Pseudomonas virus LPB1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Casadabanvirus

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H1ZZC0|H1ZZC0_9CAUD A Transposase OS=Pseudomonas virus LPB1 OX=1091556 PE=4 SV=1
MM1 pKa = 7.67TNTDD5 pKa = 4.59LKK7 pKa = 11.09PLFDD11 pKa = 3.99NLRR14 pKa = 11.84NATEE18 pKa = 4.56FFNSVDD24 pKa = 3.43GDD26 pKa = 3.91LASICNGTKK35 pKa = 10.18QIAAEE40 pKa = 3.99WLEE43 pKa = 4.17TAALALGDD51 pKa = 4.49ALIAQCEE58 pKa = 4.13AVTQEE63 pKa = 4.4DD64 pKa = 4.53SVDD67 pKa = 3.23GRR69 pKa = 11.84PRR71 pKa = 11.84AIEE74 pKa = 4.07CPVCGEE80 pKa = 4.06QEE82 pKa = 4.26LMPGNLCACGYY93 pKa = 7.15EE94 pKa = 4.07TDD96 pKa = 4.09PAAGCACPEE105 pKa = 4.39CDD107 pKa = 4.63GSGDD111 pKa = 3.66GCVGEE116 pKa = 4.47VCRR119 pKa = 11.84EE120 pKa = 3.88CDD122 pKa = 3.24GSGWFVRR129 pKa = 11.84PTAKK133 pKa = 10.34DD134 pKa = 3.19DD135 pKa = 4.48GGDD138 pKa = 3.52HH139 pKa = 6.32EE140 pKa = 4.77

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H1ZZD2|H1ZZD2_9CAUD Putative Mor OS=Pseudomonas virus LPB1 OX=1091556 PE=4 SV=1
MM1 pKa = 7.59SLRR4 pKa = 11.84AVNLAKK10 pKa = 10.31IHH12 pKa = 5.98IAKK15 pKa = 8.12TQLGMDD21 pKa = 4.37DD22 pKa = 3.49DD23 pKa = 4.54TYY25 pKa = 11.18RR26 pKa = 11.84ALLARR31 pKa = 11.84VAGVRR36 pKa = 11.84SAKK39 pKa = 10.42DD40 pKa = 3.08LGPRR44 pKa = 11.84QVGKK48 pKa = 10.77VLAEE52 pKa = 4.0FQRR55 pKa = 11.84LGWKK59 pKa = 9.51PKK61 pKa = 9.59SNRR64 pKa = 11.84QGRR67 pKa = 11.84EE68 pKa = 3.88TPKK71 pKa = 10.23VPQNRR76 pKa = 11.84QTVLRR81 pKa = 11.84KK82 pKa = 8.56ITALLASAHH91 pKa = 6.36RR92 pKa = 11.84PWSYY96 pKa = 10.43ADD98 pKa = 3.09HH99 pKa = 6.39MARR102 pKa = 11.84RR103 pKa = 11.84MFQVEE108 pKa = 4.06RR109 pKa = 11.84VEE111 pKa = 4.2WLDD114 pKa = 4.16DD115 pKa = 3.66SQLYY119 pKa = 10.69RR120 pKa = 11.84LMQALIIDD128 pKa = 4.49RR129 pKa = 11.84SRR131 pKa = 11.84HH132 pKa = 4.02EE133 pKa = 3.81

Molecular weight: 15.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11908

38

1186

220.5

24.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.588 ± 0.59

0.974 ± 0.154

5.979 ± 0.202

5.744 ± 0.27

2.914 ± 0.193

8.003 ± 0.365

1.57 ± 0.154

4.157 ± 0.186

3.502 ± 0.282

9.531 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.14

2.906 ± 0.218

5.039 ± 0.286

5.055 ± 0.35

7.314 ± 0.423

5.761 ± 0.221

5.509 ± 0.255

6.676 ± 0.259

1.806 ± 0.133

2.763 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski