Torque teno mini virus 9
Average proteome isoelectric point is 7.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9JG48|Q9JG48_9VIRU Uncharacterized protein OS=Torque teno mini virus 9 OX=687377 PE=4 SV=1
MM1 pKa = 7.31 SRR3 pKa = 11.84 YY4 pKa = 9.61 IPTKK8 pKa = 9.06 STLRR12 pKa = 11.84 QKK14 pKa = 10.73 KK15 pKa = 7.99 LQWMNLIVHH24 pKa = 6.23 GHH26 pKa = 7.38 DD27 pKa = 4.74 IFCDD31 pKa = 3.33 CCKK34 pKa = 10.24 PLEE37 pKa = 4.26 CTIGTIINQEE47 pKa = 4.07 PNLKK51 pKa = 10.61 FNTEE55 pKa = 3.94 EE56 pKa = 4.04 KK57 pKa = 10.9 NLLKK61 pKa = 10.67 KK62 pKa = 10.66 CLSTKK67 pKa = 10.83 DD68 pKa = 3.22 GDD70 pKa = 4.27 AGAGAADD77 pKa = 3.69 PDD79 pKa = 4.35 GFGEE83 pKa = 4.57 GDD85 pKa = 3.69 LDD87 pKa = 4.63 ALFTEE92 pKa = 5.51 DD93 pKa = 4.86 FGEE96 pKa = 4.41 EE97 pKa = 3.91 NTGG100 pKa = 3.27
Molecular weight: 11.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.732
IPC2_protein 4.787
IPC_protein 4.66
Toseland 4.507
ProMoST 4.774
Dawson 4.622
Bjellqvist 4.762
Wikipedia 4.507
Rodwell 4.507
Grimsley 4.418
Solomon 4.609
Lehninger 4.571
Nozaki 4.736
DTASelect 4.902
Thurlkill 4.52
EMBOSS 4.52
Sillero 4.787
Patrickios 3.897
IPC_peptide 4.622
IPC2_peptide 4.774
IPC2.peptide.svr19 4.767
Protein with the highest isoelectric point:
>tr|Q9JG47|Q9JG47_9VIRU Capsid protein OS=Torque teno mini virus 9 OX=687377 PE=3 SV=1
MM1 pKa = 7.54 NNQPIPSPITSYY13 pKa = 10.73 KK14 pKa = 9.03 QLRR17 pKa = 11.84 CRR19 pKa = 11.84 VQQHH23 pKa = 6.26 HH24 pKa = 7.37 LNTSSGTLTNAEE36 pKa = 3.91 EE37 pKa = 4.21 SLQKK41 pKa = 10.35 EE42 pKa = 4.32 LQKK45 pKa = 11.09 EE46 pKa = 3.97 LLKK49 pKa = 10.23 TSKK52 pKa = 9.96 LKK54 pKa = 10.96 QMFCQLQSQQPGVQQHH70 pKa = 4.87 TEE72 pKa = 3.19 KK73 pKa = 10.82 HH74 pKa = 4.62 KK75 pKa = 7.02 THH77 pKa = 6.6 RR78 pKa = 11.84 RR79 pKa = 11.84 HH80 pKa = 6.73 RR81 pKa = 11.84 PRR83 pKa = 11.84 KK84 pKa = 7.13 KK85 pKa = 7.9 TKK87 pKa = 8.58 HH88 pKa = 4.09 SRR90 pKa = 11.84 RR91 pKa = 11.84 RR92 pKa = 11.84 RR93 pKa = 11.84 NYY95 pKa = 9.65 SSSEE99 pKa = 3.73 EE100 pKa = 4.29 SKK102 pKa = 11.13 NSSTNSSDD110 pKa = 4.81 DD111 pKa = 3.79 SYY113 pKa = 12.21 SDD115 pKa = 3.25
Molecular weight: 13.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.199
IPC2_protein 9.472
IPC_protein 9.545
Toseland 10.467
ProMoST 9.984
Dawson 10.57
Bjellqvist 10.175
Wikipedia 10.687
Rodwell 11.096
Grimsley 10.599
Solomon 10.599
Lehninger 10.584
Nozaki 10.438
DTASelect 10.16
Thurlkill 10.452
EMBOSS 10.833
Sillero 10.482
Patrickios 10.847
IPC_peptide 10.613
IPC2_peptide 8.814
IPC2.peptide.svr19 8.507
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
898
100
683
299.3
34.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.563 ± 0.821
2.004 ± 0.936
4.232 ± 1.445
5.011 ± 1.114
3.675 ± 0.844
5.011 ± 1.595
2.561 ± 1.062
4.454 ± 0.835
7.795 ± 1.021
9.688 ± 0.56
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.45 ± 0.302
5.902 ± 0.074
6.125 ± 1.01
5.791 ± 1.67
6.57 ± 1.398
6.347 ± 2.765
8.797 ± 0.457
2.895 ± 0.802
2.339 ± 0.963
4.788 ± 1.575
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here