Beihai permutotetra-like virus 2
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHU9|A0A1L3KHU9_9VIRU RdRp OS=Beihai permutotetra-like virus 2 OX=1922513 PE=4 SV=1
MM1 pKa = 7.87 DD2 pKa = 5.44 ASNPVSSGDD11 pKa = 3.35 KK12 pKa = 9.72 VTFADD17 pKa = 4.27 LKK19 pKa = 10.86 EE20 pKa = 4.61 DD21 pKa = 3.5 SLLLKK26 pKa = 10.12 RR27 pKa = 11.84 QNFEE31 pKa = 3.98 EE32 pKa = 4.18 LKK34 pKa = 10.65 KK35 pKa = 11.14 LIGTSPRR42 pKa = 11.84 TPAPYY47 pKa = 10.01 VGRR50 pKa = 11.84 NEE52 pKa = 3.67 YY53 pKa = 10.68 DD54 pKa = 3.3 YY55 pKa = 11.83 DD56 pKa = 4.17 EE57 pKa = 6.01 DD58 pKa = 4.58 VVKK61 pKa = 10.99 DD62 pKa = 4.35 LIKK65 pKa = 10.56 PIAVAKK71 pKa = 9.9 GNPEE75 pKa = 3.84 VGFLTEE81 pKa = 3.7 AAKK84 pKa = 10.77 VRR86 pKa = 11.84 VCSFHH91 pKa = 6.77 TMGEE95 pKa = 4.1 NQEE98 pKa = 4.37 DD99 pKa = 4.15 NNVSMKK105 pKa = 8.78 QTYY108 pKa = 10.0 KK109 pKa = 9.54 IAKK112 pKa = 8.67 RR113 pKa = 11.84 DD114 pKa = 3.5 RR115 pKa = 11.84 KK116 pKa = 10.23 LVEE119 pKa = 3.88 VCLKK123 pKa = 10.82 SGAQSDD129 pKa = 4.1 DD130 pKa = 3.1 IKK132 pKa = 11.16 RR133 pKa = 11.84 LIYY136 pKa = 8.2 TTGNKK141 pKa = 9.8 EE142 pKa = 4.09 GFSKK146 pKa = 10.68 RR147 pKa = 11.84 LRR149 pKa = 11.84 LWATKK154 pKa = 9.84 KK155 pKa = 10.6 VPSPKK160 pKa = 9.64 TAMEE164 pKa = 4.21 KK165 pKa = 10.71 YY166 pKa = 9.52 NIKK169 pKa = 10.67 FEE171 pKa = 4.41 DD172 pKa = 3.35 VRR174 pKa = 11.84 AALPVGKK181 pKa = 10.14 GLPNWNGNIEE191 pKa = 4.08 EE192 pKa = 4.81 FISGVRR198 pKa = 11.84 VNKK201 pKa = 10.45 SSGAGPPFYY210 pKa = 9.65 RR211 pKa = 11.84 TKK213 pKa = 10.51 RR214 pKa = 11.84 QCLDD218 pKa = 3.35 DD219 pKa = 4.76 CFDD222 pKa = 6.11 AIGKK226 pKa = 8.84 LIKK229 pKa = 9.9 EE230 pKa = 4.48 ANQDD234 pKa = 3.59 HH235 pKa = 7.2 LDD237 pKa = 3.31 KK238 pKa = 10.78 FLRR241 pKa = 11.84 EE242 pKa = 3.95 NEE244 pKa = 4.1 EE245 pKa = 4.72 FLVCQCKK252 pKa = 10.52 NKK254 pKa = 8.99 TDD256 pKa = 3.94 RR257 pKa = 11.84 YY258 pKa = 9.89 PPDD261 pKa = 3.56 KK262 pKa = 10.52 LHH264 pKa = 6.79 EE265 pKa = 4.07 KK266 pKa = 8.13 TRR268 pKa = 11.84 PYY270 pKa = 10.79 FSFSFPTQFLCSALNQPFTDD290 pKa = 3.38 NMLLFHH296 pKa = 7.15 EE297 pKa = 5.33 SGANACGFSYY307 pKa = 11.4 ADD309 pKa = 3.91 GGSQRR314 pKa = 11.84 LFDD317 pKa = 3.79 WMCDD321 pKa = 3.46 TEE323 pKa = 4.15 EE324 pKa = 6.03 GEE326 pKa = 5.22 VKK328 pKa = 10.45 FCAYY332 pKa = 10.38 GDD334 pKa = 3.7 DD335 pKa = 3.31 VRR337 pKa = 11.84 MVKK340 pKa = 10.38 RR341 pKa = 11.84 EE342 pKa = 3.94 DD343 pKa = 3.44 GVLYY347 pKa = 10.61 SCSPDD352 pKa = 3.69 FQCMDD357 pKa = 3.31 GSIHH361 pKa = 5.45 KK362 pKa = 8.91 TEE364 pKa = 3.69 AAEE367 pKa = 3.96 YY368 pKa = 9.43 VDD370 pKa = 5.28 YY371 pKa = 11.24 VLHH374 pKa = 6.52 CYY376 pKa = 8.7 EE377 pKa = 4.87 RR378 pKa = 11.84 EE379 pKa = 4.09 HH380 pKa = 6.96 GPSNFWKK387 pKa = 10.57 FVGEE391 pKa = 4.09 VWKK394 pKa = 10.36 KK395 pKa = 9.59 QLVGAKK401 pKa = 9.45 FFVNGSQPYY410 pKa = 9.38 RR411 pKa = 11.84 SEE413 pKa = 3.98 TGLLTGCVGTTGVDD427 pKa = 3.25 TFKK430 pKa = 11.19 SVTAFVTLIEE440 pKa = 4.09 AHH442 pKa = 5.91 KK443 pKa = 10.16 HH444 pKa = 4.64 YY445 pKa = 10.69 GVDD448 pKa = 2.9 IMNVEE453 pKa = 4.08 EE454 pKa = 4.57 VKK456 pKa = 11.21 NFMRR460 pKa = 11.84 EE461 pKa = 3.76 NFGLVLKK468 pKa = 10.71 GDD470 pKa = 4.2 TYY472 pKa = 11.39 QWDD475 pKa = 3.91 EE476 pKa = 4.04 VQEE479 pKa = 3.97 EE480 pKa = 4.69 LEE482 pKa = 4.5 EE483 pKa = 4.82 GEE485 pKa = 4.3 QPNAQEE491 pKa = 3.99 FLGVQLKK498 pKa = 10.57 AVTGKK503 pKa = 9.82 NRR505 pKa = 11.84 LEE507 pKa = 4.05 VIPYY511 pKa = 9.66 KK512 pKa = 10.54 EE513 pKa = 3.96 EE514 pKa = 4.01 SDD516 pKa = 3.61 IVSLIANARR525 pKa = 11.84 VPEE528 pKa = 4.07 EE529 pKa = 3.78 VRR531 pKa = 11.84 DD532 pKa = 3.88 LKK534 pKa = 10.62 KK535 pKa = 7.39 TTGRR539 pKa = 11.84 ARR541 pKa = 11.84 YY542 pKa = 9.49 LFDD545 pKa = 3.59 CARR548 pKa = 11.84 GYY550 pKa = 10.25 MITAAFLHH558 pKa = 6.2 EE559 pKa = 4.58 KK560 pKa = 8.01 TWNICSRR567 pKa = 11.84 LIEE570 pKa = 4.13 EE571 pKa = 4.7 TNTEE575 pKa = 4.52 VICMRR580 pKa = 11.84 VQTGKK585 pKa = 10.76 KK586 pKa = 9.65 VDD588 pKa = 3.52 GEE590 pKa = 4.44 YY591 pKa = 10.9 GGEE594 pKa = 4.19 GPEE597 pKa = 4.2 LVDD600 pKa = 5.52 LVGEE604 pKa = 4.3 DD605 pKa = 4.17 FTWPSSDD612 pKa = 2.77 GWPSIDD618 pKa = 3.59 FCVDD622 pKa = 3.18 VYY624 pKa = 11.06 LSPDD628 pKa = 3.29 NKK630 pKa = 10.48 RR631 pKa = 11.84 GGLWYY636 pKa = 10.19 DD637 pKa = 3.4 CVPALRR643 pKa = 11.84 EE644 pKa = 3.82 KK645 pKa = 10.77 LDD647 pKa = 3.84 EE648 pKa = 4.22 IKK650 pKa = 10.35 RR651 pKa = 11.84 YY652 pKa = 9.66 RR653 pKa = 11.84 SLRR656 pKa = 11.84 DD657 pKa = 3.3 PHH659 pKa = 6.79 HH660 pKa = 6.53 VPPVVSRR667 pKa = 11.84 EE668 pKa = 3.98 VQTTSWAEE676 pKa = 3.74 EE677 pKa = 4.27 VEE679 pKa = 4.4 TEE681 pKa = 4.22 RR682 pKa = 11.84 GRR684 pKa = 11.84 EE685 pKa = 3.83 QAEE688 pKa = 3.71 LAMEE692 pKa = 4.93 RR693 pKa = 11.84 KK694 pKa = 9.54 RR695 pKa = 11.84 DD696 pKa = 3.73 PTVDD700 pKa = 3.06 SEE702 pKa = 4.95 GYY704 pKa = 9.73 LRR706 pKa = 11.84 SFLEE710 pKa = 3.87 EE711 pKa = 3.88 RR712 pKa = 11.84 TPLMKK717 pKa = 9.9 PVKK720 pKa = 9.84 YY721 pKa = 9.56 PKK723 pKa = 10.52 EE724 pKa = 3.84 FVKK727 pKa = 11.04 YY728 pKa = 10.0 