Staphylococcus phage phiPVL-CN125

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Peeveelvirus; Staphylococcus virus CN125

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5I631|C5I631_9CAUD Anti-repressor OS=Staphylococcus phage phiPVL-CN125 OX=648017 GN=ant PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.25NYY4 pKa = 10.4YY5 pKa = 9.86HH6 pKa = 7.24LLSFDD11 pKa = 5.04DD12 pKa = 5.61DD13 pKa = 4.5LANDD17 pKa = 4.17AANDD21 pKa = 4.01LLKK24 pKa = 10.8EE25 pKa = 4.08GWDD28 pKa = 3.34IVHH31 pKa = 6.66VGTKK35 pKa = 9.51LVKK38 pKa = 10.36ILDD41 pKa = 3.92NGQAYY46 pKa = 10.82YY47 pKa = 8.91NTEE50 pKa = 3.97YY51 pKa = 11.47VLGGTKK57 pKa = 9.45NQYY60 pKa = 9.36EE61 pKa = 4.83KK62 pKa = 11.2YY63 pKa = 10.75LEE65 pKa = 4.8DD66 pKa = 4.04CQQSEE71 pKa = 4.31LDD73 pKa = 3.76YY74 pKa = 11.3FF75 pKa = 4.23

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5I625|C5I625_9CAUD Transcription regulator OS=Staphylococcus phage phiPVL-CN125 OX=648017 GN=CUR003 PE=4 SV=1
MM1 pKa = 7.81LSPFIFSAIHH11 pKa = 5.75SLGLRR16 pKa = 11.84PLKK19 pKa = 10.83ASGGTFNFSLSIATSSKK36 pKa = 10.19FPAEE40 pKa = 4.04TLPKK44 pKa = 9.54YY45 pKa = 8.26GTSISLSNSGKK56 pKa = 8.43TISSISPNRR65 pKa = 3.46

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12049

35

1440

185.4

21.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.104 ± 0.518

0.622 ± 0.124

5.967 ± 0.318

7.204 ± 0.479

3.975 ± 0.216

5.934 ± 0.601

1.834 ± 0.14

7.909 ± 0.27

9.503 ± 0.279

7.735 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.155

6.839 ± 0.294

2.598 ± 0.173

4.025 ± 0.231

4.216 ± 0.218

6.274 ± 0.276

6.075 ± 0.282

5.81 ± 0.216

1.154 ± 0.15

4.523 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski