Streptomyces phage Alvy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TRH4|A0A5J6TRH4_9CAUD Membrane protein OS=Streptomyces phage Alvy OX=2599888 GN=29 PE=4 SV=1
MM1 pKa = 7.75ALNDD5 pKa = 3.72AATLVIGSGNYY16 pKa = 7.79LTAPVGTDD24 pKa = 2.71IPTDD28 pKa = 3.63LLVPVSPWEE37 pKa = 4.27AVGHH41 pKa = 5.85TSLEE45 pKa = 4.62DD46 pKa = 3.09IFSIASEE53 pKa = 4.21GGEE56 pKa = 3.82ATVIGSLQNKK66 pKa = 7.32SLRR69 pKa = 11.84TKK71 pKa = 10.19YY72 pKa = 10.01SARR75 pKa = 11.84TEE77 pKa = 4.14TMAFTLQQFDD87 pKa = 3.93VKK89 pKa = 10.56GLRR92 pKa = 11.84LYY94 pKa = 10.58YY95 pKa = 10.58GSNAPILPDD104 pKa = 3.49GSVGVPADD112 pKa = 3.93PVPTTAAFLAVFVDD126 pKa = 4.2GEE128 pKa = 4.59NVFAFYY134 pKa = 10.79APKK137 pKa = 10.81AEE139 pKa = 4.35IYY141 pKa = 10.76RR142 pKa = 11.84NDD144 pKa = 3.94DD145 pKa = 3.38LSFGDD150 pKa = 3.96TEE152 pKa = 4.59SLAGLPLGVKK162 pKa = 10.0PMTYY166 pKa = 10.41GANSWTYY173 pKa = 11.09AITPLGTVSATGATAGTPGTFTPAGSTAPYY203 pKa = 10.82DD204 pKa = 4.58LVDD207 pKa = 4.94LDD209 pKa = 5.83LVTADD214 pKa = 4.91PATAWTTGQYY224 pKa = 10.36VVLGDD229 pKa = 4.16GSNAYY234 pKa = 9.03WNGTDD239 pKa = 2.94WVTGIAAA246 pKa = 4.35

Molecular weight: 25.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TR20|A0A5J6TR20_9CAUD Uncharacterized protein OS=Streptomyces phage Alvy OX=2599888 GN=88 PE=4 SV=1
MM1 pKa = 7.55PNWEE5 pKa = 4.02GSDD8 pKa = 3.26RR9 pKa = 11.84RR10 pKa = 11.84SRR12 pKa = 11.84LPKK15 pKa = 9.76DD16 pKa = 3.0WPKK19 pKa = 10.43IRR21 pKa = 11.84LRR23 pKa = 11.84VLRR26 pKa = 11.84RR27 pKa = 11.84DD28 pKa = 3.54GGQCTALTEE37 pKa = 4.34AGEE40 pKa = 4.48RR41 pKa = 11.84CDD43 pKa = 5.01SLASDD48 pKa = 4.15VDD50 pKa = 4.3HH51 pKa = 7.06IRR53 pKa = 11.84PGDD56 pKa = 3.7DD57 pKa = 3.06HH58 pKa = 8.57SLANLRR64 pKa = 11.84SLCSWHH70 pKa = 6.68HH71 pKa = 5.1RR72 pKa = 11.84QKK74 pKa = 10.9SSRR77 pKa = 11.84EE78 pKa = 3.88GAEE81 pKa = 3.54AAHH84 pKa = 6.15ARR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84AIQKK92 pKa = 9.32RR93 pKa = 11.84FKK95 pKa = 9.94RR96 pKa = 11.84TEE98 pKa = 3.65AHH100 pKa = 6.86PGLLL104 pKa = 3.89

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

16369

24

1099

183.9

20.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.375 ± 0.329

0.794 ± 0.124

6.457 ± 0.227

6.952 ± 0.355

2.902 ± 0.233

8.369 ± 0.305

2.077 ± 0.224

4.093 ± 0.271

4.46 ± 0.242

8.369 ± 0.357

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.125

2.749 ± 0.153

5.052 ± 0.21

3.274 ± 0.158

6.744 ± 0.47

5.773 ± 0.224

6.36 ± 0.241

7.288 ± 0.272

1.912 ± 0.11

2.865 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski