Betapapillomavirus 5
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2AL34|A0A2D2AL34_9PAPI Regulatory protein E2 OS=Betapapillomavirus 5 OX=334209 GN=E2 PE=3 SV=1
MM1 pKa = 7.43 IGKK4 pKa = 8.97 EE5 pKa = 3.79 VTIPDD10 pKa = 4.17 IEE12 pKa = 5.64 LEE14 pKa = 4.19 LQDD17 pKa = 4.42 LVQPIDD23 pKa = 3.48 LHH25 pKa = 7.78 CDD27 pKa = 2.97 EE28 pKa = 5.09 VLPEE32 pKa = 3.94 EE33 pKa = 4.52 SEE35 pKa = 4.24 NLSEE39 pKa = 4.18 SSQAEE44 pKa = 4.21 VEE46 pKa = 4.15 PEE48 pKa = 3.96 RR49 pKa = 11.84 ILFKK53 pKa = 10.62 IVAPCGGCEE62 pKa = 3.8 TRR64 pKa = 11.84 LKK66 pKa = 10.39 IHH68 pKa = 6.63 IASTRR73 pKa = 11.84 FGIRR77 pKa = 11.84 CLEE80 pKa = 3.87 EE81 pKa = 4.56 LLLSEE86 pKa = 5.47 LCLLCPVCRR95 pKa = 11.84 NGRR98 pKa = 11.84 QQ99 pKa = 3.02
Molecular weight: 11.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.497
IPC2_protein 4.584
IPC_protein 4.444
Toseland 4.317
ProMoST 4.52
Dawson 4.368
Bjellqvist 4.52
Wikipedia 4.19
Rodwell 4.291
Grimsley 4.228
Solomon 4.355
Lehninger 4.304
Nozaki 4.469
DTASelect 4.546
Thurlkill 4.304
EMBOSS 4.215
Sillero 4.546
Patrickios 3.452
IPC_peptide 4.368
IPC2_peptide 4.546
IPC2.peptide.svr19 4.482
Protein with the highest isoelectric point:
>tr|A0A2D2AL37|A0A2D2AL37_9PAPI Minor capsid protein L2 OS=Betapapillomavirus 5 OX=334209 GN=L2 PE=3 SV=1
MM1 pKa = 7.45 EE2 pKa = 5.84 ALNQRR7 pKa = 11.84 FNVLQDD13 pKa = 3.4 QLMDD17 pKa = 3.31 IYY19 pKa = 11.21 EE20 pKa = 4.53 RR21 pKa = 11.84 GSDD24 pKa = 3.43 TLEE27 pKa = 3.75 EE28 pKa = 4.33 QIKK31 pKa = 9.81 HH32 pKa = 3.94 WTLLKK37 pKa = 10.1 QEE39 pKa = 4.31 QIILHH44 pKa = 4.89 YY45 pKa = 10.14 ARR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 GIMRR53 pKa = 11.84 LGYY56 pKa = 10.55 HH57 pKa = 6.55 PVPTLAVSEE66 pKa = 4.87 LKK68 pKa = 10.95 AKK70 pKa = 10.45 DD71 pKa = 3.73 AIAMVLHH78 pKa = 6.57 LEE80 pKa = 4.13 NLKK83 pKa = 10.32 QSPYY87 pKa = 10.46 KK88 pKa = 10.42 DD89 pKa = 3.96 EE90 pKa = 4.36 PWTLINTSLEE100 pKa = 4.4 TFRR103 pKa = 11.84 SPPANCFKK111 pKa = 10.83 KK112 pKa = 10.47 GPQNVEE118 pKa = 3.83 VLFDD122 pKa = 4.19 GDD124 pKa = 4.09 PEE126 pKa = 4.33 NVMLYY131 pKa = 7.93 TAWKK135 pKa = 8.95 FIYY138 pKa = 10.86 YY139 pKa = 10.09 EE140 pKa = 4.13 DD141 pKa = 5.56 TEE143 pKa = 4.56 GQWQKK148 pKa = 9.84 TEE150 pKa = 3.79 GHH152 pKa = 6.43 IDD154 pKa = 3.57 YY155 pKa = 10.98 AGLYY159 pKa = 8.59 YY160 pKa = 10.57 VEE162 pKa = 5.6 GGIKK166 pKa = 9.76 HH167 pKa = 6.0 YY168 pKa = 10.57 YY169 pKa = 10.02 VEE171 pKa = 4.78 FNVDD175 pKa = 2.93 AQRR178 pKa = 11.84 FGTRR182 pKa = 11.84 GEE184 pKa = 3.95 WEE186 pKa = 3.9 VRR188 pKa = 11.84 FNGEE192 pKa = 3.88 TLFAPVTSSSPSPLEE207 pKa = 4.35 EE208 pKa = 4.55 IGEE211 pKa = 4.4 RR212 pKa = 11.84 PKK214 pKa = 11.31 SPDD217 pKa = 3.27 VPEE220 pKa = 4.3 TTTDD224 pKa = 2.82 TGTLQRR230 pKa = 11.84 EE231 pKa = 4.27 TRR233 pKa = 11.84 HH234 pKa = 4.91 TEE236 pKa = 3.74 RR237 pKa = 11.84 AEE239 pKa = 3.89 STLPKK244 pKa = 9.62 GRR246 pKa = 11.84 RR247 pKa = 11.84 YY248 pKa = 8.84 EE249 pKa = 4.16 RR250 pKa = 11.84 KK251 pKa = 9.12 EE252 pKa = 3.77 SSPTSTSLRR261 pKa = 11.84 GRR263 pKa = 11.84 QKK265 pKa = 10.9 VSGHH269 pKa = 4.82 TSRR272 pKa = 11.84 KK273 pKa = 8.43 KK274 pKa = 8.75 EE275 pKa = 3.9 SRR277 pKa = 11.84 RR278 pKa = 11.84 SRR280 pKa = 11.84 STSSGRR286 pKa = 11.84 LGTDD290 pKa = 2.8 QAAARR295 pKa = 11.84 GRR297 pKa = 11.84 GQRR300 pKa = 11.84 QARR303 pKa = 11.84 PRR305 pKa = 11.84 TRR307 pKa = 11.84 SRR309 pKa = 11.84 SRR311 pKa = 11.84 RR312 pKa = 11.84 RR313 pKa = 11.84 SRR315 pKa = 11.84 SRR317 pKa = 11.84 SKK319 pKa = 11.05 GGGRR323 pKa = 11.84 TTAEE327 pKa = 4.1 SRR329 pKa = 11.84 RR330 pKa = 11.84 GRR332 pKa = 11.84 CGGRR336 pKa = 11.84 GYY338 pKa = 8.69 FTRR341 pKa = 11.84 SRR343 pKa = 11.84 SRR345 pKa = 11.84 SAEE348 pKa = 3.85 SRR350 pKa = 11.84 STDD353 pKa = 2.89 QCGILPSQVGTGVQSVGRR371 pKa = 11.84 KK372 pKa = 7.53 YY373 pKa = 10.16 SSRR376 pKa = 11.84 VEE378 pKa = 3.96 RR379 pKa = 11.84 LLAEE383 pKa = 4.47 AVDD386 pKa = 4.02 PPVILIRR393 pKa = 11.84 GPPNTLKK400 pKa = 10.6 CYY402 pKa = 10.23 RR403 pKa = 11.84 YY404 pKa = 9.67 RR405 pKa = 11.84 EE406 pKa = 4.05 KK407 pKa = 11.01 EE408 pKa = 4.02 RR409 pKa = 11.84 LKK411 pKa = 11.04 GFYY414 pKa = 10.43 DD415 pKa = 3.69 RR416 pKa = 11.84 FSTTWSWVGSEE427 pKa = 3.91 GNARR431 pKa = 11.84 LGRR434 pKa = 11.84 ARR436 pKa = 11.84 MLICFLNEE444 pKa = 3.55 EE445 pKa = 3.96 QRR447 pKa = 11.84 EE448 pKa = 4.05 RR449 pKa = 11.84 FIDD452 pKa = 4.05 KK453 pKa = 9.45 MRR455 pKa = 11.84 LPKK458 pKa = 10.68 GVDD461 pKa = 2.66 WSYY464 pKa = 12.12 GNFASII470 pKa = 4.49
Molecular weight: 53.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.341
IPC_protein 9.619
Toseland 9.984
ProMoST 9.765
Dawson 10.218
Bjellqvist 9.94
Wikipedia 10.423
Rodwell 10.438
Grimsley 10.306
Solomon 10.262
Lehninger 10.218
Nozaki 10.014
DTASelect 9.926
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.145
Patrickios 9.589
IPC_peptide 10.248
IPC2_peptide 8.741
IPC2.peptide.svr19 8.403
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2553
99
605
364.7
41.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.327 ± 0.204
2.35 ± 0.791
5.64 ± 0.483
7.521 ± 0.856
3.878 ± 0.424
7.09 ± 0.84
2.115 ± 0.23
4.779 ± 0.664
4.896 ± 0.76
8.931 ± 0.89
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.528 ± 0.457
4.152 ± 0.738
6.11 ± 1.166
4.505 ± 0.338
6.972 ± 1.24
7.286 ± 0.546
5.993 ± 0.977
6.11 ± 0.823
1.332 ± 0.366
3.486 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here