Bhendi yellow vein mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3HIP4|D3HIP4_9GEMI Replication-associated protein OS=Bhendi yellow vein mosaic virus OX=120168 GN=AC1 PE=3 SV=1
MM1 pKa = 7.43PSKK4 pKa = 10.64RR5 pKa = 11.84FQIYY9 pKa = 9.59SKK11 pKa = 10.96NYY13 pKa = 7.65FLTYY17 pKa = 9.19PKK19 pKa = 10.53CSLTKK24 pKa = 10.66EE25 pKa = 4.11EE26 pKa = 5.14ALSQIQNLHH35 pKa = 5.57TPTNKK40 pKa = 9.89KK41 pKa = 9.76FIKK44 pKa = 9.65ICRR47 pKa = 11.84EE48 pKa = 3.52LHH50 pKa = 6.26EE51 pKa = 4.93NGEE54 pKa = 4.28PHH56 pKa = 6.66LHH58 pKa = 6.22VLIQFDD64 pKa = 4.81GKK66 pKa = 10.33YY67 pKa = 9.72KK68 pKa = 10.36CQNQRR73 pKa = 11.84FFDD76 pKa = 3.97LVSPSRR82 pKa = 11.84SVNFHH87 pKa = 6.81PNIQGAKK94 pKa = 9.25SSSDD98 pKa = 3.11VKK100 pKa = 11.24SYY102 pKa = 10.79IDD104 pKa = 3.7KK105 pKa = 11.33DD106 pKa = 3.49GDD108 pKa = 3.67TLEE111 pKa = 4.31WGGSKK116 pKa = 10.25SEE118 pKa = 3.88ALRR121 pKa = 11.84VIRR124 pKa = 11.84EE125 pKa = 4.33LAPKK129 pKa = 10.23DD130 pKa = 3.59YY131 pKa = 11.19VLQFHH136 pKa = 6.76NLNANLDD143 pKa = 4.3RR144 pKa = 11.84IFTPPLEE151 pKa = 4.56VYY153 pKa = 10.35VSPFLSSSFDD163 pKa = 3.44QVPEE167 pKa = 3.95EE168 pKa = 4.04LEE170 pKa = 3.96EE171 pKa = 4.22WVSEE175 pKa = 4.09NVMDD179 pKa = 5.26AAARR183 pKa = 11.84PLRR186 pKa = 11.84PLSLVLEE193 pKa = 4.7GDD195 pKa = 3.46SRR197 pKa = 11.84TGKK200 pKa = 8.52TMWARR205 pKa = 11.84SLGPHH210 pKa = 6.75NYY212 pKa = 10.24LCGNLDD218 pKa = 4.48LSPKK222 pKa = 10.02VYY224 pKa = 11.09SNDD227 pKa = 2.39AWFNIIDD234 pKa = 4.53DD235 pKa = 4.12VDD237 pKa = 3.5PHH239 pKa = 5.85YY240 pKa = 11.0LKK242 pKa = 10.7HH243 pKa = 6.2FKK245 pKa = 10.7EE246 pKa = 4.46FMGAQRR252 pKa = 11.84DD253 pKa = 3.81WQSNTKK259 pKa = 9.6YY260 pKa = 10.57GKK262 pKa = 9.12PVQIKK267 pKa = 10.41GGIPTIFLCNPGPNASYY284 pKa = 11.17KK285 pKa = 10.64EE286 pKa = 3.98FLDD289 pKa = 3.76EE290 pKa = 4.66EE291 pKa = 4.6KK292 pKa = 11.17NSALKK297 pKa = 10.45AWALKK302 pKa = 9.77NAAFVFLTEE311 pKa = 4.27PLYY314 pKa = 11.31SGTNQSTAQGSEE326 pKa = 3.87EE327 pKa = 3.99THH329 pKa = 5.98QEE331 pKa = 3.91EE332 pKa = 4.9EE333 pKa = 4.36SRR335 pKa = 11.84SS336 pKa = 3.67

Molecular weight: 38.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3HIP1|D3HIP1_9GEMI Capsid protein OS=Bhendi yellow vein mosaic virus OX=120168 GN=AV1 PE=3 SV=1
MM1 pKa = 7.46ILVFSCLLLVVNNIIVNPNKK21 pKa = 10.13FLNQRR26 pKa = 11.84LFLTRR31 pKa = 11.84ILSTSNGCMPFPQHH45 pKa = 6.51LVPIPMHH52 pKa = 5.53VLHH55 pKa = 6.72GRR57 pKa = 11.84RR58 pKa = 11.84TGLVIKK64 pKa = 9.76HH65 pKa = 5.92IKK67 pKa = 10.34YY68 pKa = 7.17FTKK71 pKa = 10.2ILWFICRR78 pKa = 11.84SSITNKK84 pKa = 9.71EE85 pKa = 3.56KK86 pKa = 9.65HH87 pKa = 5.68HH88 pKa = 7.13RR89 pKa = 11.84IRR91 pKa = 11.84MVLGLDD97 pKa = 3.84VLVHH101 pKa = 6.77PYY103 pKa = 8.71LTT105 pKa = 3.88

Molecular weight: 12.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1260

105

336

180.0

20.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.286 ± 0.814

2.46 ± 0.597

4.444 ± 0.374

4.286 ± 0.954

4.603 ± 0.398

4.683 ± 0.216

3.968 ± 0.545

5.159 ± 0.79

5.794 ± 0.717

8.333 ± 1.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.523

6.587 ± 0.623

5.952 ± 0.509

4.444 ± 0.675

6.905 ± 0.991

7.46 ± 1.117

5.952 ± 0.703

6.746 ± 0.983

1.349 ± 0.13

4.286 ± 0.465
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski