Tomato leaf curl Rajasthan virus - [India:Rajasthan:2005]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato leaf curl Rajasthan virus

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1XCP9|A1XCP9_9GEMI AC4 protein OS=Tomato leaf curl Rajasthan virus - [India:Rajasthan:2005] OX=420254 GN=AC4 PE=3 SV=1
MM1 pKa = 7.64AAPNRR6 pKa = 11.84FKK8 pKa = 10.93INAKK12 pKa = 10.28NYY14 pKa = 7.77FLTYY18 pKa = 9.37PRR20 pKa = 11.84CSLTKK25 pKa = 10.63EE26 pKa = 3.92EE27 pKa = 5.42ALSQLQTLEE36 pKa = 4.24TPTSKK41 pKa = 10.73KK42 pKa = 9.91FIKK45 pKa = 9.76ICRR48 pKa = 11.84EE49 pKa = 3.71LHH51 pKa = 6.27EE52 pKa = 5.93DD53 pKa = 4.19GSPHH57 pKa = 5.9IHH59 pKa = 6.36VLIQFEE65 pKa = 4.89GKK67 pKa = 8.83YY68 pKa = 8.62QCKK71 pKa = 10.05NNRR74 pKa = 11.84FFDD77 pKa = 3.93LVSPSRR83 pKa = 11.84SAHH86 pKa = 4.9FHH88 pKa = 6.45PNIQGAKK95 pKa = 8.99SASDD99 pKa = 3.16VKK101 pKa = 11.13AYY103 pKa = 9.63IDD105 pKa = 3.5KK106 pKa = 11.27DD107 pKa = 3.7GDD109 pKa = 3.7VLEE112 pKa = 4.69WGVFQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.35AYY134 pKa = 10.82AKK136 pKa = 9.97AINMGNKK143 pKa = 9.58DD144 pKa = 3.73DD145 pKa = 4.13ALKK148 pKa = 10.72VLKK151 pKa = 10.24EE152 pKa = 4.25LAPKK156 pKa = 10.39DD157 pKa = 3.72YY158 pKa = 11.2VLQFHH163 pKa = 6.43NLISNLDD170 pKa = 4.25RR171 pKa = 11.84IFQPPSEE178 pKa = 4.67VYY180 pKa = 10.33VSPFSISSFDD190 pKa = 3.37RR191 pKa = 11.84VPPEE195 pKa = 3.62LVDD198 pKa = 3.41WVSSNVVCAAARR210 pKa = 11.84PFRR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84CLGPHH237 pKa = 6.84NYY239 pKa = 10.25LCGHH243 pKa = 7.38LDD245 pKa = 4.39LSPKK249 pKa = 10.12VYY251 pKa = 11.11NNDD254 pKa = 2.08AWYY257 pKa = 10.88NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.47YY287 pKa = 10.28GKK289 pKa = 9.35PVMIKK294 pKa = 10.55GGIPTIFMCNNGPNSSYY311 pKa = 11.09KK312 pKa = 10.48EE313 pKa = 3.93YY314 pKa = 11.01LDD316 pKa = 3.84EE317 pKa = 4.8EE318 pKa = 4.44KK319 pKa = 10.99NAALKK324 pKa = 9.93QWAIKK329 pKa = 9.99NAVFITLEE337 pKa = 3.92EE338 pKa = 3.96PLYY341 pKa = 10.82SGRR344 pKa = 11.84EE345 pKa = 3.92NIAPPEE351 pKa = 3.99EE352 pKa = 4.37EE353 pKa = 4.47EE354 pKa = 4.2EE355 pKa = 4.31HH356 pKa = 6.1SQEE359 pKa = 4.48ASS361 pKa = 3.03

Molecular weight: 40.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1XCP6|A1XCP6_9GEMI Replication enhancer OS=Tomato leaf curl Rajasthan virus - [India:Rajasthan:2005] OX=420254 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.79VSRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 10.71AKK42 pKa = 10.29AWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.87PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.67VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1248

58

361

178.3

20.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.449 ± 0.835

2.163 ± 0.63

5.369 ± 0.362

4.327 ± 0.817

4.087 ± 0.311

4.888 ± 0.284

4.327 ± 0.54

5.929 ± 1.104

6.33 ± 0.557

7.051 ± 1.004

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.515

5.529 ± 0.491

5.529 ± 0.733

4.006 ± 0.777

6.571 ± 0.931

8.013 ± 1.074

5.689 ± 0.904

6.971 ± 0.991

1.603 ± 0.176

4.006 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski