Caribou feces-associated gemycircularvirus
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A088G061|A0A088G061_9VIRU Cap OS=Caribou feces-associated gemycircularvirus OX=1542743 PE=4 SV=1
MM1 pKa = 7.6 PRR3 pKa = 11.84 FRR5 pKa = 11.84 CRR7 pKa = 11.84 ARR9 pKa = 11.84 NFIITFPQVSEE20 pKa = 4.2 DD21 pKa = 3.42 VQPRR25 pKa = 11.84 FDD27 pKa = 3.87 AGTPFLEE34 pKa = 4.95 RR35 pKa = 11.84 VTADD39 pKa = 3.18 YY40 pKa = 11.21 GNPLCCRR47 pKa = 11.84 LGRR50 pKa = 11.84 EE51 pKa = 3.68 RR52 pKa = 11.84 HH53 pKa = 5.65 ADD55 pKa = 3.35 GGIHH59 pKa = 4.5 YY60 pKa = 9.87 HH61 pKa = 6.4 VYY63 pKa = 10.76 LGFDD67 pKa = 3.95 KK68 pKa = 10.69 IVSINSATAFDD79 pKa = 4.16 YY80 pKa = 11.19 FGAHH84 pKa = 6.18 GNIKK88 pKa = 9.54 SVRR91 pKa = 11.84 RR92 pKa = 11.84 TPNKK96 pKa = 9.45 VFDD99 pKa = 4.09 YY100 pKa = 10.56 VGKK103 pKa = 10.6 DD104 pKa = 2.82 GDD106 pKa = 3.36 IRR108 pKa = 11.84 LEE110 pKa = 4.24 HH111 pKa = 6.79 GEE113 pKa = 4.17 PPAEE117 pKa = 3.98 ARR119 pKa = 11.84 SRR121 pKa = 11.84 DD122 pKa = 3.77 GNDD125 pKa = 2.91 SNKK128 pKa = 8.56 WHH130 pKa = 7.59 DD131 pKa = 4.65 IISCNDD137 pKa = 3.12 KK138 pKa = 11.08 EE139 pKa = 4.63 SFLSAVRR146 pKa = 11.84 EE147 pKa = 3.89 QAPRR151 pKa = 11.84 DD152 pKa = 3.5 WLLSNQRR159 pKa = 11.84 ILEE162 pKa = 4.17 YY163 pKa = 11.19 ANLYY167 pKa = 9.43 YY168 pKa = 10.03 PEE170 pKa = 4.74 EE171 pKa = 4.55 KK172 pKa = 10.11 PEE174 pKa = 3.83 YY175 pKa = 8.23 EE176 pKa = 4.29 APHH179 pKa = 5.18 TTVEE183 pKa = 4.02 RR184 pKa = 11.84 EE185 pKa = 3.86 RR186 pKa = 11.84 YY187 pKa = 9.07 PEE189 pKa = 3.64 ISEE192 pKa = 4.33 WEE194 pKa = 3.98 NQAAIGDD201 pKa = 4.17 SRR203 pKa = 11.84 PEE205 pKa = 3.6 RR206 pKa = 4.62
Molecular weight: 23.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.48
IPC2_protein 5.486
IPC_protein 5.474
Toseland 5.753
ProMoST 5.741
Dawson 5.639
Bjellqvist 5.677
Wikipedia 5.588
Rodwell 5.601
Grimsley 5.855
Solomon 5.639
Lehninger 5.614
Nozaki 5.855
DTASelect 6.02
Thurlkill 5.957
EMBOSS 5.906
Sillero 5.944
Patrickios 4.075
IPC_peptide 5.652
IPC2_peptide 5.957
IPC2.peptide.svr19 5.937
Protein with the highest isoelectric point:
>tr|A0A088G061|A0A088G061_9VIRU Cap OS=Caribou feces-associated gemycircularvirus OX=1542743 PE=4 SV=1
MM1 pKa = 7.84 GYY3 pKa = 10.16 FEE5 pKa = 5.2 EE6 pKa = 4.69 NGYY9 pKa = 10.11 SLSTGLHH16 pKa = 6.2 SSRR19 pKa = 11.84 QHH21 pKa = 7.05 LYY23 pKa = 10.38 RR24 pKa = 11.84 PMAASRR30 pKa = 11.84 FRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 TFRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 STRR43 pKa = 11.84 TPRR46 pKa = 11.84 RR47 pKa = 11.84 YY48 pKa = 8.85 QRR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 SFRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 PRR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 MTSRR65 pKa = 11.84 RR66 pKa = 11.84 VRR68 pKa = 11.84 DD69 pKa = 3.03 IASRR73 pKa = 11.84 KK74 pKa = 9.87 KK75 pKa = 10.05 SDD77 pKa = 3.44 TVFGATTNDD86 pKa = 3.4 VQSTGQANIFAGTNYY101 pKa = 10.0 FIWCPSWRR109 pKa = 11.84 QRR111 pKa = 11.84 RR112 pKa = 11.84 TGSTDD117 pKa = 2.62 EE118 pKa = 4.19 HH119 pKa = 7.24 VRR121 pKa = 11.84 ANQEE125 pKa = 3.54 VYY127 pKa = 9.82 MRR129 pKa = 11.84 GIKK132 pKa = 9.88 DD133 pKa = 3.88 RR134 pKa = 11.84 IMVSATFSLIHH145 pKa = 6.51 RR146 pKa = 11.84 RR147 pKa = 11.84 VCFWSHH153 pKa = 6.1 RR154 pKa = 11.84 RR155 pKa = 11.84 IDD157 pKa = 3.47 DD158 pKa = 3.45 ARR160 pKa = 11.84 PYY162 pKa = 11.12 VFDD165 pKa = 4.24 NPDD168 pKa = 3.51 VIDD171 pKa = 4.04 APQYY175 pKa = 8.93 QRR177 pKa = 11.84 RR178 pKa = 11.84 PLAPIYY184 pKa = 7.42 PTLDD188 pKa = 3.42 SEE190 pKa = 4.12 IFEE193 pKa = 4.45 YY194 pKa = 10.83 LFKK197 pKa = 10.78 GTVGFDD203 pKa = 3.23 YY204 pKa = 11.11 SEE206 pKa = 4.07 NSRR209 pKa = 11.84 WDD211 pKa = 3.44 TPLDD215 pKa = 3.53 NKK217 pKa = 10.08 RR218 pKa = 11.84 VKK220 pKa = 10.23 VVYY223 pKa = 10.04 DD224 pKa = 3.35 KK225 pKa = 11.31 QYY227 pKa = 10.98 TITPNYY233 pKa = 8.64 AAPEE237 pKa = 4.19 GAQFGKK243 pKa = 9.48 TMTRR247 pKa = 11.84 KK248 pKa = 9.27 LWHH251 pKa = 6.67 PINRR255 pKa = 11.84 KK256 pKa = 8.72 ILYY259 pKa = 10.39 DD260 pKa = 4.21 DD261 pKa = 4.76 DD262 pKa = 5.34 EE263 pKa = 5.39 EE264 pKa = 5.35 GADD267 pKa = 3.75 VNGSGWSAQTPDD279 pKa = 3.29 SLGNYY284 pKa = 8.44 YY285 pKa = 10.93 VLDD288 pKa = 3.7 IFSTGQYY295 pKa = 9.91 IEE297 pKa = 4.75 GSTDD301 pKa = 3.28 QVGTYY306 pKa = 8.49 MPEE309 pKa = 3.95 STSYY313 pKa = 8.42 WHH315 pKa = 6.52 EE316 pKa = 3.89 ASS318 pKa = 3.63
Molecular weight: 37.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.701
IPC2_protein 9.575
IPC_protein 10.306
Toseland 9.853
ProMoST 9.823
Dawson 10.189
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.262
Grimsley 10.321
Solomon 10.233
Lehninger 10.16
Nozaki 9.867
DTASelect 9.984
Thurlkill 9.999
EMBOSS 10.306
Sillero 10.101
Patrickios 5.105
IPC_peptide 10.218
IPC2_peptide 8.96
IPC2.peptide.svr19 8.523
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
524
206
318
262.0
30.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.107 ± 0.702
1.145 ± 0.476
7.061 ± 0.132
6.107 ± 1.864
4.771 ± 0.05
6.107 ± 0.122
2.672 ± 0.434
5.153 ± 0.402
3.244 ± 0.092
4.198 ± 0.392
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.527 ± 0.623
4.389 ± 0.568
5.725 ± 0.64
3.435 ± 0.603
12.214 ± 1.208
7.443 ± 0.967
6.107 ± 1.62
4.962 ± 0.064
1.908 ± 0.27
5.725 ± 0.521
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here