Lindernia anagallis yellow vein virus satellite DNA beta
Average proteome isoelectric point is 5.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A5H1F9|A5H1F9_9VIRU C1 protein OS=Lindernia anagallis yellow vein virus satellite DNA beta OX=447605 GN=C1 PE=4 SV=1
MM1 pKa = 7.6 TIRR4 pKa = 11.84 YY5 pKa = 6.39 QTSKK9 pKa = 10.71 NIHH12 pKa = 5.05 FTIDD16 pKa = 3.02 VHH18 pKa = 6.46 LKK20 pKa = 9.96 NRR22 pKa = 11.84 VTVTIRR28 pKa = 11.84 LIYY31 pKa = 9.16 TRR33 pKa = 11.84 SPVMTAITYY42 pKa = 7.39 NIPYY46 pKa = 8.56 TAEE49 pKa = 3.9 EE50 pKa = 4.8 NIPPPIITSEE60 pKa = 3.85 RR61 pKa = 11.84 DD62 pKa = 3.05 GAEE65 pKa = 3.89 DD66 pKa = 4.36 HH67 pKa = 6.59 IHH69 pKa = 6.73 HH70 pKa = 7.16 ILYY73 pKa = 10.52 AIYY76 pKa = 10.53 DD77 pKa = 3.83 DD78 pKa = 5.53 VDD80 pKa = 3.43 IKK82 pKa = 11.1 DD83 pKa = 4.78 FKK85 pKa = 11.16 PEE87 pKa = 3.79 MMLDD91 pKa = 3.4 AVDD94 pKa = 3.48 IMMMDD99 pKa = 3.63 RR100 pKa = 11.84 FDD102 pKa = 5.54 HH103 pKa = 6.89 IGIDD107 pKa = 3.62 TDD109 pKa = 4.27 GSCRR113 pKa = 11.84 IRR115 pKa = 11.84 TIHH118 pKa = 6.32 NVV120 pKa = 2.87
Molecular weight: 13.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.377
IPC2_protein 5.283
IPC_protein 5.27
Toseland 5.664
ProMoST 5.588
Dawson 5.486
Bjellqvist 5.525
Wikipedia 5.486
Rodwell 5.448
Grimsley 5.855
Solomon 5.486
Lehninger 5.474
Nozaki 5.728
DTASelect 5.931
Thurlkill 5.855
EMBOSS 5.83
Sillero 5.804
Patrickios 3.859
IPC_peptide 5.499
IPC2_peptide 5.83
IPC2.peptide.svr19 5.77
Protein with the highest isoelectric point:
>tr|A5H1F9|A5H1F9_9VIRU C1 protein OS=Lindernia anagallis yellow vein virus satellite DNA beta OX=447605 GN=C1 PE=4 SV=1
MM1 pKa = 7.6 TIRR4 pKa = 11.84 YY5 pKa = 6.39 QTSKK9 pKa = 10.71 NIHH12 pKa = 5.05 FTIDD16 pKa = 3.02 VHH18 pKa = 6.46 LKK20 pKa = 9.96 NRR22 pKa = 11.84 VTVTIRR28 pKa = 11.84 LIYY31 pKa = 9.16 TRR33 pKa = 11.84 SPVMTAITYY42 pKa = 7.39 NIPYY46 pKa = 8.56 TAEE49 pKa = 3.9 EE50 pKa = 4.8 NIPPPIITSEE60 pKa = 3.85 RR61 pKa = 11.84 DD62 pKa = 3.05 GAEE65 pKa = 3.89 DD66 pKa = 4.36 HH67 pKa = 6.59 IHH69 pKa = 6.73 HH70 pKa = 7.16 ILYY73 pKa = 10.52 AIYY76 pKa = 10.53 DD77 pKa = 3.83 DD78 pKa = 5.53 VDD80 pKa = 3.43 IKK82 pKa = 11.1 DD83 pKa = 4.78 FKK85 pKa = 11.16 PEE87 pKa = 3.79 MMLDD91 pKa = 3.4 AVDD94 pKa = 3.48 IMMMDD99 pKa = 3.63 RR100 pKa = 11.84 FDD102 pKa = 5.54 HH103 pKa = 6.89 IGIDD107 pKa = 3.62 TDD109 pKa = 4.27 GSCRR113 pKa = 11.84 IRR115 pKa = 11.84 TIHH118 pKa = 6.32 NVV120 pKa = 2.87
Molecular weight: 13.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.377
IPC2_protein 5.283
IPC_protein 5.27
Toseland 5.664
ProMoST 5.588
Dawson 5.486
Bjellqvist 5.525
Wikipedia 5.486
Rodwell 5.448
Grimsley 5.855
Solomon 5.486
Lehninger 5.474
Nozaki 5.728
DTASelect 5.931
Thurlkill 5.855
EMBOSS 5.83
Sillero 5.804
Patrickios 3.859
IPC_peptide 5.499
IPC2_peptide 5.83
IPC2.peptide.svr19 5.77
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
120
120
120
120.0
13.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.167 ± 0.0
0.833 ± 0.0
10.833 ± 0.0
4.167 ± 0.0
2.5 ± 0.0
2.5 ± 0.0
5.833 ± 0.0
15.833 ± 0.0
3.333 ± 0.0
3.333 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.833 ± 0.0
4.167 ± 0.0
5.0 ± 0.0
0.833 ± 0.0
6.667 ± 0.0
3.333 ± 0.0
10.0 ± 0.0
5.833 ± 0.0
0.0 ± 0.0
5.0 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here