Amino acid dipepetide frequency for Klebsiella phage KP8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.399AlaAla: 8.399 ± 1.3
0.612AlaCys: 0.612 ± 0.18
4.899AlaAsp: 4.899 ± 0.499
5.862AlaGlu: 5.862 ± 0.622
2.668AlaPhe: 2.668 ± 0.458
5.949AlaGly: 5.949 ± 0.553
1.137AlaHis: 1.137 ± 0.196
4.243AlaIle: 4.243 ± 0.462
5.468AlaLys: 5.468 ± 0.54
8.005AlaLeu: 8.005 ± 0.807
2.406AlaMet: 2.406 ± 0.442
4.068AlaAsn: 4.068 ± 0.477
3.018AlaPro: 3.018 ± 0.387
4.024AlaGln: 4.024 ± 0.745
3.674AlaArg: 3.674 ± 0.422
5.337AlaSer: 5.337 ± 0.585
5.468AlaThr: 5.468 ± 0.544
5.337AlaVal: 5.337 ± 0.662
0.962AlaTrp: 0.962 ± 0.223
3.499AlaTyr: 3.499 ± 0.401
0.0AlaXaa: 0.0 ± 0.0
Cys
0.7CysAla: 0.7 ± 0.186
0.087CysCys: 0.087 ± 0.061
0.481CysAsp: 0.481 ± 0.142
0.262CysGlu: 0.262 ± 0.118
0.612CysPhe: 0.612 ± 0.175
0.787CysGly: 0.787 ± 0.222
0.219CysHis: 0.219 ± 0.098
0.569CysIle: 0.569 ± 0.163
0.7CysLys: 0.7 ± 0.199
0.569CysLeu: 0.569 ± 0.177
0.219CysMet: 0.219 ± 0.114
0.787CysAsn: 0.787 ± 0.259
0.394CysPro: 0.394 ± 0.184
0.087CysGln: 0.087 ± 0.065
0.437CysArg: 0.437 ± 0.145
0.437CysSer: 0.437 ± 0.131
0.656CysThr: 0.656 ± 0.212
0.394CysVal: 0.394 ± 0.151
0.175CysTrp: 0.175 ± 0.098
0.481CysTyr: 0.481 ± 0.168
0.0CysXaa: 0.0 ± 0.0
Asp
5.249AspAla: 5.249 ± 0.678
0.525AspCys: 0.525 ± 0.154
3.412AspAsp: 3.412 ± 0.511
3.806AspGlu: 3.806 ± 0.443
2.406AspPhe: 2.406 ± 0.242
4.287AspGly: 4.287 ± 0.462
0.7AspHis: 0.7 ± 0.155
4.331AspIle: 4.331 ± 0.564
3.193AspLys: 3.193 ± 0.311
5.424AspLeu: 5.424 ± 0.51
1.925AspMet: 1.925 ± 0.283
2.362AspAsn: 2.362 ± 0.321
2.712AspPro: 2.712 ± 0.336
2.012AspGln: 2.012 ± 0.295
2.537AspArg: 2.537 ± 0.287
3.718AspSer: 3.718 ± 0.355
3.674AspThr: 3.674 ± 0.366
3.587AspVal: 3.587 ± 0.451
0.831AspTrp: 0.831 ± 0.208
2.231AspTyr: 2.231 ± 0.356
0.0AspXaa: 0.0 ± 0.0
Glu
6.124GluAla: 6.124 ± 0.608
0.525GluCys: 0.525 ± 0.189
4.505GluAsp: 4.505 ± 0.534
5.205GluGlu: 5.205 ± 0.638
2.1GluPhe: 2.1 ± 0.305
3.631GluGly: 3.631 ± 0.33
1.137GluHis: 1.137 ± 0.187
2.843GluIle: 2.843 ± 0.397
3.631GluLys: 3.631 ± 0.4
6.474GluLeu: 6.474 ± 0.655
1.837GluMet: 1.837 ± 0.35
2.931GluAsn: 2.931 ± 0.476
2.362GluPro: 2.362 ± 0.31
3.324GluGln: 3.324 ± 0.525
2.8GluArg: 2.8 ± 0.462
3.412GluSer: 3.412 ± 0.441
3.412GluThr: 3.412 ± 0.428
4.68GluVal: 4.68 ± 0.583
0.787GluTrp: 0.787 ± 0.148
3.149GluTyr: 3.149 ± 0.499
0.0GluXaa: 0.0 ± 0.0
Phe
2.537PheAla: 2.537 ± 0.358
0.481PheCys: 0.481 ± 0.166
2.318PheAsp: 2.318 ± 0.243
1.662PheGlu: 1.662 ± 0.3
1.444PhePhe: 1.444 ± 0.278
2.712PheGly: 2.712 ± 0.353
0.569PheHis: 0.569 ± 0.128
2.362PheIle: 2.362 ± 0.381
1.925PheLys: 1.925 ± 0.318
2.625PheLeu: 2.625 ± 0.301
1.444PheMet: 1.444 ± 0.301
2.056PheAsn: 2.056 ± 0.327
0.919PhePro: 0.919 ± 0.194
1.575PheGln: 1.575 ± 0.26
1.793PheArg: 1.793 ± 0.27
1.75PheSer: 1.75 ± 0.296
3.106PheThr: 3.106 ± 0.503
2.1PheVal: 2.1 ± 0.323
0.525PheTrp: 0.525 ± 0.161
1.225PheTyr: 1.225 ± 0.246
0.0PheXaa: 0.0 ± 0.0
Gly
4.987GlyAla: 4.987 ± 0.735
0.744GlyCys: 0.744 ± 0.225
2.887GlyAsp: 2.887 ± 0.308
4.549GlyGlu: 4.549 ± 0.488
2.756GlyPhe: 2.756 ± 0.319
3.368GlyGly: 3.368 ± 0.333
0.962GlyHis: 0.962 ± 0.267
4.287GlyIle: 4.287 ± 0.453
5.38GlyLys: 5.38 ± 0.523
5.468GlyLeu: 5.468 ± 0.56
2.187GlyMet: 2.187 ± 0.341
4.593GlyAsn: 4.593 ± 0.515
1.225GlyPro: 1.225 ± 0.203
1.706GlyGln: 1.706 ± 0.266
3.149GlyArg: 3.149 ± 0.464
4.505GlySer: 4.505 ± 0.428
5.555GlyThr: 5.555 ± 0.503
4.068GlyVal: 4.068 ± 0.481
1.094GlyTrp: 1.094 ± 0.235
3.018GlyTyr: 3.018 ± 0.424
0.0GlyXaa: 0.0 ± 0.0
His
1.137HisAla: 1.137 ± 0.225
0.219HisCys: 0.219 ± 0.143
0.919HisAsp: 0.919 ± 0.211
0.962HisGlu: 0.962 ± 0.223
0.7HisPhe: 0.7 ± 0.213
1.094HisGly: 1.094 ± 0.276
0.481HisHis: 0.481 ± 0.154
1.225HisIle: 1.225 ± 0.24
1.356HisLys: 1.356 ± 0.252
1.837HisLeu: 1.837 ± 0.247
0.481HisMet: 0.481 ± 0.145
1.05HisAsn: 1.05 ± 0.173
0.962HisPro: 0.962 ± 0.265
0.7HisGln: 0.7 ± 0.14
0.962HisArg: 0.962 ± 0.217
0.962HisSer: 0.962 ± 0.157
0.744HisThr: 0.744 ± 0.202
1.05HisVal: 1.05 ± 0.276
0.394HisTrp: 0.394 ± 0.138
1.094HisTyr: 1.094 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
3.981IleAla: 3.981 ± 0.464
0.612IleCys: 0.612 ± 0.244
4.287IleAsp: 4.287 ± 0.496
3.893IleGlu: 3.893 ± 0.367
1.356IlePhe: 1.356 ± 0.243
4.374IleGly: 4.374 ± 0.503
1.487IleHis: 1.487 ± 0.333
3.324IleIle: 3.324 ± 0.476
4.418IleLys: 4.418 ± 0.753
3.368IleLeu: 3.368 ± 0.4
1.4IleMet: 1.4 ± 0.338
2.887IleAsn: 2.887 ± 0.306
2.756IlePro: 2.756 ± 0.353
2.931IleGln: 2.931 ± 0.388
2.756IleArg: 2.756 ± 0.367
3.237IleSer: 3.237 ± 0.407
4.199IleThr: 4.199 ± 0.418
3.499IleVal: 3.499 ± 0.431
0.7IleTrp: 0.7 ± 0.187
2.056IleTyr: 2.056 ± 0.263
0.0IleXaa: 0.0 ± 0.0
Lys
