Citrus yellow vein-associated virus
Average proteome isoelectric point is 8.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4TYN1|S4TYN1_9VIRU RNA-dependent RNA polymerase OS=Citrus yellow vein-associated virus OX=1297894 GN=RdRp PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 5.76 PSSLPRR8 pKa = 11.84 ACWHH12 pKa = 6.7 HH13 pKa = 5.69 ARR15 pKa = 11.84 QVFRR19 pKa = 11.84 ASTSFSRR26 pKa = 11.84 EE27 pKa = 3.47 VVRR30 pKa = 11.84 AARR33 pKa = 11.84 RR34 pKa = 11.84 WVTMPTSRR42 pKa = 11.84 HH43 pKa = 5.9 AYY45 pKa = 10.06 DD46 pKa = 4.59 FSTSLGIVIAEE57 pKa = 3.76 PAARR61 pKa = 11.84 LRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 LPSVRR70 pKa = 11.84 KK71 pKa = 8.98 CAEE74 pKa = 3.73 KK75 pKa = 10.7 LVVHH79 pKa = 6.16 KK80 pKa = 10.82 QVDD83 pKa = 3.81 TLVDD87 pKa = 3.92 EE88 pKa = 4.65 WCSGIPNPDD97 pKa = 2.89 IVEE100 pKa = 4.32 VGWALRR106 pKa = 11.84 LRR108 pKa = 11.84 DD109 pKa = 3.99 RR110 pKa = 11.84 FGLPPASEE118 pKa = 4.22 PTRR121 pKa = 11.84 LSGEE125 pKa = 3.92 RR126 pKa = 11.84 WVLKK130 pKa = 9.92 QLNGVDD136 pKa = 3.9 PEE138 pKa = 4.35 SWNADD143 pKa = 3.26 LGRR146 pKa = 11.84 SVHH149 pKa = 5.76 IQGDD153 pKa = 3.93 YY154 pKa = 10.82 APGRR158 pKa = 11.84 NAHH161 pKa = 6.39 IAQVAATLWLTRR173 pKa = 11.84 TLHH176 pKa = 6.64 DD177 pKa = 3.77 KK178 pKa = 10.98 ALARR182 pKa = 11.84 HH183 pKa = 5.48 QGFSRR188 pKa = 11.84 FAVIGVDD195 pKa = 3.42 GLEE198 pKa = 4.24 AKK200 pKa = 10.01 AVPLASGLRR209 pKa = 11.84 LLPVPRR215 pKa = 11.84 PGQSRR220 pKa = 11.84 RR221 pKa = 11.84 LSQTLLLPTPRR232 pKa = 11.84 AQFVNHH238 pKa = 6.64 ACSANNLGRR247 pKa = 11.84 VMTTRR252 pKa = 11.84 VLQYY256 pKa = 10.5 KK257 pKa = 10.18 GRR259 pKa = 11.84 DD260 pKa = 3.56 PILPSSEE267 pKa = 3.61 ALHH270 pKa = 6.2 RR271 pKa = 11.84 LNLRR275 pKa = 11.84 IAEE278 pKa = 4.81 LYY280 pKa = 9.94 RR281 pKa = 11.84 SRR283 pKa = 11.84 PSTVYY288 pKa = 9.2 PLSYY292 pKa = 10.7 EE293 pKa = 4.16 GFLNCYY299 pKa = 8.89 EE300 pKa = 3.94 GRR302 pKa = 11.84 QRR304 pKa = 11.84 TRR306 pKa = 11.84 YY307 pKa = 8.48 AQAVEE312 pKa = 3.68 QLMRR316 pKa = 11.84 STLEE320 pKa = 4.01 PKK322 pKa = 10.12 DD323 pKa = 3.55 ARR325 pKa = 11.84 VEE327 pKa = 4.07 TFIKK331 pKa = 10.67 NEE333 pKa = 3.87 KK334 pKa = 9.72 FDD336 pKa = 3.53 WALKK340 pKa = 10.68 GEE342 pKa = 4.35 EE343 pKa = 4.24 ADD345 pKa = 3.84 PRR347 pKa = 11.84 AIQPRR352 pKa = 11.84 KK353 pKa = 8.79 PKK355 pKa = 10.37 YY356 pKa = 8.89 LAEE359 pKa = 4.02 VGRR362 pKa = 11.84 WFKK365 pKa = 10.32 PLEE368 pKa = 4.24 RR369 pKa = 11.84 IIYY372 pKa = 9.51 KK373 pKa = 10.36 DD374 pKa = 3.27 LSKK377 pKa = 10.93 RR378 pKa = 11.84 LYY380 pKa = 10.97 GEE382 pKa = 4.06 GAEE385 pKa = 4.01 PCIAKK390 pKa = 10.12 GLNALEE396 pKa = 4.46 SGATLRR402 pKa = 11.84 RR403 pKa = 11.84 KK404 pKa = 8.47 WEE406 pKa = 4.06 KK407 pKa = 10.23 FSSPVCVSLDD417 pKa = 3.2 ASRR420 pKa = 11.84 FDD422 pKa = 3.5 LHH424 pKa = 8.4 VSVGMLKK431 pKa = 9.18 FTHH434 pKa = 6.73 KK435 pKa = 10.79 LYY437 pKa = 10.56 DD438 pKa = 4.02 YY439 pKa = 9.48 YY440 pKa = 11.19 CKK442 pKa = 10.69 SPTLQRR448 pKa = 11.84 YY449 pKa = 7.53 LKK451 pKa = 8.41 WTLRR455 pKa = 11.84 NHH457 pKa = 6.55 GVASCKK463 pKa = 9.82 EE464 pKa = 3.64 LSYY467 pKa = 10.5 EE468 pKa = 4.07 YY469 pKa = 10.27 EE470 pKa = 4.18 VVGRR474 pKa = 11.84 RR475 pKa = 11.84 MSGDD479 pKa = 3.17 MDD481 pKa = 3.91 TALGNCVIMSILTWFMLSEE500 pKa = 4.28 LGIKK504 pKa = 10.13 HH505 pKa = 6.2 EE506 pKa = 4.74 LFDD509 pKa = 5.42 NGDD512 pKa = 3.56 DD513 pKa = 4.05 CLFICEE519 pKa = 4.39 SHH521 pKa = 7.08 DD522 pKa = 3.65 VPSPEE527 pKa = 5.54 VITNWFSDD535 pKa = 3.25 FGFVVRR541 pKa = 11.84 LEE543 pKa = 4.33 GVTSVFEE550 pKa = 4.61 RR551 pKa = 11.84 IEE553 pKa = 4.15 FCQTSPVWTEE563 pKa = 4.19 RR564 pKa = 11.84 GWLMCRR570 pKa = 11.84 NIKK573 pKa = 10.27 SLSKK577 pKa = 10.89 DD578 pKa = 3.19 LTNVNSCTGSTIEE591 pKa = 3.89 YY592 pKa = 6.55 THH594 pKa = 6.68 WLKK597 pKa = 11.0 AVGKK601 pKa = 9.92 CGSILNAGVPIFQSFHH617 pKa = 6.42 NMLEE621 pKa = 4.05 RR622 pKa = 11.84 LGTNSRR628 pKa = 11.84 IDD630 pKa = 2.92 RR631 pKa = 11.84 GVFFKK636 pKa = 11.0 SGLVNLIRR644 pKa = 11.84 GMDD647 pKa = 3.63 RR648 pKa = 11.84 QPDD651 pKa = 3.44 VDD653 pKa = 3.35 ITTSARR659 pKa = 11.84 LSFEE663 pKa = 4.05 VAFGITPGMQLAIEE677 pKa = 4.82 RR678 pKa = 11.84 YY679 pKa = 8.31 YY680 pKa = 11.29 DD681 pKa = 3.5 SVMGSLSKK689 pKa = 10.56 IEE691 pKa = 4.06 TTKK694 pKa = 10.43 WPIEE698 pKa = 3.71 LRR700 pKa = 11.84 KK701 pKa = 9.8 EE702 pKa = 4.1 YY703 pKa = 10.54 EE704 pKa = 4.77 HH705 pKa = 7.63 GSEE708 pKa = 3.94 WYY710 pKa = 9.73 EE711 pKa = 3.81 DD712 pKa = 3.71 LGVLGG717 pKa = 4.78
Molecular weight: 81.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.485
IPC2_protein 8.39
IPC_protein 8.258
Toseland 8.521
ProMoST 8.799
Dawson 9.092
Bjellqvist 9.18
Wikipedia 9.18
Rodwell 9.194
Grimsley 8.799
Solomon 9.151
Lehninger 9.136
Nozaki 9.341
DTASelect 8.99
Thurlkill 9.077
EMBOSS 9.253
Sillero 9.311
Patrickios 4.673
IPC_peptide 9.151
IPC2_peptide 8.097
IPC2.peptide.svr19 7.829
Protein with the highest isoelectric point:
>tr|S4TYN1|S4TYN1_9VIRU RNA-dependent RNA polymerase OS=Citrus yellow vein-associated virus OX=1297894 GN=RdRp PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 5.76 PSSLPRR8 pKa = 11.84 ACWHH12 pKa = 6.7 HH13 pKa = 5.69 ARR15 pKa = 11.84 QVFRR19 pKa = 11.84 ASTSFSRR26 pKa = 11.84 EE27 pKa = 3.47 VVRR30 pKa = 11.84 AARR33 pKa = 11.84 RR34 pKa = 11.84 WVTMPTSRR42 pKa = 11.84 HH43 pKa = 5.9 AYY45 pKa = 10.06 DD46 pKa = 4.59 FSTSLGIVIAEE57 pKa = 3.76 PAARR61 pKa = 11.84 LRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 LPSVRR70 pKa = 11.84 KK71 pKa = 8.98 CAEE74 pKa = 3.73 KK75 pKa = 10.7 LVVHH79 pKa = 6.16 KK80 pKa = 10.82 QVDD83 pKa = 3.81 TLVDD87 pKa = 3.92 EE88 pKa = 4.65 WCSGIPNPDD97 pKa = 2.89 IVEE100 pKa = 4.32 VGWALRR106 pKa = 11.84 LRR108 pKa = 11.84 DD109 pKa = 3.99 RR110 pKa = 11.84 FGLPPASEE118 pKa = 4.22 PTRR121 pKa = 11.84 LSGEE125 pKa = 3.92 RR126 pKa = 11.84 WVLKK130 pKa = 9.92 QLNGVDD136 pKa = 3.9 PEE138 pKa = 4.35 SWNADD143 pKa = 3.26 LGRR146 pKa = 11.84 SVHH149 pKa = 5.76 IQGDD153 pKa = 3.93 YY154 pKa = 10.82 APGRR158 pKa = 11.84 NAHH161 pKa = 6.39 IAQVAATLWLTRR173 pKa = 11.84 TLHH176 pKa = 6.64 DD177 pKa = 3.77 KK178 pKa = 10.98 ALARR182 pKa = 11.84 HH183 pKa = 5.38 QGFRR187 pKa = 11.84 DD188 pKa = 3.62 LQQ190 pKa = 3.74
Molecular weight: 21.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.297
IPC_protein 10.014
Toseland 10.482
ProMoST 10.189
Dawson 10.57
Bjellqvist 10.306
Wikipedia 10.789
Rodwell 10.643
Grimsley 10.613
Solomon 10.716
Lehninger 10.687
Nozaki 10.496
DTASelect 10.292
Thurlkill 10.482
EMBOSS 10.891
Sillero 10.511
Patrickios 10.409
IPC_peptide 10.716
IPC2_peptide 9.516
IPC2.peptide.svr19 8.523
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
907
190
717
453.5
51.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.497 ± 1.509
2.095 ± 0.257
4.851 ± 0.467
5.954 ± 0.868
3.638 ± 0.502
6.395 ± 0.564
2.867 ± 0.669
4.079 ± 0.459
4.19 ± 0.776
10.254 ± 0.389
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.764 ± 0.354
2.867 ± 0.379
5.402 ± 0.455
2.867 ± 0.407
9.592 ± 1.252
7.828 ± 0.229
5.072 ± 0.167
7.387 ± 0.515
2.756 ± 0.462
2.646 ± 0.794
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here