CRESS virus sp. ct0Vt4
Average proteome isoelectric point is 7.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2WC63|A0A5Q2WC63_9VIRU Uncharacterized protein OS=CRESS virus sp. ct0Vt4 OX=2656673 PE=4 SV=1
MM1 pKa = 7.84 PCHH4 pKa = 6.52 PLLSRR9 pKa = 11.84 EE10 pKa = 4.19 DD11 pKa = 3.67 TGFSPYY17 pKa = 10.41 HH18 pKa = 5.32 NTSIHH23 pKa = 5.5 HH24 pKa = 6.43 TSPATAYY31 pKa = 10.28 GFEE34 pKa = 4.54 ANWNEE39 pKa = 4.04 EE40 pKa = 3.58 NRR42 pKa = 11.84 QNMSIGNWSSASSLRR57 pKa = 11.84 LDD59 pKa = 4.61 SEE61 pKa = 4.2 EE62 pKa = 5.05 LDD64 pKa = 4.21 PFSAMYY70 pKa = 9.28 TPNSVDD76 pKa = 3.25 QQLPTTTSGNRR87 pKa = 11.84 TLMLTEE93 pKa = 5.74 HH94 pKa = 7.17 DD95 pKa = 5.08 LNWDD99 pKa = 3.44 NDD101 pKa = 3.87 QLPEE105 pKa = 3.94 IQRR108 pKa = 11.84 LTGTLYY114 pKa = 11.08 GNQPSPEE121 pKa = 4.08 TLRR124 pKa = 11.84 PFPALYY130 pKa = 9.98 EE131 pKa = 4.12 YY132 pKa = 11.19 LHH134 pKa = 6.38 TPQSVVSRR142 pKa = 11.84 RR143 pKa = 11.84 TTLSPSPWNDD153 pKa = 2.84 TFQSMWDD160 pKa = 3.71 LLALASHH167 pKa = 6.62 EE168 pKa = 4.25 EE169 pKa = 3.86 HH170 pKa = 6.76 GQRR173 pKa = 11.84 QDD175 pKa = 3.23 LMPIPRR181 pKa = 11.84 TQIPSSGMGIRR192 pKa = 11.84 ARR194 pKa = 11.84 RR195 pKa = 11.84 TSSWTNSEE203 pKa = 3.92 ARR205 pKa = 11.84 STFPTYY211 pKa = 10.96 SDD213 pKa = 3.72 GQIDD217 pKa = 4.32 TPCPLRR223 pKa = 11.84 QRR225 pKa = 11.84 EE226 pKa = 3.73 VDD228 pKa = 3.75 AFSALQSFGSPAICTQCHH246 pKa = 7.19 GIPISTPKK254 pKa = 10.46 RR255 pKa = 11.84 KK256 pKa = 7.33 THH258 pKa = 5.78
Molecular weight: 29.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.445
IPC2_protein 5.385
IPC_protein 5.397
Toseland 5.779
ProMoST 5.601
Dawson 5.614
Bjellqvist 5.639
Wikipedia 5.601
Rodwell 5.588
Grimsley 5.944
Solomon 5.614
Lehninger 5.601
Nozaki 5.855
DTASelect 6.046
Thurlkill 6.008
EMBOSS 5.97
Sillero 5.944
Patrickios 3.872
IPC_peptide 5.626
IPC2_peptide 5.97
IPC2.peptide.svr19 5.897
Protein with the highest isoelectric point:
>tr|A0A5Q2WB21|A0A5Q2WB21_9VIRU Putative capsid protein OS=CRESS virus sp. ct0Vt4 OX=2656673 PE=4 SV=1
MM1 pKa = 7.16 TVNLSLLLRR10 pKa = 11.84 CISCKK15 pKa = 10.11 INLLRR20 pKa = 11.84 VLGQRR25 pKa = 11.84 LVLPTSSWKK34 pKa = 10.52 RR35 pKa = 11.84 LTCLGRR41 pKa = 11.84 RR42 pKa = 11.84 AEE44 pKa = 3.99 RR45 pKa = 11.84 LARR48 pKa = 11.84 KK49 pKa = 9.97 DD50 pKa = 3.16 IVLGLSTNKK59 pKa = 8.67 TRR61 pKa = 11.84 TTGLVFIFNFIYY73 pKa = 10.6
Molecular weight: 8.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.183
IPC2_protein 10.043
IPC_protein 11.359
Toseland 11.535
ProMoST 11.959
Dawson 11.55
Bjellqvist 11.506
Wikipedia 11.989
Rodwell 11.433
Grimsley 11.594
Solomon 11.989
Lehninger 11.901
Nozaki 11.535
DTASelect 11.506
Thurlkill 11.535
EMBOSS 12.018
Sillero 11.535
Patrickios 11.199
IPC_peptide 12.003
IPC2_peptide 10.979
IPC2.peptide.svr19 9.345
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1076
73
471
269.0
30.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.134 ± 0.593
1.115 ± 0.401
4.461 ± 0.647
4.647 ± 0.375
3.81 ± 0.443
6.506 ± 0.603
2.323 ± 0.494
4.647 ± 0.293
6.041 ± 1.816
7.807 ± 1.38
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.509 ± 0.177
5.019 ± 0.724
5.576 ± 1.077
3.81 ± 0.623
6.784 ± 0.609
8.178 ± 1.223
8.086 ± 0.813
6.227 ± 1.419
2.509 ± 0.485
3.81 ± 0.582
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here