Phage NBSal001

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Tlsvirus; unclassified Tlsvirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8QY58|A0A6G8QY58_9CAUD Uncharacterized protein OS=Phage NBSal001 OX=2712975 PE=4 SV=1
MM1 pKa = 7.59FEE3 pKa = 4.5LKK5 pKa = 10.52SVTVEE10 pKa = 3.68ILNDD14 pKa = 3.56GSYY17 pKa = 11.43DD18 pKa = 3.68GMKK21 pKa = 10.4HH22 pKa = 4.36LTFPIRR28 pKa = 11.84IDD30 pKa = 3.15NCIDD34 pKa = 3.21YY35 pKa = 10.27GIEE38 pKa = 3.76VGMVDD43 pKa = 3.76VPLDD47 pKa = 3.57ALVAAGYY54 pKa = 10.3DD55 pKa = 3.69KK56 pKa = 11.4EE57 pKa = 4.6KK58 pKa = 10.6GQCADD63 pKa = 3.57IPDD66 pKa = 4.28SYY68 pKa = 11.6EE69 pKa = 4.43SDD71 pKa = 2.94THH73 pKa = 8.43LPFTYY78 pKa = 10.31GEE80 pKa = 4.21CRR82 pKa = 11.84IVDD85 pKa = 3.48

Molecular weight: 9.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8QY49|A0A6G8QY49_9CAUD Uncharacterized protein OS=Phage NBSal001 OX=2712975 PE=4 SV=1
MM1 pKa = 7.76TYY3 pKa = 10.79LKK5 pKa = 10.4NIKK8 pKa = 8.54TGRR11 pKa = 11.84IYY13 pKa = 10.67RR14 pKa = 11.84VNIRR18 pKa = 11.84HH19 pKa = 6.0KK20 pKa = 10.62NALIMSKK27 pKa = 10.07GGCWVRR33 pKa = 11.84SARR36 pKa = 11.84YY37 pKa = 9.21RR38 pKa = 11.84NAYY41 pKa = 7.77LTGYY45 pKa = 9.36PFIAVNSTNCC55 pKa = 3.54

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14462

37

1140

187.8

21.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.152 ± 0.42

1.39 ± 0.193

6.009 ± 0.222

7.088 ± 0.245

3.928 ± 0.144

7.129 ± 0.282

1.881 ± 0.244

6.776 ± 0.244

7.537 ± 0.378

6.707 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.188 ± 0.161

4.992 ± 0.226

3.291 ± 0.225

3.838 ± 0.336

4.84 ± 0.254

6.037 ± 0.262

5.442 ± 0.263

6.576 ± 0.245

1.466 ± 0.103

3.734 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski