Amino acid dipepetide frequency for Escherichia phage K30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.302AlaAla: 8.302 ± 1.063
0.791AlaCys: 0.791 ± 0.254
6.167AlaAsp: 6.167 ± 0.778
5.139AlaGlu: 5.139 ± 0.712
3.163AlaPhe: 3.163 ± 0.464
8.065AlaGly: 8.065 ± 1.216
1.344AlaHis: 1.344 ± 0.256
3.953AlaIle: 3.953 ± 0.524
6.404AlaLys: 6.404 ± 0.664
7.59AlaLeu: 7.59 ± 1.055
3.479AlaMet: 3.479 ± 0.579
4.349AlaAsn: 4.349 ± 0.521
2.846AlaPro: 2.846 ± 0.462
3.558AlaGln: 3.558 ± 0.555
4.586AlaArg: 4.586 ± 0.704
5.139AlaSer: 5.139 ± 0.523
4.19AlaThr: 4.19 ± 0.59
5.376AlaVal: 5.376 ± 0.601
1.265AlaTrp: 1.265 ± 0.343
2.925AlaTyr: 2.925 ± 0.509
0.0AlaXaa: 0.0 ± 0.0
Cys
0.395CysAla: 0.395 ± 0.183
0.158CysCys: 0.158 ± 0.123
0.395CysAsp: 0.395 ± 0.269
0.791CysGlu: 0.791 ± 0.275
0.395CysPhe: 0.395 ± 0.18
0.395CysGly: 0.395 ± 0.15
0.158CysHis: 0.158 ± 0.104
0.633CysIle: 0.633 ± 0.22
0.395CysLys: 0.395 ± 0.149
1.107CysLeu: 1.107 ± 0.348
0.158CysMet: 0.158 ± 0.158
0.237CysAsn: 0.237 ± 0.133
0.553CysPro: 0.553 ± 0.191
0.712CysGln: 0.712 ± 0.277
0.553CysArg: 0.553 ± 0.268
0.87CysSer: 0.87 ± 0.3
0.474CysThr: 0.474 ± 0.275
0.87CysVal: 0.87 ± 0.251
0.237CysTrp: 0.237 ± 0.136
0.316CysTyr: 0.316 ± 0.203
0.0CysXaa: 0.0 ± 0.0
Asp
5.534AspAla: 5.534 ± 0.599
0.395AspCys: 0.395 ± 0.214
4.981AspAsp: 4.981 ± 0.71
3.795AspGlu: 3.795 ± 0.547
2.925AspPhe: 2.925 ± 0.459
5.772AspGly: 5.772 ± 0.545
1.028AspHis: 1.028 ± 0.217
2.609AspIle: 2.609 ± 0.427
4.349AspLys: 4.349 ± 0.736
3.795AspLeu: 3.795 ± 0.525
2.056AspMet: 2.056 ± 0.385
2.53AspAsn: 2.53 ± 0.364
2.767AspPro: 2.767 ± 0.464
2.214AspGln: 2.214 ± 0.405
3.242AspArg: 3.242 ± 0.476
4.269AspSer: 4.269 ± 0.607
3.953AspThr: 3.953 ± 0.51
4.823AspVal: 4.823 ± 0.57
0.87AspTrp: 0.87 ± 0.278
2.53AspTyr: 2.53 ± 0.595
0.0AspXaa: 0.0 ± 0.0
Glu
7.827GluAla: 7.827 ± 1.034
0.633GluCys: 0.633 ± 0.244
4.111GluAsp: 4.111 ± 0.631
5.93GluGlu: 5.93 ± 0.979
2.767GluPhe: 2.767 ± 0.428
4.823GluGly: 4.823 ± 0.656
1.502GluHis: 1.502 ± 0.461
2.372GluIle: 2.372 ± 0.417
3.479GluLys: 3.479 ± 0.572
6.167GluLeu: 6.167 ± 1.004
1.581GluMet: 1.581 ± 0.538
2.056GluAsn: 2.056 ± 0.298
2.214GluPro: 2.214 ± 0.566
3.083GluGln: 3.083 ± 0.71
3.558GluArg: 3.558 ± 0.662
4.032GluSer: 4.032 ± 0.687
2.846GluThr: 2.846 ± 0.468
4.349GluVal: 4.349 ± 0.526
0.791GluTrp: 0.791 ± 0.285