KK729 pKa = 10.04 ATTQSVPKK737 pKa = 9.81 EE738 pKa = 3.87 KK739 pKa = 9.95 RR740 pKa = 11.84 IEE742 pKa = 3.89 NAIPEE747 pKa = 4.5 GAEE750 pKa = 3.69 KK751 pKa = 10.18 FHH753 pKa = 7.39 RR754 pKa = 11.84 DD755 pKa = 4.72 FIPLVTNLAPGKK767 pKa = 9.32 CAEE770 pKa = 4.2 VLISLGWRR778 pKa = 11.84 PSPSEE783 pKa = 4.34 SYY785 pKa = 9.12 WEE787 pKa = 4.26 KK788 pKa = 11.16 GPLVPVRR795 pKa = 11.84 DD796 pKa = 4.15 SDD798 pKa = 3.62 WTRR801 pKa = 11.84 AEE803 pKa = 4.1 LDD805 pKa = 3.33 RR806 pKa = 11.84 LKK808 pKa = 10.49 EE809 pKa = 3.97 VPRR812 pKa = 11.84 RR813 pKa = 11.84 EE814 pKa = 3.92 TKK816 pKa = 10.71 SRR818 pKa = 11.84 VKK820 pKa = 10.26 VARR823 pKa = 11.84 DD824 pKa = 3.04 GKK826 pKa = 7.34 TWCEE830 pKa = 3.85 VPDD833 pKa = 4.06 DD834 pKa = 4.24 VKK836 pKa = 11.35 LSDD839 pKa = 3.57 RR840 pKa = 11.84 QVDD843 pKa = 3.94 VVSRR847 pKa = 11.84 VSYY850 pKa = 10.21 SFVNAGHH857 pKa = 6.59 ILDD860 pKa = 3.76 RR861 pKa = 11.84 KK862 pKa = 8.64 YY863 pKa = 9.28 TVLEE867 pKa = 3.98 NSPNPWGVLQVTYY880 pKa = 10.56 RR881 pKa = 11.84 GTGVLVGEE889 pKa = 4.52 GFGVKK894 pKa = 9.48 KK895 pKa = 10.81 DD896 pKa = 3.65 EE897 pKa = 4.18 ATSAVFEE904 pKa = 4.55 LMMEE908 pKa = 4.68 RR909 pKa = 11.84 IMAKK913 pKa = 9.84 EE914 pKa = 3.89 AGPLTSEE921 pKa = 4.66 IVTTEE926 pKa = 3.61 NGEE929 pKa = 4.0 NRR931 pKa = 11.84 ATEE934 pKa = 4.06 SEE936 pKa = 4.11 AARR939 pKa = 11.84 EE940 pKa = 4.02 EE941 pKa = 4.28 EE942 pKa = 4.05 TAEE945 pKa = 4.13 TEE947 pKa = 4.44 RR948 pKa = 11.84 EE949 pKa = 3.98 TEE951 pKa = 3.94 EE952 pKa = 4.4 RR953 pKa = 11.84 NEE955 pKa = 4.22 SGSTQTVAPASEE967 pKa = 4.38 GKK969 pKa = 10.32 DD970 pKa = 3.29 GGNN973 pKa = 3.33
Molecular weight: 110.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.203
IPC2_protein 5.258
IPC_protein 5.232
Toseland 5.169
ProMoST 5.359
Dawson 5.207
Bjellqvist 5.334
Wikipedia 5.067
Rodwell 5.13
Grimsley 5.092
Solomon 5.207
Lehninger 5.169
Nozaki 5.334
DTASelect 5.448
Thurlkill 5.181
EMBOSS 5.105
Sillero 5.41
Patrickios 4.228
IPC_peptide 5.219
IPC2_peptide 5.41
IPC2.peptide.svr19 5.407
Protein with the highest isoelectric point:
>tr|A0A1L3KHU9|A0A1L3KHU9_9VIRU RdRp OS=Beihai permutotetra-like virus 2 OX=1922513 PE=4 SV=1
MM1 pKa = 7.41 VKK3 pKa = 9.41 TEE5 pKa = 3.87 QQRR8 pKa = 11.84 AKK10 pKa = 9.79 QRR12 pKa = 11.84 EE13 pKa = 3.62 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 QRR19 pKa = 11.84 QNARR23 pKa = 11.84 LRR25 pKa = 11.84 KK26 pKa = 9.34 EE27 pKa = 4.21 MNQEE31 pKa = 3.73 APRR34 pKa = 11.84 PLPQRR39 pKa = 11.84 QRR41 pKa = 11.84 GRR43 pKa = 11.84 MGGIKK48 pKa = 10.07 RR49 pKa = 11.84 NCFDD53 pKa = 4.08 GNDD56 pKa = 4.42 LISSVDD62 pKa = 3.49 VPATAKK68 pKa = 10.37 SGTVIARR75 pKa = 11.84 VDD77 pKa = 3.19 ICPSKK82 pKa = 10.96 FPGTSLWRR90 pKa = 11.84 QAQLYY95 pKa = 9.8 ANWKK99 pKa = 7.63 PARR102 pKa = 11.84 KK103 pKa = 9.27 LKK105 pKa = 10.58 IVVQPSAGTTTSGSYY120 pKa = 10.48 IMGWTMDD127 pKa = 3.73 SKK129 pKa = 11.7 LDD131 pKa = 3.78 IPSGDD136 pKa = 3.29 NAVRR140 pKa = 11.84 TVATFEE146 pKa = 3.8 RR147 pKa = 11.84 RR148 pKa = 11.84 QTAKK152 pKa = 10.1 IYY154 pKa = 9.41 EE155 pKa = 4.32 KK156 pKa = 10.93 KK157 pKa = 10.31 EE158 pKa = 3.6 LNIPLKK164 pKa = 9.79 TVQALLFTDD173 pKa = 3.93 SSKK176 pKa = 11.17 EE177 pKa = 3.93 DD178 pKa = 3.37 SDD180 pKa = 3.45 QGSFYY185 pKa = 11.07 LVLSSGIGNLTTGSVVSFNIYY206 pKa = 9.44 IDD208 pKa = 3.67 YY209 pKa = 10.97 DD210 pKa = 3.26 IDD212 pKa = 3.78 FEE214 pKa = 4.68 NRR216 pKa = 11.84 LATPDD221 pKa = 3.44 TSSSFIYY228 pKa = 9.68 PDD230 pKa = 3.81 PGYY233 pKa = 10.91 EE234 pKa = 5.4 SYY236 pKa = 9.17 FTDD239 pKa = 3.64 NGDD242 pKa = 4.26 GITKK246 pKa = 10.57 LSLKK250 pKa = 9.58 HH251 pKa = 6.37 AEE253 pKa = 4.42 GGALVPFHH261 pKa = 6.43 QSIPEE266 pKa = 3.78 AVYY269 pKa = 10.21 EE270 pKa = 4.13 FVGEE274 pKa = 4.05 SLKK277 pKa = 10.69 YY278 pKa = 10.25 CKK280 pKa = 9.87 TGEE283 pKa = 4.17 TSATATGIVTHH294 pKa = 6.41 AVRR297 pKa = 11.84 IRR299 pKa = 11.84 GRR301 pKa = 11.84 PDD303 pKa = 2.6 GALYY307 pKa = 10.82 VFEE310 pKa = 5.45 NNTKK314 pKa = 9.85 ASAYY318 pKa = 10.39 ASTGDD323 pKa = 3.63 EE324 pKa = 4.72 DD325 pKa = 4.25 NCITYY330 pKa = 10.04 VAAGPWVSPTGAGFKK345 pKa = 10.36 QISSPSALYY354 pKa = 10.75 SNRR357 pKa = 11.84 LNN359 pKa = 3.59
Molecular weight: 39.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.677
IPC2_protein 8.624
IPC_protein 8.551
Toseland 9.077
ProMoST 8.99
Dawson 9.428
Bjellqvist 9.253
Wikipedia 9.619
Rodwell 9.589
Grimsley 9.502
Solomon 9.487
Lehninger 9.443
Nozaki 9.297
DTASelect 9.18
Thurlkill 9.268
EMBOSS 9.545
Sillero 9.414
Patrickios 4.647
IPC_peptide 9.472
IPC2_peptide 7.922
IPC2.peptide.svr19 7.721
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1332
359
973
666.0
74.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.381 ± 0.805
1.877 ± 0.362
6.081 ± 0.242
8.859 ± 1.957
4.054 ± 0.205
7.282 ± 0.245
1.276 ± 0.209
3.754 ± 0.862
7.207 ± 0.644
6.757 ± 0.298
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.652 ± 0.123
4.129 ± 0.155
5.105 ± 0.089
3.078 ± 0.654
6.456 ± 0.241
6.757 ± 1.156
6.456 ± 0.637
7.808 ± 0.929
1.502 ± 0.184
3.529 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here