Candidatus Micrarchaeum acidiphilum ARMAN-2

Taxonomy: cellular organisms; Archaea; DPANN group; Candidatus Micrarchaeota; Candidatus Micrarchaeum; Candidatus Micrarchaeum acidiphilum

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1025 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7DGU6|C7DGU6_MICA2 Uncharacterized protein OS=Candidatus Micrarchaeum acidiphilum ARMAN-2 OX=425595 GN=UNLARM2_0296 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.17AYY4 pKa = 9.68IYY6 pKa = 10.95GSMIAVGLAIGIFGAVLMLGFGFAQGTGTVTANVAVGNVIYY47 pKa = 10.37LAISPNSIVISGLAPNSIYY66 pKa = 8.58PTNIPVTDD74 pKa = 3.74TDD76 pKa = 3.32NGGNIGANALVEE88 pKa = 4.13GTNWVNQTYY97 pKa = 8.22TFGVSNTLEE106 pKa = 4.39SNTASATTLSGTPLSNSFAVTNSYY130 pKa = 10.4IIAPNVVNPSTNTIIYY146 pKa = 9.23FGFSIPPATPAGNYY160 pKa = 8.69LQTISFEE167 pKa = 4.24NEE169 pKa = 3.47NVSNRR174 pKa = 11.84EE175 pKa = 3.51FNAISTSNSVTITANVIGTCYY196 pKa = 10.25ISLSPNSIDD205 pKa = 5.21FGTLTPSANVPTNVGVNDD223 pKa = 3.69IDD225 pKa = 3.82NGGNVAAQLFVYY237 pKa = 9.52GGNWIGPANFEE248 pKa = 4.3VSNTTWSAASGTSFGTAARR267 pKa = 11.84LTGAANDD274 pKa = 3.98TNIVIPAPTPSNAVTSNSIYY294 pKa = 10.29FGLGIPPGTPAGTYY308 pKa = 9.45LQTITIEE315 pKa = 4.19NSCC318 pKa = 4.15

Molecular weight: 32.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7DGN4|C7DGN4_MICA2 DNA polymerase II large subunit OS=Candidatus Micrarchaeum acidiphilum ARMAN-2 OX=425595 GN=polC PE=3 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84EE3 pKa = 3.68NKK5 pKa = 9.41LRR7 pKa = 11.84RR8 pKa = 11.84SSNRR12 pKa = 11.84QILATLEE19 pKa = 4.57GYY21 pKa = 10.76GLTEE25 pKa = 4.08FQTAVLKK32 pKa = 9.83ATLSIPKK39 pKa = 10.34GEE41 pKa = 4.01TRR43 pKa = 11.84TYY45 pKa = 10.25KK46 pKa = 10.57QIASLVGRR54 pKa = 11.84PRR56 pKa = 11.84AYY58 pKa = 10.08RR59 pKa = 11.84AVGTALKK66 pKa = 10.43NNPLPIIIPCHH77 pKa = 4.97RR78 pKa = 11.84VIRR81 pKa = 11.84SDD83 pKa = 4.29GEE85 pKa = 4.07TGNYY89 pKa = 9.94NGTGRR94 pKa = 11.84SGRR97 pKa = 11.84LKK99 pKa = 10.46KK100 pKa = 10.38IKK102 pKa = 9.61LLKK105 pKa = 10.06MEE107 pKa = 4.58GALL110 pKa = 4.29

Molecular weight: 12.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1025

0

1025

303770

38

2034

296.4

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.431 ± 0.074

0.79 ± 0.031

4.719 ± 0.079

6.141 ± 0.115

4.309 ± 0.064

7.449 ± 0.077

1.475 ± 0.028

8.126 ± 0.075

7.296 ± 0.114

8.806 ± 0.092

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.038

4.663 ± 0.082

3.624 ± 0.052

2.413 ± 0.04

4.613 ± 0.081

7.885 ± 0.101

4.952 ± 0.118

6.631 ± 0.06

0.725 ± 0.023

4.14 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski