Intrasporangium oryzae NRRL B-24470

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Intrasporangium; Intrasporangium oryzae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4086 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9G8I1|W9G8I1_9MICO Tripartite tricarboxylate transporter TctA OS=Intrasporangium oryzae NRRL B-24470 OX=1386089 GN=N865_17710 PE=4 SV=1
MM1 pKa = 7.92SDD3 pKa = 3.51LDD5 pKa = 3.83YY6 pKa = 10.8PADD9 pKa = 3.51LRR11 pKa = 11.84YY12 pKa = 8.6TSDD15 pKa = 3.71HH16 pKa = 5.25EE17 pKa = 4.25WVRR20 pKa = 11.84VGEE23 pKa = 4.14GDD25 pKa = 3.69VVRR28 pKa = 11.84IGITAFAQDD37 pKa = 3.34ALGDD41 pKa = 3.82VVYY44 pKa = 11.12VSLPAVGDD52 pKa = 3.84TVAPGDD58 pKa = 3.39ACGEE62 pKa = 4.24VEE64 pKa = 4.37STKK67 pKa = 10.76SVSDD71 pKa = 3.56VYY73 pKa = 11.44APLAGEE79 pKa = 4.28VTATNAALDD88 pKa = 3.99ASPEE92 pKa = 4.4LINTDD97 pKa = 3.51PYY99 pKa = 10.99GEE101 pKa = 3.69GWMFEE106 pKa = 3.99VRR108 pKa = 11.84LADD111 pKa = 3.74TSALDD116 pKa = 3.67GLLDD120 pKa = 3.78AEE122 pKa = 4.72AYY124 pKa = 9.34KK125 pKa = 10.92AGLSS129 pKa = 3.64

Molecular weight: 13.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9G8B6|W9G8B6_9MICO Uncharacterized protein OS=Intrasporangium oryzae NRRL B-24470 OX=1386089 GN=N865_11865 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.61GRR40 pKa = 11.84AEE42 pKa = 3.84LSAA45 pKa = 4.93

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4086

0

4086

1346621

37

2235

329.6

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.381 ± 0.059

0.673 ± 0.01

6.204 ± 0.034

5.487 ± 0.042

2.768 ± 0.023

9.272 ± 0.031

2.248 ± 0.019

3.619 ± 0.027

1.867 ± 0.03

10.127 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.792 ± 0.015

1.721 ± 0.021

5.768 ± 0.026

2.608 ± 0.016

7.729 ± 0.044

5.508 ± 0.029

6.328 ± 0.037

9.418 ± 0.038

1.59 ± 0.018

1.893 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski