Salinibacter phage M8CR30-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Holosalinivirus; Salinibacter virus M8CR30-2

Average proteome isoelectric point is 5.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6UGJ3|A0A2I6UGJ3_9CAUD DNA polymerase III beta subunit OS=Salinibacter phage M8CR30-2 OX=2681615 PE=3 SV=1
MM1 pKa = 7.62TSTTYY6 pKa = 11.16AVLSTTTQEE15 pKa = 4.27LEE17 pKa = 4.29TIEE20 pKa = 4.16AQSWGDD26 pKa = 3.17ALARR30 pKa = 11.84YY31 pKa = 9.63LEE33 pKa = 4.29EE34 pKa = 5.38EE35 pKa = 4.27GVEE38 pKa = 4.09WEE40 pKa = 4.38SLLGCVDD47 pKa = 4.01TTPSPHH53 pKa = 5.59YY54 pKa = 9.74TVDD57 pKa = 3.6LYY59 pKa = 10.76STSGEE64 pKa = 4.22EE65 pKa = 3.85EE66 pKa = 4.14TVLVMEE72 pKa = 5.55LDD74 pKa = 4.01DD75 pKa = 4.84LQVEE79 pKa = 4.5RR80 pKa = 11.84PKK82 pKa = 11.03YY83 pKa = 10.1FPNEE87 pKa = 3.63

Molecular weight: 9.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6UGM4|A0A2I6UGM4_9CAUD Uncharacterized protein OS=Salinibacter phage M8CR30-2 OX=2681615 PE=4 SV=1
MM1 pKa = 6.98NRR3 pKa = 11.84SMFCEE8 pKa = 3.85LRR10 pKa = 11.84RR11 pKa = 11.84CGKK14 pKa = 9.98HH15 pKa = 5.13KK16 pKa = 10.45GVWRR20 pKa = 11.84IYY22 pKa = 10.06DD23 pKa = 3.56RR24 pKa = 11.84EE25 pKa = 4.05RR26 pKa = 11.84EE27 pKa = 4.18ALSLRR32 pKa = 11.84LWFDD36 pKa = 3.31RR37 pKa = 11.84DD38 pKa = 3.17VAARR42 pKa = 11.84ALHH45 pKa = 5.99KK46 pKa = 10.59RR47 pKa = 3.6

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

9975

40

1954

243.3

26.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.366 ± 0.67

0.501 ± 0.132

7.82 ± 0.435

8.712 ± 0.501

2.637 ± 0.196

8.491 ± 0.659

1.574 ± 0.248

3.409 ± 0.226

2.657 ± 0.322

8.652 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.464 ± 0.238

2.647 ± 0.357

4.391 ± 0.49

4.401 ± 0.167

6.857 ± 0.578

7.599 ± 0.412

6.867 ± 0.758

7.118 ± 0.355

1.343 ± 0.148

2.496 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski