Sinomicrobium sp. N-1-3-6

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Sinomicrobium; unclassified Sinomicrobium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3695 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A329MWY0|A0A329MWY0_9FLAO DctM domain-containing protein OS=Sinomicrobium sp. N-1-3-6 OX=2219864 GN=DN748_10650 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.4NIYY5 pKa = 9.88KK6 pKa = 10.08YY7 pKa = 10.28IYY9 pKa = 9.32IIASIPLLLLGSCDD23 pKa = 4.44ALLDD27 pKa = 3.74QDD29 pKa = 3.94KK30 pKa = 10.69TDD32 pKa = 4.04FGKK35 pKa = 11.09GPNFVGFQNTGSSVSILVTGAEE57 pKa = 4.19VNIAVPVDD65 pKa = 3.6VVGPSVSKK73 pKa = 10.61LSQNIEE79 pKa = 3.87VTFEE83 pKa = 4.3VIDD86 pKa = 3.83SLTTAVEE93 pKa = 4.3GVNYY97 pKa = 10.15RR98 pKa = 11.84LEE100 pKa = 4.49SNAITLTPNDD110 pKa = 3.59WGDD113 pKa = 3.53LYY115 pKa = 10.82TAEE118 pKa = 4.7LPITIITEE126 pKa = 4.58GIEE129 pKa = 4.1VPLEE133 pKa = 3.75GDD135 pKa = 3.46APVIGLSVTGISSEE149 pKa = 4.2DD150 pKa = 3.02GVMINQKK157 pKa = 10.39SVVTEE162 pKa = 3.49VSLAYY167 pKa = 10.28SCPFDD172 pKa = 3.9INDD175 pKa = 3.42YY176 pKa = 10.91AGTYY180 pKa = 9.93LATTDD185 pKa = 3.47EE186 pKa = 4.38FGIYY190 pKa = 9.92ISDD193 pKa = 3.62PVPFEE198 pKa = 4.42VEE200 pKa = 4.03VGPGDD205 pKa = 3.75NQITLVNVAAHH216 pKa = 6.5PEE218 pKa = 4.17AYY220 pKa = 10.01DD221 pKa = 3.5VVVDD225 pKa = 4.37IDD227 pKa = 4.13PEE229 pKa = 4.25NGNLSIEE236 pKa = 4.17RR237 pKa = 11.84QPVLNSNNIGYY248 pKa = 8.26TYY250 pKa = 11.35GEE252 pKa = 4.45LRR254 pKa = 11.84WDD256 pKa = 3.67GTGTSIPSPGHH267 pKa = 5.39CVGVLDD273 pKa = 4.16IAPNFTVDD281 pKa = 3.63AGSFGPFRR289 pKa = 11.84IVFEE293 pKa = 4.33KK294 pKa = 10.69QQ295 pKa = 2.58

Molecular weight: 31.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A329N0K9|A0A329N0K9_9FLAO DoxX family protein OS=Sinomicrobium sp. N-1-3-6 OX=2219864 GN=DN748_09395 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.13RR10 pKa = 11.84KK11 pKa = 9.48RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.49HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.27RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 8.3KK41 pKa = 9.83LTVSSEE47 pKa = 3.9PRR49 pKa = 11.84HH50 pKa = 5.77KK51 pKa = 10.61KK52 pKa = 9.84

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3695

0

3695

1360127

25

3674

368.1

41.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.016 ± 0.038

0.717 ± 0.012

5.801 ± 0.028

6.889 ± 0.043

4.772 ± 0.027

7.536 ± 0.036

1.99 ± 0.02

6.636 ± 0.032

6.045 ± 0.041

9.118 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.018

4.961 ± 0.031

3.92 ± 0.022

3.209 ± 0.018

5.176 ± 0.03

6.128 ± 0.03

5.681 ± 0.029

6.581 ± 0.039

1.21 ± 0.017

4.32 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski