Proteus phage PM 116

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Acadevirus; Proteus virus PM116

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0VKP9|A0A2D0VKP9_9CAUD DNA-directed DNA polymerase OS=Proteus phage PM 116 OX=1837877 PE=3 SV=1
MM1 pKa = 6.19TTYY4 pKa = 11.26NEE6 pKa = 4.23VLDD9 pKa = 4.21SAIEE13 pKa = 4.0SLKK16 pKa = 10.79EE17 pKa = 3.73YY18 pKa = 10.39IEE20 pKa = 4.86CNDD23 pKa = 3.41IQEE26 pKa = 4.33EE27 pKa = 4.84TYY29 pKa = 10.24EE30 pKa = 4.04IQDD33 pKa = 4.62AISEE37 pKa = 4.31IADD40 pKa = 3.51NAIPIYY46 pKa = 10.59NCDD49 pKa = 3.15IFSVFAEE56 pKa = 4.62GGISYY61 pKa = 10.84QMDD64 pKa = 3.54DD65 pKa = 2.94TGLIEE70 pKa = 4.74GCDD73 pKa = 3.63DD74 pKa = 4.35VIQILQMRR82 pKa = 11.84IYY84 pKa = 10.67EE85 pKa = 4.17EE86 pKa = 4.31LSNDD90 pKa = 3.65LYY92 pKa = 11.67HH93 pKa = 6.39MVRR96 pKa = 11.84GLINEE101 pKa = 4.11YY102 pKa = 10.44VYY104 pKa = 11.2SLEE107 pKa = 4.22EE108 pKa = 3.8EE109 pKa = 4.28

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0VJY5|A0A2D0VJY5_9CAUD Uncharacterized protein OS=Proteus phage PM 116 OX=1837877 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84YY3 pKa = 9.95NMKK6 pKa = 10.04LNKK9 pKa = 8.7MRR11 pKa = 11.84KK12 pKa = 4.74TAKK15 pKa = 10.24YY16 pKa = 9.39AMCYY20 pKa = 7.71GARR23 pKa = 11.84YY24 pKa = 6.8EE25 pKa = 4.15TFEE28 pKa = 6.64AIMLAKK34 pKa = 9.87KK35 pKa = 10.11AKK37 pKa = 9.5KK38 pKa = 10.05RR39 pKa = 11.84YY40 pKa = 8.83IKK42 pKa = 10.36KK43 pKa = 9.32AWKK46 pKa = 8.2QAKK49 pKa = 9.57VDD51 pKa = 4.09LANMGSYY58 pKa = 7.65GTPKK62 pKa = 10.6AILRR66 pKa = 11.84GADD69 pKa = 3.04RR70 pKa = 11.84IMSYY74 pKa = 10.53RR75 pKa = 11.84KK76 pKa = 9.57AYY78 pKa = 10.5SGDD81 pKa = 3.48VVDD84 pKa = 7.27FIIFDD89 pKa = 3.83EE90 pKa = 4.59VV91 pKa = 2.99

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

13646

50

1273

272.9

30.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.548 ± 0.428

1.129 ± 0.174

6.339 ± 0.243

7.16 ± 0.373

3.833 ± 0.134

7.526 ± 0.327

2.03 ± 0.2

6.126 ± 0.311

6.932 ± 0.309

8.112 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.202 ± 0.18

4.683 ± 0.239

3.166 ± 0.145

4.258 ± 0.249

5.159 ± 0.284

5.892 ± 0.284

5.467 ± 0.337

6.639 ± 0.298

1.334 ± 0.129

3.466 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski