Arthrobacter phage Rings

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Amigovirus; unclassified Amigovirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0U4KQV6|A0A0U4KQV6_9CAUD RecA-like DNA recombinase OS=Arthrobacter phage Rings OX=1772313 GN=61 PE=4 SV=1
MM1 pKa = 7.65TDD3 pKa = 3.07ASNEE7 pKa = 3.89AVEE10 pKa = 5.0AEE12 pKa = 4.29VAEE15 pKa = 4.42APEE18 pKa = 4.36EE19 pKa = 4.1EE20 pKa = 4.76VALQTVDD27 pKa = 4.7NIVSPLRR34 pKa = 11.84YY35 pKa = 8.08TPDD38 pKa = 3.61PCSTCHH44 pKa = 6.55

Molecular weight:
4.73 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0U4KQS5|A0A0U4KQS5_9CAUD Uncharacterized protein OS=Arthrobacter phage Rings OX=1772313 GN=27 PE=4 SV=1
MM1 pKa = 7.08TAITSTTMTTYY12 pKa = 7.51TTTKK16 pKa = 10.42KK17 pKa = 9.53STTAAAGAAPVTARR31 pKa = 11.84KK32 pKa = 9.92RR33 pKa = 11.84GTIVSLTKK41 pKa = 10.12AQRR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.91LAGAHH51 pKa = 5.89RR52 pKa = 11.84ALYY55 pKa = 10.5LDD57 pKa = 4.73VVNQWVCKK65 pKa = 8.79EE66 pKa = 4.61DD67 pKa = 3.41RR68 pKa = 11.84TLWPCKK74 pKa = 9.11TALHH78 pKa = 5.63LHH80 pKa = 6.55EE81 pKa = 5.36LQQADD86 pKa = 3.89RR87 pKa = 11.84LVNNAKK93 pKa = 10.21GKK95 pKa = 10.17RR96 pKa = 11.84KK97 pKa = 10.26SPP99 pKa = 3.51

Molecular weight:
10.91 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

17263

33

1569

208.0

22.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.946 ± 0.641

0.713 ± 0.099

6.192 ± 0.206

7.056 ± 0.41

3.435 ± 0.121

7.606 ± 0.333

1.888 ± 0.179

5.121 ± 0.186

5.41 ± 0.246

7.791 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.114

4.356 ± 0.227

4.124 ± 0.207

3.731 ± 0.173

5.219 ± 0.207

6.065 ± 0.268

6.702 ± 0.303

7.247 ± 0.199

1.709 ± 0.177

3.342 ± 0.18

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski