Loveridges garter snake virus 1
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A077ETD9|A0A077ETD9_9MONO Phosphoprotein OS=Loveridges garter snake virus 1 OX=1881951 GN=P PE=4 SV=1
MM1 pKa = 7.26 ATRR4 pKa = 11.84 RR5 pKa = 11.84 DD6 pKa = 3.79 NLVSPLEE13 pKa = 4.16 EE14 pKa = 5.05 DD15 pKa = 4.13 PPSTAHH21 pKa = 6.1 RR22 pKa = 11.84 TRR24 pKa = 11.84 SRR26 pKa = 11.84 SPIRR30 pKa = 11.84 RR31 pKa = 11.84 KK32 pKa = 10.11 KK33 pKa = 10.85 SLDD36 pKa = 3.21 KK37 pKa = 10.92 RR38 pKa = 11.84 LLGYY42 pKa = 9.89 SVEE45 pKa = 4.07 EE46 pKa = 4.34 LVDD49 pKa = 3.64 KK50 pKa = 10.97 MSVNPGLIYY59 pKa = 10.75 DD60 pKa = 4.49 KK61 pKa = 10.82 EE62 pKa = 4.05 QLATGRR68 pKa = 11.84 EE69 pKa = 4.13 ALSSEE74 pKa = 4.2 EE75 pKa = 5.0 LIKK78 pKa = 10.87 QLMGEE83 pKa = 4.28 IKK85 pKa = 9.6 TATEE89 pKa = 3.63 EE90 pKa = 3.98 RR91 pKa = 11.84 KK92 pKa = 9.38 IDD94 pKa = 3.59 QEE96 pKa = 4.37 VIQMKK101 pKa = 10.06 FSHH104 pKa = 6.92 LEE106 pKa = 3.89 RR107 pKa = 11.84 TLEE110 pKa = 4.11 SYY112 pKa = 10.8 FEE114 pKa = 4.52 SVVTTQVEE122 pKa = 4.15 ILEE125 pKa = 4.47 KK126 pKa = 10.61 LGQLDD131 pKa = 3.73 YY132 pKa = 11.33 SGSIRR137 pKa = 11.84 QLGEE141 pKa = 3.44 NMKK144 pKa = 10.43 ILDD147 pKa = 4.09 KK148 pKa = 10.98 SLKK151 pKa = 10.43 AVTASVTLMTEE162 pKa = 4.01 KK163 pKa = 10.8 VDD165 pKa = 4.08 LLYY168 pKa = 11.36 GKK170 pKa = 8.97 MAVGTSNAPMIPSCPQPQSIYY191 pKa = 10.14 PKK193 pKa = 10.05 LPEE196 pKa = 4.14 STATAPALDD205 pKa = 3.38 IVFF208 pKa = 4.28
Molecular weight: 23.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.64
IPC2_protein 5.766
IPC_protein 5.715
Toseland 5.855
ProMoST 5.881
Dawson 5.779
Bjellqvist 5.855
Wikipedia 5.69
Rodwell 5.741
Grimsley 5.881
Solomon 5.792
Lehninger 5.753
Nozaki 5.957
DTASelect 6.097
Thurlkill 5.97
EMBOSS 5.906
Sillero 6.059
Patrickios 4.584
IPC_peptide 5.804
IPC2_peptide 6.02
IPC2.peptide.svr19 6.249
Protein with the highest isoelectric point:
>tr|A0A077ETD9|A0A077ETD9_9MONO Phosphoprotein OS=Loveridges garter snake virus 1 OX=1881951 GN=P PE=4 SV=1
MM1 pKa = 7.48 APKK4 pKa = 10.17 HH5 pKa = 5.99 EE6 pKa = 4.44 YY7 pKa = 9.98 IEE9 pKa = 5.28 LKK11 pKa = 10.81 DD12 pKa = 3.77 KK13 pKa = 11.33 VIVPGWKK20 pKa = 8.38 TLMLEE25 pKa = 3.68 IDD27 pKa = 4.08 FVGGASRR34 pKa = 11.84 NQFVNFPLLSVKK46 pKa = 10.68 DD47 pKa = 3.94 DD48 pKa = 3.59 FRR50 pKa = 11.84 LPRR53 pKa = 11.84 EE54 pKa = 3.84 KK55 pKa = 10.71 RR56 pKa = 11.84 LINYY60 pKa = 7.48 LTVDD64 pKa = 4.45 AEE66 pKa = 4.39 PSGHH70 pKa = 6.04 GSVNIYY76 pKa = 10.99 LLFSDD81 pKa = 5.88 FLVQTLNSISVYY93 pKa = 10.34 KK94 pKa = 10.78 DD95 pKa = 3.26 PIKK98 pKa = 9.78 QHH100 pKa = 5.18 MYY102 pKa = 10.38 VRR104 pKa = 11.84 LTEE107 pKa = 4.22 NQSKK111 pKa = 9.91 HH112 pKa = 6.19 AINAAFDD119 pKa = 3.43 VHH121 pKa = 7.17 SYY123 pKa = 10.54 RR124 pKa = 11.84 LRR126 pKa = 11.84 NVGVGPLGPDD136 pKa = 2.9 IRR138 pKa = 11.84 ATSPP142 pKa = 2.9
Molecular weight: 16.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.361
IPC2_protein 8.302
IPC_protein 8.448
Toseland 8.96
ProMoST 8.785
Dawson 9.194
Bjellqvist 8.858
Wikipedia 9.385
Rodwell 9.341
Grimsley 9.268
Solomon 9.385
Lehninger 9.341
Nozaki 8.814
DTASelect 8.902
Thurlkill 9.004
EMBOSS 9.326
Sillero 9.107
Patrickios 4.673
IPC_peptide 9.37
IPC2_peptide 7.585
IPC2.peptide.svr19 7.583
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3028
89
1713
504.7
56.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.209 ± 0.8
1.948 ± 0.527
5.02 ± 0.159
5.746 ± 0.826
3.732 ± 0.41
5.68 ± 0.538
2.048 ± 0.24
6.968 ± 0.574
5.35 ± 0.342
10.766 ± 0.521
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.543 ± 0.272
3.666 ± 0.77
5.218 ± 0.299
3.369 ± 0.4
5.68 ± 0.307
8.818 ± 0.611
6.011 ± 0.427
6.341 ± 0.631
1.387 ± 0.322
3.501 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here