6.43LysAla: 6.43 ± 0.752
0.481LysCys: 0.481 ± 0.173
3.849LysAsp: 3.849 ± 0.436
4.287LysGlu: 4.287 ± 0.455
2.187LysPhe: 2.187 ± 0.313
3.237LysGly: 3.237 ± 0.334
1.4LysHis: 1.4 ± 0.286
2.8LysIle: 2.8 ± 0.387
3.456LysLys: 3.456 ± 0.632
6.343LysLeu: 6.343 ± 0.553
2.318LysMet: 2.318 ± 0.331
2.843LysAsn: 2.843 ± 0.384
3.368LysPro: 3.368 ± 0.458
2.581LysGln: 2.581 ± 0.327
2.931LysArg: 2.931 ± 0.322
3.762LysSer: 3.762 ± 0.339
4.112LysThr: 4.112 ± 0.591
5.162LysVal: 5.162 ± 0.468
0.787LysTrp: 0.787 ± 0.197
1.925LysTyr: 1.925 ± 0.295
0.0LysXaa: 0.0 ± 0.0
Leu
7.699LeuAla: 7.699 ± 0.596
0.919LeuCys: 0.919 ± 0.304
4.637LeuAsp: 4.637 ± 0.512
5.249LeuGlu: 5.249 ± 0.521
3.499LeuPhe: 3.499 ± 0.381
5.162LeuGly: 5.162 ± 0.528
1.094LeuHis: 1.094 ± 0.171
4.024LeuIle: 4.024 ± 0.407
5.205LeuLys: 5.205 ± 0.558
6.343LeuLeu: 6.343 ± 0.721
2.712LeuMet: 2.712 ± 0.381
4.724LeuAsn: 4.724 ± 0.516
4.024LeuPro: 4.024 ± 0.323
2.887LeuGln: 2.887 ± 0.377
3.412LeuArg: 3.412 ± 0.382
5.38LeuSer: 5.38 ± 0.471
5.643LeuThr: 5.643 ± 0.412
5.337LeuVal: 5.337 ± 0.404
1.137LeuTrp: 1.137 ± 0.185
2.581LeuTyr: 2.581 ± 0.359
0.0LeuXaa: 0.0 ± 0.0
Met
2.45MetAla: 2.45 ± 0.439
0.175MetCys: 0.175 ± 0.084
1.356MetAsp: 1.356 ± 0.207
1.968MetGlu: 1.968 ± 0.271
0.612MetPhe: 0.612 ± 0.178
2.187MetGly: 2.187 ± 0.26
0.831MetHis: 0.831 ± 0.213
1.837MetIle: 1.837 ± 0.278
2.056MetLys: 2.056 ± 0.267
2.143MetLeu: 2.143 ± 0.301
0.612MetMet: 0.612 ± 0.171
1.662MetAsn: 1.662 ± 0.266
1.312MetPro: 1.312 ± 0.239
1.662MetGln: 1.662 ± 0.281
1.356MetArg: 1.356 ± 0.182
2.493MetSer: 2.493 ± 0.345
1.925MetThr: 1.925 ± 0.321
2.362MetVal: 2.362 ± 0.309
0.219MetTrp: 0.219 ± 0.092
0.875MetTyr: 0.875 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
4.024AsnAla: 4.024 ± 0.444
0.394AsnCys: 0.394 ± 0.115
3.062AsnAsp: 3.062 ± 0.392
2.843AsnGlu: 2.843 ± 0.393
1.356AsnPhe: 1.356 ± 0.237
3.281AsnGly: 3.281 ± 0.443
1.312AsnHis: 1.312 ± 0.264
2.581AsnIle: 2.581 ± 0.286
3.718AsnLys: 3.718 ± 0.365
4.243AsnLeu: 4.243 ± 0.518
1.662AsnMet: 1.662 ± 0.296
2.887AsnAsn: 2.887 ± 0.416
3.718AsnPro: 3.718 ± 0.471
2.8AsnGln: 2.8 ± 0.422
3.062AsnArg: 3.062 ± 0.334
3.368AsnSer: 3.368 ± 0.393
3.674AsnThr: 3.674 ± 0.546
3.499AsnVal: 3.499 ± 0.43
0.7AsnTrp: 0.7 ± 0.176
2.187AsnTyr: 2.187 ± 0.349
0.0AsnXaa: 0.0 ± 0.0
Pro
3.587ProAla: 3.587 ± 0.349
0.219ProCys: 0.219 ± 0.113
2.843ProAsp: 2.843 ± 0.409
3.718ProGlu: 3.718 ± 0.327
1.75ProPhe: 1.75 ± 0.264
2.712ProGly: 2.712 ± 0.343
0.35ProHis: 0.35 ± 0.13
2.362ProIle: 2.362 ± 0.316
2.318ProLys: 2.318 ± 0.248
3.149ProLeu: 3.149 ± 0.284
1.312ProMet: 1.312 ± 0.23
2.056ProAsn: 2.056 ± 0.385
1.05ProPro: 1.05 ± 0.227
1.05ProGln: 1.05 ± 0.189
1.531ProArg: 1.531 ± 0.251
2.143ProSer: 2.143 ± 0.286
3.062ProThr: 3.062 ± 0.334
3.981ProVal: 3.981 ± 0.509
0.656ProTrp: 0.656 ± 0.228
1.356ProTyr: 1.356 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
4.068GlnAla: 4.068 ± 0.649
0.219GlnCys: 0.219 ± 0.101
2.012GlnAsp: 2.012 ± 0.36
2.668GlnGlu: 2.668 ± 0.376
1.225GlnPhe: 1.225 ± 0.2
2.887GlnGly: 2.887 ± 0.452
0.569GlnHis: 0.569 ± 0.168
2.187GlnIle: 2.187 ± 0.334
2.45GlnLys: 2.45 ± 0.324
3.674GlnLeu: 3.674 ± 0.431
1.312GlnMet: 1.312 ± 0.242
1.968GlnAsn: 1.968 ± 0.347
1.531GlnPro: 1.531 ± 0.381
2.318GlnGln: 2.318 ± 0.438
1.837GlnArg: 1.837 ± 0.309
2.8GlnSer: 2.8 ± 0.337
2.143GlnThr: 2.143 ± 0.379
2.581GlnVal: 2.581 ± 0.277
0.612GlnTrp: 0.612 ± 0.146
1.881GlnTyr: 1.881 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
3.456ArgAla: 3.456 ± 0.454
0.525ArgCys: 0.525 ± 0.192
2.756ArgAsp: 2.756 ± 0.304
2.625ArgGlu: 2.625 ± 0.296
1.444ArgPhe: 1.444 ± 0.259
2.974ArgGly: 2.974 ± 0.356
0.787ArgHis: 0.787 ± 0.209
2.712ArgIle: 2.712 ± 0.279
3.762ArgLys: 3.762 ± 0.409
4.331ArgLeu: 4.331 ± 0.487
1.837ArgMet: 1.837 ± 0.298
2.581ArgAsn: 2.581 ± 0.346
1.225ArgPro: 1.225 ± 0.224
1.662ArgGln: 1.662 ± 0.292
1.487ArgArg: 1.487 ± 0.315
2.362ArgSer: 2.362 ± 0.379
2.931ArgThr: 2.931 ± 0.345
2.931ArgVal: 2.931 ± 0.412
0.525ArgTrp: 0.525 ± 0.139
1.793ArgTyr: 1.793 ± 0.244
0.0ArgXaa: 0.0 ± 0.0
Ser
4.768SerAla: 4.768 ± 0.463
0.612SerCys: 0.612 ± 0.191
3.499SerAsp: 3.499 ± 0.382
3.587SerGlu: 3.587 ± 0.334
1.837SerPhe: 1.837 ± 0.312
4.112SerGly: 4.112 ± 0.476
0.787SerHis: 0.787 ± 0.182
3.631SerIle: 3.631 ± 0.414
3.762SerLys: 3.762 ± 0.469
4.637SerLeu: 4.637 ± 0.479
1.706SerMet: 1.706 ± 0.297
3.762SerAsn: 3.762 ± 0.436
2.537SerPro: 2.537 ± 0.352
2.318SerGln: 2.318 ± 0.385
2.931SerArg: 2.931 ± 0.476
3.762SerSer: 3.762 ± 0.518
4.812SerThr: 4.812 ± 0.731
3.981SerVal: 3.981 ± 0.488
0.525SerTrp: 0.525 ± 0.148
1.968SerTyr: 1.968 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
4.899ThrAla: 4.899 ± 0.674
0.394ThrCys: 0.394 ± 0.144
3.631ThrAsp: 3.631 ± 0.415
3.981ThrGlu: 3.981 ± 0.456
2.581ThrPhe: 2.581 ± 0.327
4.943ThrGly: 4.943 ± 0.524
1.356ThrHis: 1.356 ± 0.35
4.549ThrIle: 4.549 ± 0.534
4.331ThrLys: 4.331 ± 0.469
4.899ThrLeu: 4.899 ± 0.34
1.662ThrMet: 1.662 ± 0.332
3.718ThrAsn: 3.718 ± 0.491
3.018ThrPro: 3.018 ± 0.398
2.668ThrGln: 2.668 ± 0.34
2.625ThrArg: 2.625 ± 0.333
3.631ThrSer: 3.631 ± 0.63
3.412ThrThr: 3.412 ± 0.563
5.862ThrVal: 5.862 ± 0.682
1.05ThrTrp: 1.05 ± 0.258
2.843ThrTyr: 2.843 ± 0.431
0.0ThrXaa: 0.0 ± 0.0
Val
6.561ValAla: 6.561 ± 0.728
0.744ValCys: 0.744 ± 0.216
3.981ValAsp: 3.981 ± 0.374
4.768ValGlu: 4.768 ± 0.535
2.493ValPhe: 2.493 ± 0.417
5.293ValGly: 5.293 ± 0.742
1.837ValHis: 1.837 ± 0.236
3.849ValIle: 3.849 ± 0.564
3.981ValLys: 3.981 ± 0.354
4.505ValLeu: 4.505 ± 0.326
1.881ValMet: 1.881 ± 0.258
4.418ValAsn: 4.418 ± 0.44
2.887ValPro: 2.887 ± 0.261
2.8ValGln: 2.8 ± 0.326
3.368ValArg: 3.368 ± 0.401
3.587ValSer: 3.587 ± 0.441
4.855ValThr: 4.855 ± 0.543
5.774ValVal: 5.774 ± 0.649
0.656ValTrp: 0.656 ± 0.17
2.362ValTyr: 2.362 ± 0.312
0.0ValXaa: 0.0 ± 0.0
Trp
0.919TrpAla: 0.919 ± 0.216
0.175TrpCys: 0.175 ± 0.09
1.225TrpAsp: 1.225 ± 0.314
0.875TrpGlu: 0.875 ± 0.222
0.525TrpPhe: 0.525 ± 0.161
0.831TrpGly: 0.831 ± 0.191
0.35TrpHis: 0.35 ± 0.119
0.875TrpIle: 0.875 ± 0.245
0.7TrpLys: 0.7 ± 0.184
1.137TrpLeu: 1.137 ± 0.24
0.175TrpMet: 0.175 ± 0.093
0.787TrpAsn: 0.787 ± 0.183
0.262TrpPro: 0.262 ± 0.098
0.394TrpGln: 0.394 ± 0.119
0.612TrpArg: 0.612 ± 0.16
0.569TrpSer: 0.569 ± 0.191
0.569TrpThr: 0.569 ± 0.163
1.312TrpVal: 1.312 ± 0.241
0.087TrpTrp: 0.087 ± 0.063
0.481TrpTyr: 0.481 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.062TyrAla: 3.062 ± 0.366
0.394TyrCys: 0.394 ± 0.133
2.362TyrAsp: 2.362 ± 0.314
2.1TyrGlu: 2.1 ± 0.292
1.575TyrPhe: 1.575 ± 0.28
2.668TyrGly: 2.668 ± 0.437
0.962TyrHis: 0.962 ± 0.211
2.931TyrIle: 2.931 ± 0.525
2.45TyrLys: 2.45 ± 0.389
2.493TyrLeu: 2.493 ± 0.287
0.962TyrMet: 0.962 ± 0.239
2.231TyrAsn: 2.231 ± 0.33
1.925TyrPro: 1.925 ± 0.309
1.487TyrGln: 1.487 ± 0.271
1.444TyrArg: 1.444 ± 0.277
2.318TyrSer: 2.318 ± 0.289
2.1TyrThr: 2.1 ± 0.266
3.106TyrVal: 3.106 ± 0.515
0.437TyrTrp: 0.437 ± 0.125
1.356TyrTyr: 1.356 ± 0.257
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (22862 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski