Sclerotinia borealis (strain F-4128)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Sclerotinia; Sclerotinia borealis

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9CD63|W9CD63_SCLBF Uncharacterized protein OS=Sclerotinia borealis (strain F-4128) OX=1432307 GN=SBOR_5861 PE=4 SV=1
MM1 pKa = 7.12NRR3 pKa = 11.84EE4 pKa = 4.26EE5 pKa = 4.61VPSSSDD11 pKa = 3.24PSEE14 pKa = 5.02DD15 pKa = 3.68EE16 pKa = 4.36SSSDD20 pKa = 4.2DD21 pKa = 3.9FDD23 pKa = 4.73ISSQTEE29 pKa = 3.59FDD31 pKa = 3.08NTNRR35 pKa = 11.84QRR37 pKa = 11.84CLEE40 pKa = 3.88EE41 pKa = 4.96RR42 pKa = 11.84YY43 pKa = 9.4PGYY46 pKa = 10.81AGLMLYY52 pKa = 7.96YY53 pKa = 10.15TSQARR58 pKa = 11.84GYY60 pKa = 11.15NYY62 pKa = 10.62GDD64 pKa = 3.76WILSINEE71 pKa = 4.11DD72 pKa = 3.1AGMLQMPQILVIGTT86 pKa = 3.63

Molecular weight: 9.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9CMN6|W9CMN6_SCLBF Uncharacterized protein OS=Sclerotinia borealis (strain F-4128) OX=1432307 GN=SBOR_1593 PE=4 SV=1
MM1 pKa = 7.25SHH3 pKa = 7.44RR4 pKa = 11.84LPHH7 pKa = 6.38HH8 pKa = 6.69LPFLLHH14 pKa = 5.8TLIEE18 pKa = 4.6IPASLNFLLNPSSQLSSPAPQAHH41 pKa = 5.77TLIQQYY47 pKa = 11.02AILLFVSCLIALIFAWRR64 pKa = 11.84PVDD67 pKa = 3.36RR68 pKa = 11.84TARR71 pKa = 11.84LVAGALSLYY80 pKa = 9.62HH81 pKa = 6.63WAPAFRR87 pKa = 11.84AACRR91 pKa = 11.84ILEE94 pKa = 4.21GDD96 pKa = 3.94GVGKK100 pKa = 9.98GVKK103 pKa = 10.07GLGGPGVHH111 pKa = 6.69LLVHH115 pKa = 6.39LLCGAGLLGLFLSGGRR131 pKa = 11.84KK132 pKa = 8.95RR133 pKa = 11.84RR134 pKa = 11.84VGG136 pKa = 3.26

Molecular weight: 14.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10165

0

10165

5168600

39

7261

508.5

56.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.673 ± 0.021

1.136 ± 0.009

5.639 ± 0.017

6.554 ± 0.027

3.662 ± 0.015

6.888 ± 0.025

2.303 ± 0.01

5.494 ± 0.016

5.393 ± 0.023

8.49 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.009

4.189 ± 0.013

5.83 ± 0.022

3.911 ± 0.017

5.738 ± 0.019

8.737 ± 0.033

6.229 ± 0.016

5.755 ± 0.014

1.354 ± 0.009

2.784 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski