Streptomyces phage Gilgamesh

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 156 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TRA2|A0A5J6TRA2_9CAUD Uncharacterized protein OS=Streptomyces phage Gilgamesh OX=2599890 GN=123 PE=4 SV=1
MM1 pKa = 7.37TATTDD6 pKa = 3.12TAAAPLFDD14 pKa = 4.78EE15 pKa = 4.98NGDD18 pKa = 3.76YY19 pKa = 11.0SPSPGTEE26 pKa = 3.98YY27 pKa = 10.42PFSISDD33 pKa = 3.39IARR36 pKa = 11.84ATARR40 pKa = 11.84LLGKK44 pKa = 10.24GWMAEE49 pKa = 3.52AGYY52 pKa = 9.48WGVTGAVAGPYY63 pKa = 8.53VAEE66 pKa = 4.24FEE68 pKa = 4.89FVVDD72 pKa = 3.6YY73 pKa = 11.38QGDD76 pKa = 3.82LTITYY81 pKa = 10.04SPYY84 pKa = 10.53EE85 pKa = 3.74VDD87 pKa = 4.73GFPEE91 pKa = 4.41VPEE94 pKa = 4.23LPEE97 pKa = 4.12GVKK100 pKa = 10.29EE101 pKa = 4.22CGEE104 pKa = 4.2GVYY107 pKa = 10.92LEE109 pKa = 4.85LACSADD115 pKa = 3.7GLDD118 pKa = 3.84SLAEE122 pKa = 3.87RR123 pKa = 11.84SAAAIRR129 pKa = 11.84AVTGYY134 pKa = 8.84TPDD137 pKa = 3.57AFDD140 pKa = 4.66FEE142 pKa = 5.32SSASRR147 pKa = 11.84QHH149 pKa = 6.87HH150 pKa = 6.99IDD152 pKa = 2.79TGRR155 pKa = 11.84YY156 pKa = 7.77LRR158 pKa = 11.84KK159 pKa = 10.39GEE161 pKa = 4.16ADD163 pKa = 3.31QAA165 pKa = 3.87

Molecular weight: 17.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TR83|A0A5J6TR83_9CAUD ParA-like dsDNA partitioning protein OS=Streptomyces phage Gilgamesh OX=2599890 GN=103 PE=4 SV=1
MM1 pKa = 7.56TSPAPAPTADD11 pKa = 3.94ADD13 pKa = 3.61FHH15 pKa = 6.36AALMSLPYY23 pKa = 9.0EE24 pKa = 4.12QWVPAPIAAARR35 pKa = 11.84VEE37 pKa = 4.59GVGLPRR43 pKa = 11.84EE44 pKa = 4.13ALRR47 pKa = 11.84VVIRR51 pKa = 11.84TGRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84GLLCTKK62 pKa = 10.14RR63 pKa = 11.84DD64 pKa = 3.6PDD66 pKa = 3.36RR67 pKa = 11.84LAFYY71 pKa = 9.97VKK73 pKa = 10.2RR74 pKa = 11.84IAATPRR80 pKa = 11.84TVVPPTAA87 pKa = 4.18

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

156

0

156

38836

41

4624

248.9

27.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.967 ± 0.374

0.883 ± 0.119

6.952 ± 0.472

6.697 ± 0.278

2.189 ± 0.084

7.964 ± 0.279

2.24 ± 0.117

3.214 ± 0.143

2.719 ± 0.188

8.217 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.916 ± 0.089

2.168 ± 0.148

7.478 ± 0.267

3.528 ± 0.151

8.51 ± 0.322

4.717 ± 0.129

6.6 ± 0.156

7.145 ± 0.26

1.823 ± 0.104

2.073 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski