Aspergillus lentulus
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9664 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S7DEQ0|A0A0S7DEQ0_9EURO Uncharacterized protein OS=Aspergillus lentulus OX=293939 GN=ALT_0424 PE=3 SV=1
MM1 pKa = 7.39 HH2 pKa = 7.57 LALSLLALSATSTLAAPVNTLPQNQDD28 pKa = 2.49 SNAPTRR34 pKa = 11.84 TARR37 pKa = 11.84 LLTPISHH44 pKa = 7.29 PSKK47 pKa = 10.47 EE48 pKa = 4.11 DD49 pKa = 3.11 SHH51 pKa = 7.9 LKK53 pKa = 10.6 ARR55 pKa = 11.84 TFGLLSGLLSHH66 pKa = 6.81 GFGGSGSAEE75 pKa = 3.9 CEE77 pKa = 3.97 ACEE80 pKa = 4.45 GEE82 pKa = 4.42 AGGSAGGSGGWGGLGFGGHH101 pKa = 6.96 LGGGLGGHH109 pKa = 7.45 LGGGLGGGLGLGGSAGSGEE128 pKa = 4.35 DD129 pKa = 3.5 CDD131 pKa = 4.37 EE132 pKa = 4.47 NGEE135 pKa = 4.37 GGVSVGGSAGGSAGGSAGGSAGGSAGGSAGGSAGGSAGGSAGGSAGGSAGGSLGGSVGGSAGGSAGGSAGGDD207 pKa = 3.25 TGASATGSGSAGGSATGSLGGSASGTAGGSLGGSAGGSAGGSASGTAGGSAGGSATGSLGGSATGSAGGSAGGSAGGDD285 pKa = 3.25 TGASATGSGSGSTGGSATGSVGGSGSVGGSAGGSAGGSAGGSAGGDD331 pKa = 3.25 TGASATGSGSGSTEE345 pKa = 3.8 GSGSTGGSASGSAGGDD361 pKa = 3.15 TGASATGSGSGSTEE375 pKa = 3.71 GSGSGSGSVGGSAGGSAGGSGSSSGGISIGGSATGTGGASASATASASAGASASAGASATGGSGSDD441 pKa = 3.23 NGEE444 pKa = 4.31 DD445 pKa = 4.06 CDD447 pKa = 4.11 EE448 pKa = 4.57 TGADD452 pKa = 3.83 ATTGDD457 pKa = 3.86 NGDD460 pKa = 4.74 DD461 pKa = 3.76 YY462 pKa = 11.87 GNDD465 pKa = 3.38 AGEE468 pKa = 4.94 GDD470 pKa = 4.5 DD471 pKa = 4.89 SSAGGDD477 pKa = 3.52 DD478 pKa = 6.28 SEE480 pKa = 6.76 DD481 pKa = 3.84 CDD483 pKa = 5.05 CDD485 pKa = 3.39 QQ486 pKa = 5.07
Molecular weight: 39.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.656
IPC_protein 3.668
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.617
Rodwell 3.503
Grimsley 3.363
Solomon 3.656
Lehninger 3.617
Nozaki 3.783
DTASelect 4.037
Thurlkill 3.516
EMBOSS 3.63
Sillero 3.795
Patrickios 1.062
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A0S7E3I2|A0A0S7E3I2_9EURO Uncharacterized protein OS=Aspergillus lentulus OX=293939 GN=ALT_7575 PE=4 SV=1
MM1 pKa = 7.4 ASLWRR6 pKa = 11.84 RR7 pKa = 11.84 ILPQRR12 pKa = 11.84 VANSGSQLRR21 pKa = 11.84 DD22 pKa = 3.2 HH23 pKa = 6.56 QANEE27 pKa = 3.99 RR28 pKa = 11.84 TFLSWTRR35 pKa = 11.84 MGLGFAAMALALGRR49 pKa = 11.84 LDD51 pKa = 4.6 AIDD54 pKa = 5.07 RR55 pKa = 11.84 IISSGLSSSKK65 pKa = 11.14 SNLLVVPDD73 pKa = 3.8 NAGAGAAATAGRR85 pKa = 11.84 SGNNINSTEE94 pKa = 3.9 QQPNQISTYY103 pKa = 9.95 LGSGLSAATLCQAISVWSFGYY124 pKa = 10.89 GIFRR128 pKa = 11.84 YY129 pKa = 10.16 LSVRR133 pKa = 11.84 RR134 pKa = 11.84 NLLKK138 pKa = 10.92 GQFTPAIWGPAFITCGCLGVFSSMGLWMEE167 pKa = 3.99 RR168 pKa = 11.84 RR169 pKa = 11.84 DD170 pKa = 4.04 KK171 pKa = 11.23 KK172 pKa = 10.09 MHH174 pKa = 5.87
Molecular weight: 18.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.677
IPC_protein 10.687
Toseland 10.701
ProMoST 10.526
Dawson 10.804
Bjellqvist 10.584
Wikipedia 11.067
Rodwell 10.877
Grimsley 10.862
Solomon 10.979
Lehninger 10.935
Nozaki 10.716
DTASelect 10.57
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.628
IPC_peptide 10.979
IPC2_peptide 9.897
IPC2.peptide.svr19 8.49
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9664
0
9664
5093457
66
6247
527.1
58.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.715 ± 0.024
1.231 ± 0.009
5.617 ± 0.019
6.174 ± 0.026
3.72 ± 0.015
6.782 ± 0.022
2.388 ± 0.01
4.904 ± 0.016
4.599 ± 0.02
9.124 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.114 ± 0.009
3.607 ± 0.012
6.028 ± 0.027
4.031 ± 0.017
6.227 ± 0.02
8.304 ± 0.03
5.88 ± 0.018
6.247 ± 0.017
1.472 ± 0.01
2.837 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here