Camel associated porprismacovirus 4
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1EKX0|A0A0A1EKX0_9VIRU Putative replicase protein OS=Camel associated porprismacovirus 4 OX=2170108 GN=rep PE=4 SV=1
MM1 pKa = 7.31 ATQTVHH7 pKa = 5.84 ATYY10 pKa = 10.67 QEE12 pKa = 4.36 IYY14 pKa = 10.55 DD15 pKa = 4.48 LSTKK19 pKa = 10.35 PGTLSVIGVHH29 pKa = 5.71 TPTGRR34 pKa = 11.84 KK35 pKa = 8.95 PYY37 pKa = 11.35 DD38 pKa = 3.17 MLQGFFRR45 pKa = 11.84 QFKK48 pKa = 8.4 KK49 pKa = 10.75 YY50 pKa = 9.66 KK51 pKa = 9.06 YY52 pKa = 9.92 NGITRR57 pKa = 11.84 LVLQPAAQLPADD69 pKa = 4.21 PLQVSLEE76 pKa = 4.12 AGEE79 pKa = 4.62 NLDD82 pKa = 5.42 PRR84 pKa = 11.84 DD85 pKa = 3.89 LLNPIMFHH93 pKa = 6.31 GAHH96 pKa = 5.75 GTDD99 pKa = 3.17 INAALNVIYY108 pKa = 10.34 KK109 pKa = 10.05 KK110 pKa = 10.47 QGFNFQTNSTDD121 pKa = 3.78 TIDD124 pKa = 4.62 LSMFANVEE132 pKa = 4.19 GEE134 pKa = 4.29 VMDD137 pKa = 4.09 SVVVEE142 pKa = 3.86 NMYY145 pKa = 11.01 YY146 pKa = 10.37 SALSDD151 pKa = 3.48 PSFRR155 pKa = 11.84 KK156 pKa = 10.25 YY157 pKa = 10.5 GVQEE161 pKa = 4.27 VIDD164 pKa = 4.29 IGPLVPLVFRR174 pKa = 11.84 VNTNMYY180 pKa = 9.66 FGPQMSYY187 pKa = 10.75 PRR189 pKa = 11.84 DD190 pKa = 3.5 VFPNVGTSSPNGGFSVSDD208 pKa = 3.42 IPSEE212 pKa = 4.13 VWNGSSNVSAQTYY225 pKa = 7.22 GTDD228 pKa = 3.35 MTDD231 pKa = 3.03 MQIFTNGVTEE241 pKa = 4.41 LSWMPTRR248 pKa = 11.84 NAKK251 pKa = 9.75 PSSVGTDD258 pKa = 3.53 GSPTNTAFVTVPKK271 pKa = 10.12 LYY273 pKa = 9.47 MGAIIMPPAYY283 pKa = 9.73 NQRR286 pKa = 11.84 LFYY289 pKa = 10.6 RR290 pKa = 11.84 CVITHH295 pKa = 6.77 SFTFSDD301 pKa = 3.83 FGTYY305 pKa = 9.9 FDD307 pKa = 4.71 GGLNGGSEE315 pKa = 4.57 CYY317 pKa = 10.44 YY318 pKa = 11.43 NNLDD322 pKa = 3.69 YY323 pKa = 11.01 VAPATMSSGNLDD335 pKa = 3.75 SLNLTDD341 pKa = 4.97 VEE343 pKa = 4.59 GTMRR347 pKa = 11.84 TSGVMM352 pKa = 3.2
Molecular weight: 38.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.52
IPC2_protein 4.749
IPC_protein 4.698
Toseland 4.546
ProMoST 4.838
Dawson 4.685
Bjellqvist 4.825
Wikipedia 4.596
Rodwell 4.558
Grimsley 4.457
Solomon 4.685
Lehninger 4.635
Nozaki 4.8
DTASelect 5.029
Thurlkill 4.571
EMBOSS 4.609
Sillero 4.838
Patrickios 2.715
IPC_peptide 4.685
IPC2_peptide 4.825
IPC2.peptide.svr19 4.799
Protein with the highest isoelectric point:
>tr|A0A0A1EKX0|A0A0A1EKX0_9VIRU Putative replicase protein OS=Camel associated porprismacovirus 4 OX=2170108 GN=rep PE=4 SV=1
MM1 pKa = 7.1 LTIPAKK7 pKa = 10.35 RR8 pKa = 11.84 GEE10 pKa = 3.85 FDD12 pKa = 3.79 FYY14 pKa = 11.26 HH15 pKa = 6.21 WLKK18 pKa = 10.88 LFEE21 pKa = 3.97 VWNVKK26 pKa = 9.5 KK27 pKa = 10.12 HH28 pKa = 4.32 VVGLEE33 pKa = 3.85 EE34 pKa = 4.28 GKK36 pKa = 10.26 NGYY39 pKa = 8.28 KK40 pKa = 9.77 HH41 pKa = 4.48 WQARR45 pKa = 11.84 IQLSGEE51 pKa = 4.06 GKK53 pKa = 10.12 QIIGGSEE60 pKa = 3.41 ISFFQYY66 pKa = 10.06 MKK68 pKa = 10.0 IYY70 pKa = 9.46 YY71 pKa = 8.34 PKK73 pKa = 10.66 AHH75 pKa = 6.86 IEE77 pKa = 4.06 EE78 pKa = 5.02 ASNTWEE84 pKa = 4.12 YY85 pKa = 10.62 EE86 pKa = 3.99 RR87 pKa = 11.84 KK88 pKa = 9.62 EE89 pKa = 4.23 GKK91 pKa = 9.45 FWTSEE96 pKa = 3.78 DD97 pKa = 3.63 TASILAVRR105 pKa = 11.84 FGSLRR110 pKa = 11.84 PEE112 pKa = 3.91 QKK114 pKa = 10.41 KK115 pKa = 9.82 ILQILEE121 pKa = 4.21 SQGDD125 pKa = 3.72 RR126 pKa = 11.84 EE127 pKa = 4.01 IDD129 pKa = 3.26 VWLDD133 pKa = 2.99 PSGNHH138 pKa = 5.31 GKK140 pKa = 9.4 SWLTVHH146 pKa = 6.55 LWEE149 pKa = 4.48 TGRR152 pKa = 11.84 ALVVPRR158 pKa = 11.84 SSTTAEE164 pKa = 4.04 KK165 pKa = 10.66 LSAFICSSWKK175 pKa = 10.52 GEE177 pKa = 4.2 PIVIIDD183 pKa = 3.9 IPRR186 pKa = 11.84 STKK189 pKa = 10.16 VSGSLLEE196 pKa = 4.23 TMEE199 pKa = 4.12 EE200 pKa = 4.16 LKK202 pKa = 11.09 DD203 pKa = 3.59 GLVFDD208 pKa = 4.17 HH209 pKa = 7.47 RR210 pKa = 11.84 YY211 pKa = 7.63 TGRR214 pKa = 11.84 TRR216 pKa = 11.84 NVRR219 pKa = 11.84 GVKK222 pKa = 10.51 VMVFTNSEE230 pKa = 4.15 LPLKK234 pKa = 10.42 KK235 pKa = 10.14 LSKK238 pKa = 10.58 DD239 pKa = 2.57 RR240 pKa = 11.84 WRR242 pKa = 11.84 LHH244 pKa = 7.0 GIACDD249 pKa = 4.01 GSLTT253 pKa = 3.83
Molecular weight: 29.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.466
IPC2_protein 8.331
IPC_protein 8.287
Toseland 9.048
ProMoST 8.785
Dawson 9.297
Bjellqvist 9.048
Wikipedia 9.428
Rodwell 9.575
Grimsley 9.311
Solomon 9.399
Lehninger 9.37
Nozaki 9.209
DTASelect 8.99
Thurlkill 9.165
EMBOSS 9.472
Sillero 9.297
Patrickios 5.054
IPC_peptide 9.385
IPC2_peptide 7.629
IPC2.peptide.svr19 7.655
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
605
253
352
302.5
33.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.628 ± 0.414
0.661 ± 0.079
5.124 ± 0.717
5.455 ± 1.985
4.793 ± 0.515
8.43 ± 0.079
2.149 ± 0.62
5.124 ± 0.977
5.289 ± 2.086
7.769 ± 0.81
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.306 ± 1.056
4.959 ± 1.584
5.124 ± 1.201
3.471 ± 0.431
4.298 ± 0.999
8.264 ± 0.022
7.438 ± 1.166
7.438 ± 0.682
1.983 ± 1.206
4.298 ± 0.937
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here