Kumasi rhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ledantevirus; Kumasi ledantevirus

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C4MKA3|A0A0C4MKA3_9RHAB G OS=Kumasi rhabdovirus OX=1537975 PE=4 SV=1
MM1 pKa = 7.39SFSKK5 pKa = 10.85PEE7 pKa = 3.66AVYY10 pKa = 8.34RR11 pKa = 11.84TCNHH15 pKa = 5.95KK16 pKa = 9.63QVKK19 pKa = 9.01PVLPQEE25 pKa = 4.29TTPIQYY31 pKa = 10.67ASAWFRR37 pKa = 11.84ANRR40 pKa = 11.84ARR42 pKa = 11.84KK43 pKa = 6.4PTLVVGLKK51 pKa = 9.84NVPLVQTLQHH61 pKa = 6.73AAQAMIEE68 pKa = 4.01GRR70 pKa = 11.84LTGDD74 pKa = 3.0IAAATLYY81 pKa = 10.72KK82 pKa = 10.09IFKK85 pKa = 8.95SQKK88 pKa = 9.27QRR90 pKa = 11.84LSLDD94 pKa = 3.1WVSFGVVIGNADD106 pKa = 3.8TEE108 pKa = 4.47VCPWDD113 pKa = 3.9LVNVDD118 pKa = 4.24EE119 pKa = 6.92RR120 pKa = 11.84DD121 pKa = 3.53TQLPNEE127 pKa = 4.53QINDD131 pKa = 3.67QIRR134 pKa = 11.84EE135 pKa = 3.81EE136 pKa = 4.12DD137 pKa = 3.78APWIAFYY144 pKa = 10.61ICFLYY149 pKa = 10.25RR150 pKa = 11.84YY151 pKa = 9.46SKK153 pKa = 9.9ATHH156 pKa = 5.68TDD158 pKa = 3.36YY159 pKa = 11.7RR160 pKa = 11.84DD161 pKa = 3.41MLYY164 pKa = 8.8NTAKK168 pKa = 10.64DD169 pKa = 3.67HH170 pKa = 6.98AKK172 pKa = 10.27HH173 pKa = 5.83LSQRR177 pKa = 11.84AMDD180 pKa = 3.98IQPGSLAQMKK190 pKa = 10.25NVGLDD195 pKa = 3.24ANYY198 pKa = 10.41NCAIACIDD206 pKa = 3.67MFHH209 pKa = 7.04HH210 pKa = 6.74KK211 pKa = 10.15FKK213 pKa = 10.87NLPLSAVRR221 pKa = 11.84YY222 pKa = 6.23GTLPSRR228 pKa = 11.84FKK230 pKa = 10.97DD231 pKa = 3.78CSALTTLNHH240 pKa = 5.35ITKK243 pKa = 8.92LTGLAIEE250 pKa = 5.19QFMLWVFSSKK260 pKa = 10.02MADD263 pKa = 3.83EE264 pKa = 5.24LDD266 pKa = 3.82QMSKK270 pKa = 10.53PDD272 pKa = 3.65EE273 pKa = 4.39EE274 pKa = 5.59LDD276 pKa = 3.62QGDD279 pKa = 4.0SYY281 pKa = 10.48VAYY284 pKa = 9.39MRR286 pKa = 11.84EE287 pKa = 4.09LGVSDD292 pKa = 4.33RR293 pKa = 11.84SPYY296 pKa = 9.8SAQANPSFSLFCHH309 pKa = 5.41VVGALLGSKK318 pKa = 9.96RR319 pKa = 11.84SKK321 pKa = 8.86NARR324 pKa = 11.84MGAEE328 pKa = 3.66VDD330 pKa = 3.91TVNSVLNGKK339 pKa = 8.88IVAYY343 pKa = 10.49VLGTRR348 pKa = 11.84PSFTKK353 pKa = 10.24EE354 pKa = 3.49YY355 pKa = 9.71STDD358 pKa = 3.56KK359 pKa = 11.41EE360 pKa = 4.01EE361 pKa = 5.35DD362 pKa = 3.35PTGTAEE368 pKa = 4.1EE369 pKa = 4.74DD370 pKa = 3.66VTPGEE375 pKa = 4.61IPNGANPDD383 pKa = 2.87EE384 pKa = 4.27WFAYY388 pKa = 10.35LSIKK392 pKa = 10.61GFVLPDD398 pKa = 3.59EE399 pKa = 4.43VVHH402 pKa = 5.27WVKK405 pKa = 9.91TRR407 pKa = 11.84VQLLTDD413 pKa = 3.46VRR415 pKa = 11.84DD416 pKa = 3.81GTVGKK421 pKa = 9.62FLKK424 pKa = 9.27TQGG427 pKa = 3.4

Molecular weight: 47.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C4ME19|A0A0C4ME19_9RHAB GDP polyribonucleotidyltransferase OS=Kumasi rhabdovirus OX=1537975 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 4.39KK3 pKa = 10.35MKK5 pKa = 10.92SSLLAKK11 pKa = 9.54MMKK14 pKa = 10.53KK15 pKa = 9.73MMTEE19 pKa = 3.81EE20 pKa = 3.95VEE22 pKa = 3.97MRR24 pKa = 11.84ILGLIPYY31 pKa = 9.39LKK33 pKa = 9.72TPNSLEE39 pKa = 4.43GDD41 pKa = 3.27WKK43 pKa = 7.87MTRR46 pKa = 11.84PEE48 pKa = 3.86RR49 pKa = 11.84RR50 pKa = 11.84LGRR53 pKa = 11.84YY54 pKa = 7.67HH55 pKa = 5.42WRR57 pKa = 11.84KK58 pKa = 9.23RR59 pKa = 11.84VNQLRR64 pKa = 11.84YY65 pKa = 9.5RR66 pKa = 11.84VFLLRR71 pKa = 11.84LL72 pKa = 3.53

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3458

22

2122

494.0

56.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.685 ± 0.921

2.371 ± 0.219

5.234 ± 0.348

5.726 ± 0.173

4.078 ± 0.321

6.333 ± 0.515

3.036 ± 0.378

6.073 ± 0.653

6.333 ± 0.484

9.746 ± 1.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.33

4.627 ± 0.49

4.858 ± 0.477

3.152 ± 0.365

5.552 ± 0.642

7.577 ± 0.869

6.189 ± 0.43

6.391 ± 0.901

2.198 ± 0.286

3.412 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski