California sea lion polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus; Zalophus californianus polyomavirus 1

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3IZT2|D3IZT2_9POLY Minor capsid protein OS=California sea lion polyomavirus 1 OX=715223 PE=3 SV=1
MM1 pKa = 7.46GALISVITEE10 pKa = 4.18LFGLAAATGFTTEE23 pKa = 5.09AILSGEE29 pKa = 3.79AAAAIEE35 pKa = 4.13GLLDD39 pKa = 3.36EE40 pKa = 4.51VAYY43 pKa = 10.82VSLVDD48 pKa = 5.4GVTDD52 pKa = 4.14LQALEE57 pKa = 4.24SFGISEE63 pKa = 4.44EE64 pKa = 4.16LFNLYY69 pKa = 10.66LDD71 pKa = 3.99IPRR74 pKa = 11.84GASEE78 pKa = 4.14VLASLGEE85 pKa = 3.96AAKK88 pKa = 10.78GSIGEE93 pKa = 4.24FGVLALSIAGASLSPLSYY111 pKa = 9.23TQAVPDD117 pKa = 3.61MALQVWVPDD126 pKa = 3.15VDD128 pKa = 3.94YY129 pKa = 11.02LFPGVSTFARR139 pKa = 11.84YY140 pKa = 8.33AQYY143 pKa = 11.25LDD145 pKa = 3.41PTYY148 pKa = 10.26WGPDD152 pKa = 3.41LLNHH156 pKa = 6.21VSRR159 pKa = 11.84LFWQSLLSEE168 pKa = 4.25GRR170 pKa = 11.84RR171 pKa = 11.84QIGHH175 pKa = 7.74ASRR178 pKa = 11.84DD179 pKa = 3.29LATTVGGTVQEE190 pKa = 4.18SLARR194 pKa = 11.84FFEE197 pKa = 4.13NTRR200 pKa = 11.84WAVSHH205 pKa = 5.83VTGDD209 pKa = 3.22WYY211 pKa = 10.94AALHH215 pKa = 6.56RR216 pKa = 11.84YY217 pKa = 9.22YY218 pKa = 11.33GEE220 pKa = 4.68LPPLNPPQLSQLRR233 pKa = 11.84KK234 pKa = 9.17RR235 pKa = 11.84IQRR238 pKa = 11.84EE239 pKa = 3.66TGIDD243 pKa = 3.31VAEE246 pKa = 4.31EE247 pKa = 4.31DD248 pKa = 3.63KK249 pKa = 11.25SHH251 pKa = 6.82RR252 pKa = 11.84PSANFVQKK260 pKa = 10.12YY261 pKa = 9.15SPPGGAGQRR270 pKa = 11.84HH271 pKa = 5.43APDD274 pKa = 3.09WLLPLLLGLYY284 pKa = 10.72GDD286 pKa = 5.42LTPTWKK292 pKa = 10.69KK293 pKa = 9.72EE294 pKa = 3.91VEE296 pKa = 4.07EE297 pKa = 4.35LEE299 pKa = 4.44EE300 pKa = 5.49IEE302 pKa = 4.72TEE304 pKa = 4.28DD305 pKa = 5.24EE306 pKa = 3.66EE307 pKa = 6.1DD308 pKa = 3.62IPKK311 pKa = 10.34KK312 pKa = 9.69KK313 pKa = 9.78QRR315 pKa = 11.84SASLPGAKK323 pKa = 8.95TPHH326 pKa = 6.31KK327 pKa = 10.08RR328 pKa = 11.84RR329 pKa = 11.84NRR331 pKa = 11.84SVRR334 pKa = 11.84RR335 pKa = 11.84PNRR338 pKa = 11.84RR339 pKa = 11.84RR340 pKa = 3.25

Molecular weight: 37.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3IZT1|D3IZT1_9POLY Minor capsid protein OS=California sea lion polyomavirus 1 OX=715223 PE=3 SV=1
MM1 pKa = 7.58KK2 pKa = 10.39KK3 pKa = 9.59ILRR6 pKa = 11.84LALCPDD12 pKa = 4.31GVRR15 pKa = 11.84LPACPGFAYY24 pKa = 9.47WAVAPPSAGPFGPQPLLLLTRR45 pKa = 11.84GAQPKK50 pKa = 9.72

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1956

50

662

326.0

36.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.47 ± 1.046

1.84 ± 0.588

5.828 ± 0.504

7.413 ± 0.34

3.476 ± 0.196

7.311 ± 0.859

2.301 ± 0.196

4.039 ± 0.412

6.493 ± 0.944

11.043 ± 0.624

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.892 ± 0.384

3.732 ± 0.467

6.697 ± 1.399

3.885 ± 0.281

5.93 ± 0.721

6.595 ± 0.251

5.061 ± 0.676

5.521 ± 0.634

1.738 ± 0.477

3.732 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski