Opitutaceae bacterium TSB47

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; unclassified Opitutaceae

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4993 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A178IN72|A0A178IN72_9BACT Beta-N-acetylhexosaminidase OS=Opitutaceae bacterium TSB47 OX=1184151 GN=AW736_07695 PE=3 SV=1
MM1 pKa = 7.42IKK3 pKa = 10.4NVFKK7 pKa = 11.09LGGVAISALLLSAPAFADD25 pKa = 3.36IPLHH29 pKa = 6.18QNFSANGYY37 pKa = 8.7VAGSYY42 pKa = 7.79MWTDD46 pKa = 3.51KK47 pKa = 11.24GDD49 pKa = 3.62SDD51 pKa = 5.83HH52 pKa = 7.28YY53 pKa = 11.52DD54 pKa = 3.43LDD56 pKa = 3.89VAKK59 pKa = 10.7LGFSINYY66 pKa = 9.21APTKK70 pKa = 10.47AYY72 pKa = 9.73ISLFYY77 pKa = 11.02DD78 pKa = 3.47GDD80 pKa = 4.1EE81 pKa = 4.32INVLDD86 pKa = 5.22AYY88 pKa = 11.18VDD90 pKa = 3.67YY91 pKa = 11.35DD92 pKa = 3.96FGNGAVVTGGRR103 pKa = 11.84FLSFLGFEE111 pKa = 4.78AFDD114 pKa = 4.24APNMYY119 pKa = 10.17QISYY123 pKa = 11.16ANGDD127 pKa = 3.89LFGGSALEE135 pKa = 3.98IAPIPAYY142 pKa = 10.17HH143 pKa = 6.45SGVKK147 pKa = 9.62VVYY150 pKa = 10.73SDD152 pKa = 4.48DD153 pKa = 3.24AWSAGVAFLDD163 pKa = 4.18SVYY166 pKa = 11.11GGLKK170 pKa = 10.35GDD172 pKa = 4.99GEE174 pKa = 4.54VEE176 pKa = 4.32DD177 pKa = 4.19NFGVEE182 pKa = 4.31AFFSFTGVEE191 pKa = 4.01KK192 pKa = 10.1LTLFGGIAFQSEE204 pKa = 4.3DD205 pKa = 4.2DD206 pKa = 4.91DD207 pKa = 4.17PTDD210 pKa = 4.35GYY212 pKa = 9.6TSPEE216 pKa = 3.28IMTFNFWASYY226 pKa = 9.65EE227 pKa = 4.29ISDD230 pKa = 3.35KK231 pKa = 10.81WLVAGEE237 pKa = 4.4FTFSDD242 pKa = 3.29QKK244 pKa = 11.74DD245 pKa = 3.8YY246 pKa = 11.14MDD248 pKa = 4.66GYY250 pKa = 11.4NWLALAKK257 pKa = 10.37YY258 pKa = 10.02AITPKK263 pKa = 9.6ISAVFRR269 pKa = 11.84VSGEE273 pKa = 3.96DD274 pKa = 3.12VDD276 pKa = 4.8YY277 pKa = 10.92EE278 pKa = 4.59DD279 pKa = 6.15PIEE282 pKa = 4.29TDD284 pKa = 3.14PSFLKK289 pKa = 9.88FTLAPAYY296 pKa = 8.68TITDD300 pKa = 3.79NLSVTAEE307 pKa = 3.84VSYY310 pKa = 11.43YY311 pKa = 10.54DD312 pKa = 3.59YY313 pKa = 11.33KK314 pKa = 11.48DD315 pKa = 3.69YY316 pKa = 11.31VVKK319 pKa = 10.76DD320 pKa = 3.69DD321 pKa = 4.05VFVGVQAIFKK331 pKa = 9.48FF332 pKa = 3.98

Molecular weight: 36.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A178IGB6|A0A178IGB6_9BACT Uncharacterized protein OS=Opitutaceae bacterium TSB47 OX=1184151 GN=AW736_18890 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 8.83PTFRR6 pKa = 11.84PHH8 pKa = 5.5RR9 pKa = 11.84KK10 pKa = 9.07KK11 pKa = 10.33RR12 pKa = 11.84VRR14 pKa = 11.84KK15 pKa = 8.27IGFRR19 pKa = 11.84ARR21 pKa = 11.84SATKK25 pKa = 10.17GGRR28 pKa = 11.84KK29 pKa = 9.08VLANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84VGRR39 pKa = 11.84KK40 pKa = 8.97RR41 pKa = 11.84LTVAA45 pKa = 4.08

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4993

0

4993

2146511

41

16945

429.9

46.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.216 ± 0.062

0.871 ± 0.015

5.384 ± 0.023

4.915 ± 0.054

3.917 ± 0.025

9.211 ± 0.117

2.105 ± 0.021

4.874 ± 0.026

3.437 ± 0.044

9.632 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.863 ± 0.021

3.537 ± 0.045

5.235 ± 0.064

2.922 ± 0.025

6.704 ± 0.065

5.878 ± 0.061

6.302 ± 0.083

6.686 ± 0.028

1.624 ± 0.017

2.689 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski