YY35 pKa = 8.08 TPDD38 pKa = 3.61 PCSTCHH44 pKa = 6.55
Molecular weight: 4.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.839
IPC2_protein 3.859
IPC_protein 3.643
Toseland 3.503
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.554
Rodwell 3.503
Grimsley 3.439
Solomon 3.567
Lehninger 3.528
Nozaki 3.783
DTASelect 3.834
Thurlkill 3.579
EMBOSS 3.567
Sillero 3.77
Patrickios 0.477
IPC_peptide 3.579
IPC2_peptide 3.745
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|A0A0U4KQS5|A0A0U4KQS5_9CAUD Uncharacterized protein OS=Arthrobacter phage Rings OX=1772313 GN=27 PE=4 SV=1
MM1 pKa = 7.08 TAITSTTMTTYY12 pKa = 7.51 TTTKK16 pKa = 10.42 KK17 pKa = 9.53 STTAAAGAAPVTARR31 pKa = 11.84 KK32 pKa = 9.92 RR33 pKa = 11.84 GTIVSLTKK41 pKa = 10.12 AQRR44 pKa = 11.84 RR45 pKa = 11.84 KK46 pKa = 9.91 LAGAHH51 pKa = 5.89 RR52 pKa = 11.84 ALYY55 pKa = 10.5 LDD57 pKa = 4.73 VVNQWVCKK65 pKa = 8.79 EE66 pKa = 4.61 DD67 pKa = 3.41 RR68 pKa = 11.84 TLWPCKK74 pKa = 9.11 TALHH78 pKa = 5.63 LHH80 pKa = 6.55 EE81 pKa = 5.36 LQQADD86 pKa = 3.89 RR87 pKa = 11.84 LVNNAKK93 pKa = 10.21 GKK95 pKa = 10.17 RR96 pKa = 11.84 KK97 pKa = 10.26 SPP99 pKa = 3.51
Molecular weight: 10.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.794
IPC_protein 10.277
Toseland 10.921
ProMoST 10.496
Dawson 10.979
Bjellqvist 10.628
Wikipedia 11.14
Rodwell 11.418
Grimsley 11.008
Solomon 11.082
Lehninger 11.067
Nozaki 10.891
DTASelect 10.628
Thurlkill 10.891
EMBOSS 11.316
Sillero 10.906
Patrickios 11.155
IPC_peptide 11.096
IPC2_peptide 9.487
IPC2.peptide.svr19 8.61
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
83
0
83
17263
33
1569
208.0
22.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.946 ± 0.641
0.713 ± 0.099
6.192 ± 0.206
7.056 ± 0.41
3.435 ± 0.121
7.606 ± 0.333
1.888 ± 0.179
5.121 ± 0.186
5.41 ± 0.246
7.791 ± 0.202
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.346 ± 0.114
4.356 ± 0.227
4.124 ± 0.207
3.731 ± 0.173
5.219 ± 0.207
6.065 ± 0.268
6.702 ± 0.303
7.247 ± 0.199
1.709 ± 0.177
3.342 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here