Apis mellifera associated microvirus 45

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5U1|A0A3Q8U5U1_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 45 OX=2494775 PE=4 SV=1
MM1 pKa = 7.97AKK3 pKa = 10.14RR4 pKa = 11.84KK5 pKa = 7.46QTVAEE10 pKa = 3.9WLGLIRR16 pKa = 11.84KK17 pKa = 9.09QDD19 pKa = 3.47DD20 pKa = 3.46EE21 pKa = 4.3GRR23 pKa = 11.84EE24 pKa = 4.24IVDD27 pKa = 3.86PTPMGLPAGFKK38 pKa = 10.41RR39 pKa = 11.84PEE41 pKa = 3.8TLAEE45 pKa = 3.78QVRR48 pKa = 11.84RR49 pKa = 11.84LVRR52 pKa = 11.84TEE54 pKa = 4.18KK55 pKa = 10.91LVDD58 pKa = 4.34DD59 pKa = 5.49DD60 pKa = 6.27DD61 pKa = 4.72VEE63 pKa = 4.5SWEE66 pKa = 4.14EE67 pKa = 3.87ANDD70 pKa = 3.64FDD72 pKa = 5.86VDD74 pKa = 4.76DD75 pKa = 5.79DD76 pKa = 4.93FDD78 pKa = 4.28PASPFEE84 pKa = 4.33TVFDD88 pKa = 4.37PEE90 pKa = 4.68LGRR93 pKa = 11.84EE94 pKa = 4.17LTPNDD99 pKa = 4.33YY100 pKa = 10.6KK101 pKa = 10.72EE102 pKa = 3.76HH103 pKa = 6.03WPNIKK108 pKa = 10.53EE109 pKa = 4.03EE110 pKa = 4.23MQKK113 pKa = 10.3HH114 pKa = 5.46LRR116 pKa = 11.84NKK118 pKa = 9.05YY119 pKa = 8.21RR120 pKa = 11.84QEE122 pKa = 4.04EE123 pKa = 4.48LEE125 pKa = 4.16HH126 pKa = 7.75AEE128 pKa = 4.13QQLLEE133 pKa = 4.06NAYY136 pKa = 9.78KK137 pKa = 10.51RR138 pKa = 11.84GAGVSPAPSKK148 pKa = 10.13PAEE151 pKa = 4.22PQAPPEE157 pKa = 4.21KK158 pKa = 10.13STT160 pKa = 3.79

Molecular weight: 18.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UU70|A0A3S8UU70_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 45 OX=2494775 PE=4 SV=1
MM1 pKa = 7.48GPFFRR6 pKa = 11.84TRR8 pKa = 11.84CRR10 pKa = 11.84PSRR13 pKa = 11.84WSTVTTDD20 pKa = 2.89TGGFMARR27 pKa = 11.84KK28 pKa = 8.52HH29 pKa = 5.18RR30 pKa = 11.84HH31 pKa = 4.27IKK33 pKa = 7.7TRR35 pKa = 11.84RR36 pKa = 11.84DD37 pKa = 3.43VPQSSLTPSALPEE50 pKa = 4.08NFTAIRR56 pKa = 11.84SNFLFEE62 pKa = 4.98DD63 pKa = 3.27RR64 pKa = 11.84RR65 pKa = 11.84IFNPEE70 pKa = 3.38PTVARR75 pKa = 11.84TPRR78 pKa = 11.84RR79 pKa = 11.84VVRR82 pKa = 11.84LQLPKK87 pKa = 10.4VPRR90 pKa = 11.84LVARR94 pKa = 11.84QATRR98 pKa = 11.84SPIRR102 pKa = 11.84RR103 pKa = 11.84SRR105 pKa = 11.84NGYY108 pKa = 8.51VRR110 pKa = 11.84SAPSPHH116 pKa = 6.8GYY118 pKa = 10.95ALLKK122 pKa = 10.25SGRR125 pKa = 11.84HH126 pKa = 5.27RR127 pKa = 11.84GLVFSVPEE135 pKa = 3.82AVPICVRR142 pKa = 11.84RR143 pKa = 11.84KK144 pKa = 9.2RR145 pKa = 11.84RR146 pKa = 11.84RR147 pKa = 11.84EE148 pKa = 3.6VLLALGKK155 pKa = 10.81GGGGKK160 pKa = 9.52RR161 pKa = 11.84RR162 pKa = 11.84PRR164 pKa = 11.84YY165 pKa = 9.94NKK167 pKa = 9.79FSNVRR172 pKa = 11.84CC173 pKa = 3.82

Molecular weight: 19.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1462

160

509

292.4

32.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.866 ± 0.667

0.821 ± 0.502

4.72 ± 0.872

6.019 ± 0.933

3.967 ± 0.338

7.524 ± 0.512

2.462 ± 0.121

3.42 ± 0.311

5.062 ± 0.652

7.729 ± 0.735

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.257 ± 0.301

3.557 ± 0.481

7.182 ± 0.763

3.899 ± 0.423

8.276 ± 1.569

7.866 ± 0.697

5.677 ± 0.379

6.293 ± 0.801

2.052 ± 0.214

3.352 ± 0.547
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski