archaeon D22

Taxonomy: cellular organisms; Archaea; unclassified Archaea

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 474 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202DFH1|A0A202DFH1_9ARCH Uncharacterized protein OS=archaeon D22 OX=1932700 GN=BVX95_01350 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.34KK3 pKa = 9.74IGIMMLIFATLMIAGCSSPEE23 pKa = 3.68ASVCGDD29 pKa = 3.9GVCSFLEE36 pKa = 4.04KK37 pKa = 10.97SKK39 pKa = 11.18DD40 pKa = 3.65NCPMDD45 pKa = 4.44CKK47 pKa = 10.9ARR49 pKa = 11.84APDD52 pKa = 3.63LSLDD56 pKa = 3.53VEE58 pKa = 4.56FDD60 pKa = 3.17GGTVDD65 pKa = 4.7AYY67 pKa = 11.51SDD69 pKa = 3.98LVLDD73 pKa = 3.33YY74 pKa = 10.18TIRR77 pKa = 11.84NRR79 pKa = 11.84GDD81 pKa = 3.27LATKK85 pKa = 9.72FVVEE89 pKa = 4.4FDD91 pKa = 4.16VEE93 pKa = 4.18RR94 pKa = 11.84LDD96 pKa = 3.98EE97 pKa = 4.39NEE99 pKa = 3.98EE100 pKa = 3.85YY101 pKa = 10.65SYY103 pKa = 11.82GRR105 pKa = 11.84GYY107 pKa = 10.71SRR109 pKa = 11.84QEE111 pKa = 3.41PVMLGEE117 pKa = 4.91GEE119 pKa = 4.21NFNDD123 pKa = 5.21KK124 pKa = 11.21ANFWIRR130 pKa = 11.84EE131 pKa = 3.95EE132 pKa = 4.16GNYY135 pKa = 9.62RR136 pKa = 11.84ISMKK140 pKa = 9.83TYY142 pKa = 9.06PVNEE146 pKa = 3.49RR147 pKa = 11.84DD148 pKa = 4.8SFNEE152 pKa = 3.83DD153 pKa = 2.61NSYY156 pKa = 10.73EE157 pKa = 4.46AIVSAAKK164 pKa = 9.91PSYY167 pKa = 9.37PEE169 pKa = 3.94YY170 pKa = 10.31IIEE173 pKa = 4.11EE174 pKa = 4.36NISAFEE180 pKa = 3.84YY181 pKa = 10.51QYY183 pKa = 10.34TRR185 pKa = 11.84KK186 pKa = 8.68WDD188 pKa = 3.39NGIDD192 pKa = 3.71FDD194 pKa = 4.96EE195 pKa = 6.11IEE197 pKa = 5.18AEE199 pKa = 4.15STYY202 pKa = 10.5NAQYY206 pKa = 10.68EE207 pKa = 4.44YY208 pKa = 11.25VDD210 pKa = 3.99EE211 pKa = 4.74YY212 pKa = 11.36EE213 pKa = 4.57NSAQIDD219 pKa = 4.35LEE221 pKa = 4.41VTTYY225 pKa = 10.71GQDD228 pKa = 2.83ITVEE232 pKa = 3.79ARR234 pKa = 11.84DD235 pKa = 3.76FFMSYY240 pKa = 10.78SSFEE244 pKa = 4.0AEE246 pKa = 4.35NYY248 pKa = 9.92KK249 pKa = 10.9GDD251 pKa = 3.76IIFIDD256 pKa = 4.26GEE258 pKa = 4.74DD259 pKa = 3.69YY260 pKa = 11.63GDD262 pKa = 3.32MQFFEE267 pKa = 5.13VVWPSNDD274 pKa = 2.84KK275 pKa = 10.63LVRR278 pKa = 11.84LFIHH282 pKa = 6.58FNEE285 pKa = 4.55KK286 pKa = 10.47EE287 pKa = 3.77MDD289 pKa = 3.28GDD291 pKa = 4.09YY292 pKa = 10.94IEE294 pKa = 5.39EE295 pKa = 4.12FVDD298 pKa = 3.98KK299 pKa = 10.85YY300 pKa = 10.76LAKK303 pKa = 10.2FDD305 pKa = 4.22SSVEE309 pKa = 3.89YY310 pKa = 11.44NMLCGDD316 pKa = 4.84NKK318 pKa = 10.49CQYY321 pKa = 11.51NNIFLQQSEE330 pKa = 5.14TYY332 pKa = 9.0STEE335 pKa = 4.07FNDD338 pKa = 3.84NNVHH342 pKa = 6.57EE343 pKa = 4.6ISVLNISDD351 pKa = 3.46TGAQITIDD359 pKa = 3.73DD360 pKa = 3.69DD361 pKa = 4.47TYY363 pKa = 11.7FEE365 pKa = 4.15FSEE368 pKa = 3.97IGEE371 pKa = 4.29VVDD374 pKa = 4.78YY375 pKa = 10.89NGQSIEE381 pKa = 4.18LRR383 pKa = 11.84NLAPTEE389 pKa = 3.77IEE391 pKa = 4.86FIDD394 pKa = 4.03FTSSNVEE401 pKa = 3.74IVVATINEE409 pKa = 4.02EE410 pKa = 4.29GNLKK414 pKa = 10.32NYY416 pKa = 10.41EE417 pKa = 4.21EE418 pKa = 5.35IEE420 pKa = 4.23VQLGKK425 pKa = 10.44EE426 pKa = 3.81YY427 pKa = 10.95SVNFDD432 pKa = 3.4EE433 pKa = 5.49GNYY436 pKa = 9.95SIKK439 pKa = 10.51LIKK442 pKa = 10.38FKK444 pKa = 11.07EE445 pKa = 4.14DD446 pKa = 3.17VAVIEE451 pKa = 4.37VNDD454 pKa = 4.08DD455 pKa = 3.46KK456 pKa = 11.65EE457 pKa = 5.15SIILHH462 pKa = 5.39EE463 pKa = 4.28EE464 pKa = 4.05EE465 pKa = 5.26IIGGLYY471 pKa = 10.31VRR473 pKa = 11.84VDD475 pKa = 4.67DD476 pKa = 5.32IDD478 pKa = 4.16IDD480 pKa = 4.02FDD482 pKa = 3.99TEE484 pKa = 4.06TRR486 pKa = 11.84PEE488 pKa = 3.83AVILNIGEE496 pKa = 4.3NYY498 pKa = 8.64QACPSDD504 pKa = 4.26CAPMVAQEE512 pKa = 4.3EE513 pKa = 4.41PMPVSSSGGSAGEE526 pKa = 4.22SGPVMGSEE534 pKa = 4.43VEE536 pKa = 3.98LSEE539 pKa = 4.49

Molecular weight: 61.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202DG21|A0A202DG21_9ARCH Uncharacterized protein OS=archaeon D22 OX=1932700 GN=BVX95_00645 PE=4 SV=1
MM1 pKa = 7.46TFFTSFILGLLTPLTAVCVLPLVPGFLVYY30 pKa = 10.8LSNQIGTQTSKK41 pKa = 10.97LRR43 pKa = 11.84TLMLGVIMTLGVILFMGLIGVVFSLLLQISLTNVIGIISPIAFGTLAIFSILLIFNVDD101 pKa = 2.75IGKK104 pKa = 9.32FLPKK108 pKa = 10.34FKK110 pKa = 11.06SPVAKK115 pKa = 10.5SPILSSFLFGFFFGAIVLPCNPLFIAAMFSRR146 pKa = 11.84ALTFTDD152 pKa = 3.17LTMNIINFIFFGFGMGFPLIFLSAVSAATGTKK184 pKa = 9.84LVSFLSKK191 pKa = 10.59NKK193 pKa = 10.22RR194 pKa = 11.84ILNLISGVIMLGISIYY210 pKa = 11.42YY211 pKa = 7.82MFFVFRR217 pKa = 11.84ILRR220 pKa = 3.85

Molecular weight: 24.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

474

0

474

147978

39

4423

312.2

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.522 ± 0.124

1.008 ± 0.052

6.281 ± 0.152

7.623 ± 0.172

4.885 ± 0.115

6.252 ± 0.127

1.693 ± 0.05

8.492 ± 0.135

8.504 ± 0.249

8.907 ± 0.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.075

5.832 ± 0.21

3.108 ± 0.064

2.628 ± 0.066

3.442 ± 0.113

7.073 ± 0.177

5.24 ± 0.178

6.546 ± 0.098

0.762 ± 0.038

3.978 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski