Oat golden stripe virus
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9IWA9|Q9IWA9_9VIRU Methyltransferase/RNA helicase OS=Oat golden stripe virus OX=45103 GN=ORF1 readthrough PE=4 SV=1
MM1 pKa = 7.74 PVTPGYY7 pKa = 8.96 TGYY10 pKa = 10.93 NKK12 pKa = 10.06 EE13 pKa = 4.36 LNVMANTHH21 pKa = 5.47 AFIRR25 pKa = 11.84 MSTLMNQIEE34 pKa = 4.87 GWQSTRR40 pKa = 11.84 ASVLSHH46 pKa = 6.57 LGIMLNAVSKK56 pKa = 10.73 LGEE59 pKa = 3.92 RR60 pKa = 11.84 NFFSRR65 pKa = 11.84 NKK67 pKa = 10.33 RR68 pKa = 11.84 FGTHH72 pKa = 5.06 TQDD75 pKa = 3.63 GDD77 pKa = 4.7 EE78 pKa = 4.48 IFCDD82 pKa = 3.64 LGGEE86 pKa = 4.24 AVGQVLGRR94 pKa = 11.84 LTVALQPAKK103 pKa = 11.03 GEE105 pKa = 4.22 GTQTRR110 pKa = 11.84 NSKK113 pKa = 10.51 RR114 pKa = 11.84 GVPPAAGQVEE124 pKa = 4.56 GEE126 pKa = 4.27 EE127 pKa = 4.43 QGQSDD132 pKa = 4.05 QTLQIANAVSDD143 pKa = 3.25 LMNYY147 pKa = 8.81 VRR149 pKa = 11.84 TKK151 pKa = 10.59 DD152 pKa = 3.42 YY153 pKa = 11.4 TMNEE157 pKa = 5.24 CYY159 pKa = 9.66 TQDD162 pKa = 3.5 SFEE165 pKa = 4.41 AKK167 pKa = 10.55 YY168 pKa = 9.68 NLKK171 pKa = 10.11 WEE173 pKa = 4.69 GSAXRR178 pKa = 11.84 GGVSEE183 pKa = 4.12 KK184 pKa = 10.29 TRR186 pKa = 11.84 VQVVEE191 pKa = 4.31 EE192 pKa = 3.92 LADD195 pKa = 4.99 RR196 pKa = 11.84 IVLADD201 pKa = 4.23 DD202 pKa = 5.0 LGIFPRR208 pKa = 11.84 RR209 pKa = 11.84 GEE211 pKa = 3.86 NGFIIVDD218 pKa = 3.92 EE219 pKa = 4.65 PEE221 pKa = 3.78 VLYY224 pKa = 11.17 AEE226 pKa = 5.11 LEE228 pKa = 4.18 DD229 pKa = 4.95 KK230 pKa = 10.72 ILSFEE235 pKa = 4.11 HH236 pKa = 6.28 SVYY239 pKa = 10.31 HH240 pKa = 5.93 PRR242 pKa = 11.84 AITVEE247 pKa = 4.34 TEE249 pKa = 4.01 VQPLTVKK256 pKa = 9.62 TADD259 pKa = 3.67 SEE261 pKa = 4.56 NLVMMDD267 pKa = 4.27 EE268 pKa = 4.41 EE269 pKa = 4.79 DD270 pKa = 4.07 SIIVTEE276 pKa = 4.04 VLVDD280 pKa = 4.06 DD281 pKa = 4.58 EE282 pKa = 6.04 SDD284 pKa = 4.1 YY285 pKa = 11.4 FWLYY289 pKa = 11.45 LLLLLVSVLISLFILLSRR307 pKa = 11.84 PFILSLGNRR316 pKa = 11.84 RR317 pKa = 11.84 EE318 pKa = 3.99 VFGGDD323 pKa = 2.62 ICYY326 pKa = 10.39 KK327 pKa = 8.7 IAEE330 pKa = 4.51 WLWKK334 pKa = 9.82 VRR336 pKa = 11.84 MWLLKK341 pKa = 9.48 RR342 pKa = 11.84 AMYY345 pKa = 10.43 RR346 pKa = 11.84 FGNLGVVRR354 pKa = 11.84 KK355 pKa = 9.1 NHH357 pKa = 5.91 TKK359 pKa = 10.18 SAMRR363 pKa = 11.84 RR364 pKa = 11.84 YY365 pKa = 9.99 LRR367 pKa = 11.84 DD368 pKa = 3.15 YY369 pKa = 11.06 NFQLEE374 pKa = 4.31 GWEE377 pKa = 4.1 TAINCSPGEE386 pKa = 3.98 ALIAADD392 pKa = 3.29 QHH394 pKa = 6.4 VLVQEE399 pKa = 4.15 VRR401 pKa = 11.84 EE402 pKa = 4.02 LLDD405 pKa = 3.79 LEE407 pKa = 4.7 GNLMSVEE414 pKa = 4.42 KK415 pKa = 10.14 IRR417 pKa = 11.84 KK418 pKa = 8.8 IFEE421 pKa = 4.12 NYY423 pKa = 8.97 NVNNYY428 pKa = 10.2 KK429 pKa = 8.08 ITKK432 pKa = 8.7 WLDD435 pKa = 3.14 KK436 pKa = 10.71 QVIRR440 pKa = 11.84 HH441 pKa = 6.06 ALLAVNEE448 pKa = 4.26 TLKK451 pKa = 10.89 NDD453 pKa = 4.0 EE454 pKa = 4.11 ITLEE458 pKa = 3.95 EE459 pKa = 4.38 GMLNLACATFDD470 pKa = 4.59 QILEE474 pKa = 4.38 CDD476 pKa = 3.56 VEE478 pKa = 4.48 TQRR481 pKa = 11.84 GSMYY485 pKa = 10.59 FGDD488 pKa = 5.58 LIALQFAFRR497 pKa = 11.84 LIGTPEE503 pKa = 3.65 FSDD506 pKa = 3.98 FVVAYY511 pKa = 9.55 KK512 pKa = 11.1 GNLYY516 pKa = 9.45 PCYY519 pKa = 9.75 VEE521 pKa = 4.8 AIKK524 pKa = 10.7 RR525 pKa = 11.84 HH526 pKa = 4.7 ASKK529 pKa = 11.24 LEE531 pKa = 3.9 LQHH534 pKa = 8.41 AEE536 pKa = 3.69 DD537 pKa = 4.95 DD538 pKa = 3.73 DD539 pKa = 4.54 RR540 pKa = 11.84 KK541 pKa = 10.33 RR542 pKa = 11.84 SEE544 pKa = 5.09 KK545 pKa = 10.58 ALEE548 pKa = 3.96 WLLMAGQGAFALSSAMEE565 pKa = 4.32 TFTPAEE571 pKa = 4.2 FQDD574 pKa = 3.45 EE575 pKa = 4.25 MRR577 pKa = 11.84 QVVQAYY583 pKa = 10.4 ADD585 pKa = 4.0 TTFHH589 pKa = 6.38 EE590 pKa = 4.72 ADD592 pKa = 3.92 RR593 pKa = 11.84 PLLSGLGASSIGDD606 pKa = 3.44 VASEE610 pKa = 4.24 FVTGDD615 pKa = 3.01 ISAGTDD621 pKa = 3.26 VEE623 pKa = 4.76 TSSSLSIGTASRR635 pKa = 11.84 ARR637 pKa = 11.84 KK638 pKa = 9.47 RR639 pKa = 11.84 SIARR643 pKa = 11.84 NIRR646 pKa = 11.84 MYY648 pKa = 10.65 PGGAVAHH655 pKa = 6.55 GGVKK659 pKa = 10.23 APRR662 pKa = 11.84 VSGTSGVGGMKK673 pKa = 9.58 MKK675 pKa = 9.59 FTSKK679 pKa = 10.3 PMASS683 pKa = 3.38
Molecular weight: 76.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.137
IPC2_protein 5.169
IPC_protein 5.13
Toseland 5.092
ProMoST 5.27
Dawson 5.118
Bjellqvist 5.245
Wikipedia 4.978
Rodwell 5.054
Grimsley 5.016
Solomon 5.118
Lehninger 5.08
Nozaki 5.245
DTASelect 5.372
Thurlkill 5.092
EMBOSS 5.029
Sillero 5.321
Patrickios 4.164
IPC_peptide 5.13
IPC2_peptide 5.321
IPC2.peptide.svr19 5.304
Protein with the highest isoelectric point:
>tr|Q9QBU6|Q9QBU6_9VIRU Coat protein OS=Oat golden stripe virus OX=45103 GN=cp PE=4 SV=1
MM1 pKa = 7.16 SAAVHH6 pKa = 6.07 SCAKK10 pKa = 10.06 CADD13 pKa = 3.2 GFTRR17 pKa = 11.84 VVCVSKK23 pKa = 10.98 YY24 pKa = 9.91 RR25 pKa = 11.84 NSVYY29 pKa = 10.41 KK30 pKa = 10.2 SLGMEE35 pKa = 4.1 VVKK38 pKa = 10.78 CRR40 pKa = 11.84 LPSACGVNCRR50 pKa = 11.84 MPAALVLVKK59 pKa = 9.35 GHH61 pKa = 7.49 PEE63 pKa = 3.45 LSMDD67 pKa = 4.04 GFCGEE72 pKa = 3.89 KK73 pKa = 9.85 HH74 pKa = 5.94 RR75 pKa = 11.84 GYY77 pKa = 10.45 VVSGAWRR84 pKa = 11.84 HH85 pKa = 4.7 AQLRR89 pKa = 11.84 SLNAEE94 pKa = 3.95 LDD96 pKa = 3.46 KK97 pKa = 10.99 LTEE100 pKa = 4.26 KK101 pKa = 10.91 EE102 pKa = 3.89 EE103 pKa = 4.15 SLRR106 pKa = 11.84 TQIIVLTAAAKK117 pKa = 8.81 TADD120 pKa = 3.47 APVYY124 pKa = 10.38 VPKK127 pKa = 10.62 KK128 pKa = 9.39 LNKK131 pKa = 10.25 LKK133 pKa = 11.17 VEE135 pKa = 4.15 VSDD138 pKa = 3.65 VTEE141 pKa = 4.95 KK142 pKa = 10.28 IHH144 pKa = 6.08 QKK146 pKa = 10.34 SVGLAVVMDD155 pKa = 5.86 AILADD160 pKa = 4.56 LSPDD164 pKa = 3.23 ASPKK168 pKa = 9.82 RR169 pKa = 11.84 RR170 pKa = 11.84 SPAA173 pKa = 3.07
Molecular weight: 18.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.725
IPC2_protein 8.463
IPC_protein 8.375
Toseland 9.019
ProMoST 8.931
Dawson 9.341
Bjellqvist 9.297
Wikipedia 9.414
Rodwell 9.619
Grimsley 9.194
Solomon 9.443
Lehninger 9.443
Nozaki 9.589
DTASelect 9.136
Thurlkill 9.297
EMBOSS 9.531
Sillero 9.502
Patrickios 5.105
IPC_peptide 9.443
IPC2_peptide 8.141
IPC2.peptide.svr19 7.867
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4554
173
1853
759.0
85.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.851 ± 0.722
1.779 ± 0.235
6.588 ± 0.478
7.312 ± 0.436
3.931 ± 0.341
5.336 ± 0.694
1.954 ± 0.17
4.458 ± 0.231
7.246 ± 0.65
9.025 ± 0.199
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.942 ± 0.227
4.414 ± 0.371
3.491 ± 0.274
3.118 ± 0.305
5.797 ± 0.154
6.697 ± 0.355
5.951 ± 0.308
8.608 ± 0.376
1.098 ± 0.109
3.36 ± 0.095
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here