3.004GluTyr: 3.004 ± 0.389
0.0GluXaa: 0.0 ± 0.0
Phe
2.688PheAla: 2.688 ± 0.458
0.395PheCys: 0.395 ± 0.204
2.53PheAsp: 2.53 ± 0.483
1.898PheGlu: 1.898 ± 0.334
0.949PhePhe: 0.949 ± 0.278
3.163PheGly: 3.163 ± 0.54
0.633PheHis: 0.633 ± 0.295
2.293PheIle: 2.293 ± 0.608
2.609PheLys: 2.609 ± 0.48
3.083PheLeu: 3.083 ± 0.582
1.028PheMet: 1.028 ± 0.245
2.056PheAsn: 2.056 ± 0.451
1.818PhePro: 1.818 ± 0.403
1.344PheGln: 1.344 ± 0.331
2.214PheArg: 2.214 ± 0.518
2.293PheSer: 2.293 ± 0.485
2.609PheThr: 2.609 ± 0.452
2.293PheVal: 2.293 ± 0.424
0.395PheTrp: 0.395 ± 0.144
1.028PheTyr: 1.028 ± 0.234
0.0PheXaa: 0.0 ± 0.0
Gly
6.799GlyAla: 6.799 ± 0.928
0.553GlyCys: 0.553 ± 0.255
5.218GlyAsp: 5.218 ± 0.617
5.06GlyGlu: 5.06 ± 0.653
2.925GlyPhe: 2.925 ± 0.401
6.325GlyGly: 6.325 ± 1.026
1.344GlyHis: 1.344 ± 0.296
4.902GlyIle: 4.902 ± 0.883
5.93GlyLys: 5.93 ± 0.792
5.772GlyLeu: 5.772 ± 0.7
2.135GlyMet: 2.135 ± 0.384
3.4GlyAsn: 3.4 ± 0.522
1.265GlyPro: 1.265 ± 0.389
2.925GlyGln: 2.925 ± 0.523
4.507GlyArg: 4.507 ± 0.372
6.088GlySer: 6.088 ± 0.802
4.032GlyThr: 4.032 ± 0.733
5.06GlyVal: 5.06 ± 0.73
1.66GlyTrp: 1.66 ± 0.478
3.083GlyTyr: 3.083 ± 0.47
0.0GlyXaa: 0.0 ± 0.0
His
0.949HisAla: 0.949 ± 0.283
0.712HisCys: 0.712 ± 0.219
1.186HisAsp: 1.186 ± 0.322
1.66HisGlu: 1.66 ± 0.408
0.949HisPhe: 0.949 ± 0.209
1.739HisGly: 1.739 ± 0.413
0.395HisHis: 0.395 ± 0.202
0.87HisIle: 0.87 ± 0.238
1.186HisLys: 1.186 ± 0.275
1.186HisLeu: 1.186 ± 0.332
0.712HisMet: 0.712 ± 0.184
0.316HisAsn: 0.316 ± 0.141
0.87HisPro: 0.87 ± 0.194
0.395HisGln: 0.395 ± 0.168
0.712HisArg: 0.712 ± 0.213
0.791HisSer: 0.791 ± 0.211
0.791HisThr: 0.791 ± 0.208
1.581HisVal: 1.581 ± 0.306
0.237HisTrp: 0.237 ± 0.123
0.87HisTyr: 0.87 ± 0.186
0.0HisXaa: 0.0 ± 0.0
Ile
4.269IleAla: 4.269 ± 0.468
0.553IleCys: 0.553 ± 0.176
3.4IleAsp: 3.4 ± 0.46
2.846IleGlu: 2.846 ± 0.488
0.791IlePhe: 0.791 ± 0.247
4.032IleGly: 4.032 ± 0.709
0.949IleHis: 0.949 ± 0.296
2.293IleIle: 2.293 ± 0.505
3.242IleLys: 3.242 ± 0.478
3.637IleLeu: 3.637 ± 0.496
1.107IleMet: 1.107 ± 0.328
2.372IleAsn: 2.372 ± 0.661
2.451IlePro: 2.451 ± 0.452
1.818IleGln: 1.818 ± 0.365
3.558IleArg: 3.558 ± 0.563
3.083IleSer: 3.083 ± 0.442
2.609IleThr: 2.609 ± 0.452
2.688IleVal: 2.688 ± 0.493
0.553IleTrp: 0.553 ± 0.192
1.739IleTyr: 1.739 ± 0.387
0.0IleXaa: 0.0 ± 0.0
Lys
7.985LysAla: 7.985 ± 1.077
0.474LysCys: 0.474 ± 0.216
3.479LysAsp: 3.479 ± 0.532
4.349LysGlu: 4.349 ± 0.725
2.53LysPhe: 2.53 ± 0.395
5.693LysGly: 5.693 ± 0.912
1.739LysHis: 1.739 ± 0.394
2.372LysIle: 2.372 ± 0.51
3.479LysLys: 3.479 ± 0.821
6.167LysLeu: 6.167 ± 0.752
1.66LysMet: 1.66 ± 0.36
2.609LysAsn: 2.609 ± 0.338
2.688LysPro: 2.688 ± 0.573
2.135LysGln: 2.135 ± 0.375
3.874LysArg: 3.874 ± 0.674
3.479LysSer: 3.479 ± 0.47
2.688LysThr: 2.688 ± 0.398
5.614LysVal: 5.614 ± 0.694
0.949LysTrp: 0.949 ± 0.331
2.056LysTyr: 2.056 ± 0.369
0.0LysXaa: 0.0 ± 0.0
Leu
7.432LeuAla: 7.432 ± 1.261
0.474LeuCys: 0.474 ± 0.189
4.823LeuAsp: 4.823 ± 0.565
6.72LeuGlu: 6.72 ± 0.946
2.846LeuPhe: 2.846 ± 0.433
5.455LeuGly: 5.455 ± 0.595
1.186LeuHis: 1.186 ± 0.313
3.795LeuIle: 3.795 ± 0.546
6.088LeuLys: 6.088 ± 0.737
6.246LeuLeu: 6.246 ± 0.831
2.135LeuMet: 2.135 ± 0.305
3.953LeuAsn: 3.953 ± 0.549
3.321LeuPro: 3.321 ± 0.518
3.242LeuGln: 3.242 ± 0.511
5.06LeuArg: 5.06 ± 0.541
4.428LeuSer: 4.428 ± 0.507
5.139LeuThr: 5.139 ± 0.694
5.06LeuVal: 5.06 ± 0.601
1.107LeuTrp: 1.107 ± 0.368
2.53LeuTyr: 2.53 ± 0.485
0.0LeuXaa: 0.0 ± 0.0
Met
3.083MetAla: 3.083 ± 0.486
0.158MetCys: 0.158 ± 0.102
1.898MetAsp: 1.898 ± 0.363
1.265MetGlu: 1.265 ± 0.305
0.791MetPhe: 0.791 ± 0.235
1.66MetGly: 1.66 ± 0.349
0.316MetHis: 0.316 ± 0.197
1.028MetIle: 1.028 ± 0.287
1.265MetLys: 1.265 ± 0.318
2.53MetLeu: 2.53 ± 0.53
0.791MetMet: 0.791 ± 0.297
1.028MetAsn: 1.028 ± 0.257
0.87MetPro: 0.87 ± 0.255
2.214MetGln: 2.214 ± 0.467
1.186MetArg: 1.186 ± 0.287
1.581MetSer: 1.581 ± 0.44
2.135MetThr: 2.135 ± 0.444
1.66MetVal: 1.66 ± 0.387
0.079MetTrp: 0.079 ± 0.079
0.712MetTyr: 0.712 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
4.349AsnAla: 4.349 ± 0.741
0.633AsnCys: 0.633 ± 0.316
2.53AsnAsp: 2.53 ± 0.431
2.609AsnGlu: 2.609 ± 0.549
1.423AsnPhe: 1.423 ± 0.323
4.349AsnGly: 4.349 ± 0.726
0.316AsnHis: 0.316 ± 0.156
2.925AsnIle: 2.925 ± 0.56
2.056AsnLys: 2.056 ± 0.327
2.925AsnLeu: 2.925 ± 0.442
0.87AsnMet: 0.87 ± 0.295
1.581AsnAsn: 1.581 ± 0.33
1.977AsnPro: 1.977 ± 0.309
1.502AsnGln: 1.502 ± 0.283
2.056AsnArg: 2.056 ± 0.581
3.004AsnSer: 3.004 ± 0.446
1.898AsnThr: 1.898 ± 0.298
3.083AsnVal: 3.083 ± 0.523
0.712AsnTrp: 0.712 ± 0.272
2.135AsnTyr: 2.135 ± 0.53
0.0AsnXaa: 0.0 ± 0.0
Pro
3.321ProAla: 3.321 ± 0.483
0.474ProCys: 0.474 ± 0.19
2.767ProAsp: 2.767 ± 0.41
4.111ProGlu: 4.111 ± 0.721
1.186ProPhe: 1.186 ± 0.278
2.372ProGly: 2.372 ± 0.447
0.553ProHis: 0.553 ± 0.187
0.949ProIle: 0.949 ± 0.32
2.53ProLys: 2.53 ± 0.467
2.846ProLeu: 2.846 ± 0.411
0.633ProMet: 0.633 ± 0.267
2.056ProAsn: 2.056 ± 0.444
1.265ProPro: 1.265 ± 0.412
1.502ProGln: 1.502 ± 0.254
1.66ProArg: 1.66 ± 0.433
2.135ProSer: 2.135 ± 0.332
1.818ProThr: 1.818 ± 0.417
3.004ProVal: 3.004 ± 0.437
0.712ProTrp: 0.712 ± 0.205
1.581ProTyr: 1.581 ± 0.404
0.0ProXaa: 0.0 ± 0.0
Gln
3.558GlnAla: 3.558 ± 0.508
0.158GlnCys: 0.158 ± 0.106
2.609GlnAsp: 2.609 ± 0.307
3.083GlnGlu: 3.083 ± 0.394
1.818GlnPhe: 1.818 ± 0.266
2.925GlnGly: 2.925 ± 0.509
0.474GlnHis: 0.474 ± 0.194
1.502GlnIle: 1.502 ± 0.389
3.083GlnLys: 3.083 ± 0.443
4.269GlnLeu: 4.269 ± 0.498
1.265GlnMet: 1.265 ± 0.447
1.186GlnAsn: 1.186 ± 0.267
1.502GlnPro: 1.502 ± 0.243
3.479GlnGln: 3.479 ± 0.607
2.451GlnArg: 2.451 ± 0.586
2.53GlnSer: 2.53 ± 0.404
1.818GlnThr: 1.818 ± 0.421
2.846GlnVal: 2.846 ± 0.414
0.791GlnTrp: 0.791 ± 0.238
1.66GlnTyr: 1.66 ± 0.489
0.0GlnXaa: 0.0 ± 0.0
Arg
4.586ArgAla: 4.586 ± 0.762
0.633ArgCys: 0.633 ± 0.276
3.795ArgAsp: 3.795 ± 0.554
3.479ArgGlu: 3.479 ± 0.591
2.135ArgPhe: 2.135 ± 0.43
3.716ArgGly: 3.716 ± 0.549
1.107ArgHis: 1.107 ± 0.291
3.4ArgIle: 3.4 ± 0.469
3.795ArgLys: 3.795 ± 0.687
4.665ArgLeu: 4.665 ± 0.56
1.265ArgMet: 1.265 ± 0.287
2.688ArgAsn: 2.688 ± 0.42
2.135ArgPro: 2.135 ± 0.367
2.846ArgGln: 2.846 ± 0.447
2.609ArgArg: 2.609 ± 0.327
3.242ArgSer: 3.242 ± 0.574
3.004ArgThr: 3.004 ± 0.503
3.558ArgVal: 3.558 ± 0.536
0.87ArgTrp: 0.87 ± 0.273
1.344ArgTyr: 1.344 ± 0.291
0.0ArgXaa: 0.0 ± 0.0
Ser
4.665SerAla: 4.665 ± 0.656
0.474SerCys: 0.474 ± 0.184
4.19SerAsp: 4.19 ± 0.56
3.558SerGlu: 3.558 ± 0.555
3.321SerPhe: 3.321 ± 0.467
4.507SerGly: 4.507 ± 0.709
1.818SerHis: 1.818 ± 0.354
2.925SerIle: 2.925 ± 0.489
4.586SerLys: 4.586 ± 0.584
4.665SerLeu: 4.665 ± 0.671
1.186SerMet: 1.186 ± 0.361
2.293SerAsn: 2.293 ± 0.538
1.739SerPro: 1.739 ± 0.318
2.925SerGln: 2.925 ± 0.444
3.4SerArg: 3.4 ± 0.556
3.637SerSer: 3.637 ± 0.688
4.349SerThr: 4.349 ± 0.776
4.032SerVal: 4.032 ± 0.491
0.791SerTrp: 0.791 ± 0.197
2.451SerTyr: 2.451 ± 0.389
0.0SerXaa: 0.0 ± 0.0
Thr
4.507ThrAla: 4.507 ± 0.717
0.87ThrCys: 0.87 ± 0.3
3.083ThrAsp: 3.083 ± 0.451
3.321ThrGlu: 3.321 ± 0.517
2.53ThrPhe: 2.53 ± 0.777
4.744ThrGly: 4.744 ± 0.634
0.791ThrHis: 0.791 ± 0.185
3.321ThrIle: 3.321 ± 0.526
4.586ThrLys: 4.586 ± 0.577
4.744ThrLeu: 4.744 ± 0.655
1.265ThrMet: 1.265 ± 0.283
1.739ThrAsn: 1.739 ± 0.468
2.925ThrPro: 2.925 ± 0.385
2.293ThrGln: 2.293 ± 0.41
2.53ThrArg: 2.53 ± 0.404
3.479ThrSer: 3.479 ± 0.608
3.558ThrThr: 3.558 ± 0.862
4.032ThrVal: 4.032 ± 0.754
0.553ThrTrp: 0.553 ± 0.189
1.423ThrTyr: 1.423 ± 0.324
0.0ThrXaa: 0.0 ± 0.0
Val
5.534ValAla: 5.534 ± 0.754
0.474ValCys: 0.474 ± 0.18
3.953ValAsp: 3.953 ± 0.401
3.874ValGlu: 3.874 ± 0.622
2.135ValPhe: 2.135 ± 0.577
5.376ValGly: 5.376 ± 0.622
1.502ValHis: 1.502 ± 0.4
3.716ValIle: 3.716 ± 0.527
4.032ValLys: 4.032 ± 0.524
6.009ValLeu: 6.009 ± 0.71
1.344ValMet: 1.344 ± 0.257
3.637ValAsn: 3.637 ± 0.807
2.609ValPro: 2.609 ± 0.461
2.372ValGln: 2.372 ± 0.475
4.032ValArg: 4.032 ± 0.54
4.665ValSer: 4.665 ± 0.969
5.139ValThr: 5.139 ± 0.643
4.744ValVal: 4.744 ± 0.652
1.028ValTrp: 1.028 ± 0.298
2.451ValTyr: 2.451 ± 0.528
0.0ValXaa: 0.0 ± 0.0
Trp
0.553TrpAla: 0.553 ± 0.204
0.474TrpCys: 0.474 ± 0.189
0.553TrpAsp: 0.553 ± 0.194
1.107TrpGlu: 1.107 ± 0.253
0.553TrpPhe: 0.553 ± 0.22
0.712TrpGly: 0.712 ± 0.249
0.316TrpHis: 0.316 ± 0.204
0.712TrpIle: 0.712 ± 0.291
1.344TrpLys: 1.344 ± 0.352
1.186TrpLeu: 1.186 ± 0.361
0.237TrpMet: 0.237 ± 0.127
0.633TrpAsn: 0.633 ± 0.232
0.474TrpPro: 0.474 ± 0.192
0.87TrpGln: 0.87 ± 0.284
0.949TrpArg: 0.949 ± 0.217
1.107TrpSer: 1.107 ± 0.394
0.712TrpThr: 0.712 ± 0.204
1.344TrpVal: 1.344 ± 0.338
0.316TrpTrp: 0.316 ± 0.135
0.316TrpTyr: 0.316 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.293TyrAla: 2.293 ± 0.445
0.316TyrCys: 0.316 ± 0.139
2.767TyrAsp: 2.767 ± 0.487
2.53TyrGlu: 2.53 ± 0.424
1.186TyrPhe: 1.186 ± 0.281
3.163TyrGly: 3.163 ± 0.44
0.633TyrHis: 0.633 ± 0.295
1.423TyrIle: 1.423 ± 0.49
1.581TyrLys: 1.581 ± 0.348
2.451TyrLeu: 2.451 ± 0.375
1.265TyrMet: 1.265 ± 0.327
2.214TyrAsn: 2.214 ± 0.482
1.265TyrPro: 1.265 ± 0.293
1.581TyrGln: 1.581 ± 0.378
2.135TyrArg: 2.135 ± 0.369
1.66TyrSer: 1.66 ± 0.387
2.53TyrThr: 2.53 ± 0.583
2.609TyrVal: 2.609 ± 0.529
0.553TyrTrp: 0.553 ± 0.207
0.949TyrTyr: 0.949 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (12649 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski