Corynebacterium lowii
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2252 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q0UIN0|A0A0Q0UIN0_9CORY N-acetylmuramoyl-L-alanine amidase OS=Corynebacterium lowii OX=1544413 GN=Clow_01493 PE=3 SV=1
MM1 pKa = 7.52 SLRR4 pKa = 11.84 FRR6 pKa = 11.84 TAATLAASALVTVGVATATAEE27 pKa = 3.74 EE28 pKa = 4.32 SLPVEE33 pKa = 4.12 GALPVEE39 pKa = 4.15 GTTWNFQGSSSAYY52 pKa = 7.93 FTLNDD57 pKa = 3.96 GNIGGNDD64 pKa = 3.34 GCNAFGGSATLEE76 pKa = 4.32 GDD78 pKa = 3.59 TLTVGDD84 pKa = 4.95 LFSTMRR90 pKa = 11.84 YY91 pKa = 9.86 CGDD94 pKa = 3.4 GYY96 pKa = 11.4 EE97 pKa = 4.48 LTTALAGEE105 pKa = 4.47 HH106 pKa = 4.69 TVEE109 pKa = 4.27 VQGDD113 pKa = 4.02 TLTLRR118 pKa = 11.84 DD119 pKa = 3.86 EE120 pKa = 5.89 NGTEE124 pKa = 3.98 WNFVAAEE131 pKa = 3.96
Molecular weight: 13.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.859
IPC_protein 3.77
Toseland 3.592
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.961
Wikipedia 3.643
Rodwell 3.605
Grimsley 3.503
Solomon 3.719
Lehninger 3.668
Nozaki 3.872
DTASelect 3.999
Thurlkill 3.63
EMBOSS 3.656
Sillero 3.884
Patrickios 1.85
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A0Q1DX62|A0A0Q1DX62_9CORY Trehalose import ATP-binding protein SugC OS=Corynebacterium lowii OX=1544413 GN=sugC PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.39 KK16 pKa = 9.04 HH17 pKa = 5.39 RR18 pKa = 11.84 KK19 pKa = 5.37 MLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2252
0
2252
707702
31
2178
314.3
33.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.067 ± 0.07
0.73 ± 0.014
5.217 ± 0.043
6.834 ± 0.062
3.084 ± 0.035
8.59 ± 0.046
2.192 ± 0.026
4.65 ± 0.039
2.756 ± 0.046
10.124 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.242 ± 0.027
2.512 ± 0.028
5.243 ± 0.045
3.401 ± 0.034
6.646 ± 0.061
5.961 ± 0.036
5.837 ± 0.038
8.224 ± 0.043
1.438 ± 0.023
2.253 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here