Flavobacterium terrae
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2883 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6FQ51|A0A1M6FQ51_9FLAO Lipopolysaccharide export system ATP-binding protein OS=Flavobacterium terrae OX=415425 GN=SAMN05444363_2283 PE=4 SV=1
MM1 pKa = 6.41 KK2 pKa = 10.16 QKK4 pKa = 10.8 YY5 pKa = 9.02 ILFIIFSLLVFKK17 pKa = 11.06 GKK19 pKa = 9.92 AQNNYY24 pKa = 8.9 SVAPIPFQPFIGSLTPLNALDD45 pKa = 5.14 DD46 pKa = 4.29 MYY48 pKa = 11.78 SNIIPLPFSFDD59 pKa = 4.04 FYY61 pKa = 11.46 GVTYY65 pKa = 10.67 NQMIVSTNGFINFTTTSAGGVSPWSFSQQIPNASFPVTNSILGCFEE111 pKa = 5.08 DD112 pKa = 5.18 LYY114 pKa = 11.03 NNSGTGSITYY124 pKa = 9.47 GVYY127 pKa = 9.2 GTAPYY132 pKa = 10.43 RR133 pKa = 11.84 KK134 pKa = 8.52 FVVYY138 pKa = 10.45 FNDD141 pKa = 3.63 QPHH144 pKa = 6.42 FSCNTIAISSFQMILSEE161 pKa = 4.14 TANTIDD167 pKa = 3.15 VQLIEE172 pKa = 4.5 RR173 pKa = 11.84 QPCLGWNSGRR183 pKa = 11.84 GVMGIVKK190 pKa = 10.46 DD191 pKa = 3.87 GANALAAPGRR201 pKa = 11.84 NTGSWTANNEE211 pKa = 3.05 AWRR214 pKa = 11.84 FYY216 pKa = 10.77 RR217 pKa = 11.84 AGYY220 pKa = 8.34 YY221 pKa = 9.83 PNYY224 pKa = 10.6 SFVRR228 pKa = 11.84 CDD230 pKa = 4.34 DD231 pKa = 3.86 DD232 pKa = 3.68 TDD234 pKa = 4.36 GYY236 pKa = 11.0 QVFNLSVAANDD247 pKa = 4.05 LVPANPSSVIFYY259 pKa = 8.42 STLTDD264 pKa = 3.4 AQADD268 pKa = 4.16 TNSITNQTAYY278 pKa = 9.62 TNNSNPQIIYY288 pKa = 10.11 AKK290 pKa = 9.74 EE291 pKa = 3.45 NGIIKK296 pKa = 9.01 TVTLSVFDD304 pKa = 4.97 CNVDD308 pKa = 3.22 ADD310 pKa = 4.05 NDD312 pKa = 4.34 GVSSSTEE319 pKa = 3.71 DD320 pKa = 3.37 VNNDD324 pKa = 2.91 TNLANDD330 pKa = 3.81 DD331 pKa = 4.09 TDD333 pKa = 4.78 FDD335 pKa = 5.31 GIPNYY340 pKa = 10.52 LDD342 pKa = 4.06 NDD344 pKa = 4.18 DD345 pKa = 6.02 DD346 pKa = 5.91 GDD348 pKa = 4.13 LVLTNIEE355 pKa = 4.27 YY356 pKa = 10.91 VFGKK360 pKa = 10.09 NVSALLDD367 pKa = 3.7 TDD369 pKa = 3.61 NDD371 pKa = 4.91 GIPNYY376 pKa = 10.35 LDD378 pKa = 4.14 NDD380 pKa = 4.21 DD381 pKa = 5.78 DD382 pKa = 6.08 GDD384 pKa = 3.99 GSLTFLEE391 pKa = 5.53 DD392 pKa = 3.51 YY393 pKa = 10.9 NHH395 pKa = 7.61 DD396 pKa = 4.1 GNPVNDD402 pKa = 3.65 DD403 pKa = 3.78 TNFNNIPDD411 pKa = 4.24 YY412 pKa = 11.17 LDD414 pKa = 3.2 QAVTLGVSQVRR425 pKa = 11.84 IDD427 pKa = 3.92 DD428 pKa = 4.09 NAILMYY434 pKa = 9.7 PNPATSMLNILNKK447 pKa = 7.52 TTEE450 pKa = 4.03 NVMLIEE456 pKa = 5.04 IYY458 pKa = 10.61 NSNGSRR464 pKa = 11.84 AKK466 pKa = 10.46 SINSTQSLTTIPVSDD481 pKa = 4.34 LQSGVYY487 pKa = 8.29 FVKK490 pKa = 10.77 VIMNNQVVNYY500 pKa = 10.15 KK501 pKa = 10.28 FIKK504 pKa = 10.38 DD505 pKa = 3.28
Molecular weight: 55.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.795
IPC_protein 3.846
Toseland 3.605
ProMoST 4.012
Dawson 3.859
Bjellqvist 4.037
Wikipedia 3.834
Rodwell 3.668
Grimsley 3.503
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.291
Thurlkill 3.668
EMBOSS 3.834
Sillero 3.973
Patrickios 0.515
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.877
Protein with the highest isoelectric point:
>tr|A0A1M6BEJ7|A0A1M6BEJ7_9FLAO NADH-quinone oxidoreductase subunit N OS=Flavobacterium terrae OX=415425 GN=nuoN PE=3 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.35 KK16 pKa = 10.31 RR17 pKa = 11.84 KK18 pKa = 9.82 KK19 pKa = 8.65 RR20 pKa = 11.84 ARR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.91 KK27 pKa = 10.59 KK28 pKa = 9.82 KK29 pKa = 10.51 KK30 pKa = 10.04
Molecular weight: 3.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.023
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.705
Grimsley 12.764
Solomon 13.217
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.427
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.989
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2883
0
2883
969809
29
3322
336.4
38.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.065 ± 0.058
0.811 ± 0.016
5.282 ± 0.035
6.572 ± 0.056
5.461 ± 0.04
6.184 ± 0.049
1.644 ± 0.022
8.114 ± 0.052
8.33 ± 0.069
8.996 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.187 ± 0.027
6.726 ± 0.053
3.282 ± 0.031
3.423 ± 0.027
3.02 ± 0.029
6.706 ± 0.041
5.817 ± 0.067
6.257 ± 0.038
0.977 ± 0.019
4.147 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here