Capybara microvirus Cap1_SP_90
Average proteome isoelectric point is 5.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W418|A0A4P8W418_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_90 OX=2584797 PE=4 SV=1
MM1 pKa = 7.37 FPTVEE6 pKa = 4.44 KK7 pKa = 9.41 FQCSRR12 pKa = 11.84 GCPVYY17 pKa = 10.05 ATVDD21 pKa = 3.62 RR22 pKa = 11.84 EE23 pKa = 4.18 VLIDD27 pKa = 3.74 SDD29 pKa = 4.1 GLAVPIIEE37 pKa = 4.41 KK38 pKa = 9.52 TVIDD42 pKa = 3.93 MTTEE46 pKa = 3.82 EE47 pKa = 4.69 SIKK50 pKa = 10.99 SLGTGLSLKK59 pKa = 10.43 DD60 pKa = 3.45 ALEE63 pKa = 4.32 AGISIDD69 pKa = 3.75 RR70 pKa = 11.84 VNTQVLSTSRR80 pKa = 11.84 ADD82 pKa = 3.55 NLEE85 pKa = 4.16 KK86 pKa = 10.78 YY87 pKa = 10.41 VEE89 pKa = 5.02 LINKK93 pKa = 9.81 DD94 pKa = 2.92 IEE96 pKa = 4.31 RR97 pKa = 11.84 TLNEE101 pKa = 4.11 SKK103 pKa = 11.05
Molecular weight: 11.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.705
IPC2_protein 4.647
IPC_protein 4.507
Toseland 4.342
ProMoST 4.609
Dawson 4.444
Bjellqvist 4.596
Wikipedia 4.317
Rodwell 4.342
Grimsley 4.253
Solomon 4.444
Lehninger 4.393
Nozaki 4.558
DTASelect 4.698
Thurlkill 4.342
EMBOSS 4.329
Sillero 4.609
Patrickios 4.151
IPC_peptide 4.444
IPC2_peptide 4.596
IPC2.peptide.svr19 4.581
Protein with the highest isoelectric point:
>tr|A0A4V1FVN4|A0A4V1FVN4_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_90 OX=2584797 PE=4 SV=1
MM1 pKa = 7.97 CYY3 pKa = 9.99 NPRR6 pKa = 11.84 VIRR9 pKa = 11.84 NPTKK13 pKa = 10.83 DD14 pKa = 3.98 LVPTDD19 pKa = 3.68 ALQITVSCGEE29 pKa = 4.08 CEE31 pKa = 3.74 EE32 pKa = 4.6 CRR34 pKa = 11.84 NHH36 pKa = 6.65 RR37 pKa = 11.84 RR38 pKa = 11.84 NEE40 pKa = 4.08 MVFRR44 pKa = 11.84 TYY46 pKa = 10.9 EE47 pKa = 4.25 QMLEE51 pKa = 4.07 DD52 pKa = 3.64 QKK54 pKa = 11.4 KK55 pKa = 10.83 GYY57 pKa = 8.55 IQCFFTLTYY66 pKa = 11.07 NDD68 pKa = 3.82 DD69 pKa = 4.0 CLPHH73 pKa = 6.35 FKK75 pKa = 10.34 CSSYY79 pKa = 10.95 NIPCFSPDD87 pKa = 4.06 DD88 pKa = 3.47 ISAFRR93 pKa = 11.84 GHH95 pKa = 6.43 LQDD98 pKa = 4.29 YY99 pKa = 10.69 LLDD102 pKa = 4.23 RR103 pKa = 11.84 YY104 pKa = 10.07 GIKK107 pKa = 10.38 SSTYY111 pKa = 9.97 LICAEE116 pKa = 4.38 YY117 pKa = 10.75 GSNTQRR123 pKa = 11.84 PHH125 pKa = 4.14 YY126 pKa = 9.23 HH127 pKa = 7.15 CYY129 pKa = 9.2 FACPSKK135 pKa = 11.1 GVITPRR141 pKa = 11.84 FTKK144 pKa = 9.29 KK145 pKa = 9.17 TGITSEE151 pKa = 4.36 SNLPVLTGSILHH163 pKa = 6.49 SIVKK167 pKa = 9.27 EE168 pKa = 3.66 CWTYY172 pKa = 11.25 GFLFPRR178 pKa = 11.84 FPQGGRR184 pKa = 11.84 DD185 pKa = 3.39 STGYY189 pKa = 7.94 NHH191 pKa = 7.33 KK192 pKa = 10.46 PFVLTGDD199 pKa = 3.33 VTGAAKK205 pKa = 10.61 YY206 pKa = 8.54 IAKK209 pKa = 9.49 YY210 pKa = 8.82 VCKK213 pKa = 10.44 DD214 pKa = 3.17 LALLEE219 pKa = 4.66 RR220 pKa = 11.84 PDD222 pKa = 3.56 VQQFIAEE229 pKa = 4.87 FKK231 pKa = 10.44 NKK233 pKa = 10.27 LNGKK237 pKa = 8.82 HH238 pKa = 6.92 DD239 pKa = 3.65 IYY241 pKa = 11.51 KK242 pKa = 10.34 KK243 pKa = 8.48 FQKK246 pKa = 10.19 VSSLMRR252 pKa = 11.84 CSQGFGKK259 pKa = 9.91 CICDD263 pKa = 3.38 KK264 pKa = 10.94 VKK266 pKa = 10.98 SVDD269 pKa = 3.93 DD270 pKa = 3.79 IKK272 pKa = 11.61 NGVTTNLYY280 pKa = 5.9 PTHH283 pKa = 7.05 KK284 pKa = 9.96 IAVPQYY290 pKa = 9.64 IVRR293 pKa = 11.84 KK294 pKa = 6.81 LTRR297 pKa = 11.84 VAHH300 pKa = 5.68 YY301 pKa = 9.97 IKK303 pKa = 10.55 EE304 pKa = 4.16 DD305 pKa = 3.32 ASGFKK310 pKa = 10.1 YY311 pKa = 10.54 LKK313 pKa = 10.06 PKK315 pKa = 9.84 VRR317 pKa = 11.84 YY318 pKa = 8.9 EE319 pKa = 3.59 LTEE322 pKa = 4.12 FGKK325 pKa = 10.43 EE326 pKa = 4.03 VYY328 pKa = 10.01 KK329 pKa = 10.82 EE330 pKa = 4.24 SIKK333 pKa = 10.81 HH334 pKa = 5.13 QIKK337 pKa = 10.61 NLTKK341 pKa = 10.41 SLHH344 pKa = 6.5 DD345 pKa = 4.04 WNPSIDD351 pKa = 3.71 AQSIATYY358 pKa = 10.42 AVVYY362 pKa = 9.34 RR363 pKa = 11.84 NRR365 pKa = 11.84 MNPRR369 pKa = 11.84 HH370 pKa = 5.5 STIKK374 pKa = 10.74 DD375 pKa = 3.09 AFTCEE380 pKa = 3.81 LTSADD385 pKa = 4.01 FFTGLEE391 pKa = 4.11 SLSRR395 pKa = 11.84 MMSKK399 pKa = 10.66 ALPYY403 pKa = 10.45 KK404 pKa = 10.03 FAHH407 pKa = 6.65 LDD409 pKa = 3.72 YY410 pKa = 11.2 VKK412 pKa = 10.61 LPQSPLVNDD421 pKa = 3.67 DD422 pKa = 3.57 VYY424 pKa = 11.45 YY425 pKa = 10.93 HH426 pKa = 7.34 LDD428 pKa = 2.94 LGYY431 pKa = 10.64 RR432 pKa = 11.84 FNDD435 pKa = 4.39 FKK437 pKa = 11.23 CFEE440 pKa = 4.58 GFDD443 pKa = 4.89 DD444 pKa = 5.22 IYY446 pKa = 11.45 DD447 pKa = 3.81 SYY449 pKa = 12.2 VEE451 pKa = 4.04 YY452 pKa = 11.11 NNKK455 pKa = 9.3 KK456 pKa = 10.11 KK457 pKa = 10.76 KK458 pKa = 10.39 EE459 pKa = 3.9 NLEE462 pKa = 3.91 LKK464 pKa = 9.95 AAHH467 pKa = 5.98 AQYY470 pKa = 10.55 IKK472 pKa = 10.57 SLKK475 pKa = 9.05 EE476 pKa = 4.04 HH477 pKa = 6.96 FSHH480 pKa = 7.42 TEE482 pKa = 3.49 NNNNNKK488 pKa = 9.05 YY489 pKa = 10.27 RR490 pKa = 11.84 SLCFRR495 pKa = 11.84 QQ496 pKa = 3.3
Molecular weight: 57.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.081
IPC2_protein 8.156
IPC_protein 7.951
Toseland 7.775
ProMoST 8.521
Dawson 8.741
Bjellqvist 8.96
Wikipedia 8.741
Rodwell 8.814
Grimsley 7.805
Solomon 8.799
Lehninger 8.785
Nozaki 9.107
DTASelect 8.741
Thurlkill 8.799
EMBOSS 8.916
Sillero 9.077
Patrickios 3.923
IPC_peptide 8.785
IPC2_peptide 7.849
IPC2.peptide.svr19 8.031
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1739
103
733
434.8
48.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.383 ± 1.585
1.725 ± 0.771
6.21 ± 0.238
5.003 ± 0.496
4.485 ± 0.538
5.808 ± 0.707
1.783 ± 0.674
5.463 ± 0.579
6.153 ± 1.334
8.338 ± 0.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.384
6.038 ± 0.557
4.485 ± 0.542
3.968 ± 0.584
4.428 ± 0.169
8.626 ± 0.718
6.671 ± 0.5
5.693 ± 0.319
0.805 ± 0.238
5.578 ± 0.624
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here