Amino acid dipepetide frequency for Clostridium sp. W14A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.126AlaAla: 10.126 ± 0.134
1.346AlaCys: 1.346 ± 0.04
4.767AlaAsp: 4.767 ± 0.074
5.954AlaGlu: 5.954 ± 0.095
3.475AlaPhe: 3.475 ± 0.058
7.232AlaGly: 7.232 ± 0.098
1.251AlaHis: 1.251 ± 0.038
4.619AlaIle: 4.619 ± 0.081
4.622AlaLys: 4.622 ± 0.064
8.334AlaLeu: 8.334 ± 0.096
2.34AlaMet: 2.34 ± 0.044
2.484AlaAsn: 2.484 ± 0.047
2.722AlaPro: 2.722 ± 0.052
2.798AlaGln: 2.798 ± 0.055
3.978AlaArg: 3.978 ± 0.069
4.771AlaSer: 4.771 ± 0.084
3.272AlaThr: 3.272 ± 0.062
8.021AlaVal: 8.021 ± 0.096
0.611AlaTrp: 0.611 ± 0.026
2.488AlaTyr: 2.488 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
1.48CysAla: 1.48 ± 0.034
0.335CysCys: 0.335 ± 0.019
0.897CysAsp: 0.897 ± 0.031
0.925CysGlu: 0.925 ± 0.03
0.651CysPhe: 0.651 ± 0.024
1.825CysGly: 1.825 ± 0.05
0.294CysHis: 0.294 ± 0.018
0.901CysIle: 0.901 ± 0.028
0.704CysLys: 0.704 ± 0.027
1.268CysLeu: 1.268 ± 0.034
0.416CysMet: 0.416 ± 0.021
0.517CysAsn: 0.517 ± 0.022
0.713CysPro: 0.713 ± 0.029
0.366CysGln: 0.366 ± 0.019
1.032CysArg: 1.032 ± 0.033
1.08CysSer: 1.08 ± 0.032
0.727CysThr: 0.727 ± 0.028
1.14CysVal: 1.14 ± 0.035
0.127CysTrp: 0.127 ± 0.01
0.521CysTyr: 0.521 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.358AspAla: 4.358 ± 0.065
0.932AspCys: 0.932 ± 0.033
2.575AspAsp: 2.575 ± 0.06
3.869AspGlu: 3.869 ± 0.071
2.606AspPhe: 2.606 ± 0.052
4.622AspGly: 4.622 ± 0.061
0.923AspHis: 0.923 ± 0.036
3.279AspIle: 3.279 ± 0.061
2.523AspLys: 2.523 ± 0.058
4.959AspLeu: 4.959 ± 0.07
1.361AspMet: 1.361 ± 0.037
1.684AspAsn: 1.684 ± 0.044
2.307AspPro: 2.307 ± 0.052
1.439AspGln: 1.439 ± 0.035
3.289AspArg: 3.289 ± 0.051
3.636AspSer: 3.636 ± 0.064
2.792AspThr: 2.792 ± 0.078
3.331AspVal: 3.331 ± 0.056
0.581AspTrp: 0.581 ± 0.026
2.263AspTyr: 2.263 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
4.963GluAla: 4.963 ± 0.085
0.726GluCys: 0.726 ± 0.028
3.123GluAsp: 3.123 ± 0.055
4.854GluGlu: 4.854 ± 0.087
2.404GluPhe: 2.404 ± 0.049
3.449GluGly: 3.449 ± 0.066
1.181GluHis: 1.181 ± 0.033
4.952GluIle: 4.952 ± 0.066
5.332GluLys: 5.332 ± 0.079
6.233GluLeu: 6.233 ± 0.089
1.837GluMet: 1.837 ± 0.042
3.528GluAsn: 3.528 ± 0.06
2.198GluPro: 2.198 ± 0.056
2.867GluGln: 2.867 ± 0.062
3.639GluArg: 3.639 ± 0.072
3.526GluSer: 3.526 ± 0.059
3.469GluThr: 3.469 ± 0.068
3.355GluVal: 3.355 ± 0.062
0.584GluTrp: 0.584 ± 0.026
2.187GluTyr: 2.187 ± 0.046
0.0GluXaa: 0.0 ± 0.0
Phe
3.294PheAla: 3.294 ± 0.065
0.816PheCys: 0.816 ± 0.025
2.376PheAsp: 2.376 ± 0.05
2.342PheGlu: 2.342 ± 0.044
2.053PhePhe: 2.053 ± 0.054
3.403PheGly: 3.403 ± 0.063
0.93PheHis: 0.93 ± 0.03
2.44PheIle: 2.44 ± 0.051
1.529PheLys: 1.529 ± 0.038
4.664PheLeu: 4.664 ± 0.084
0.986PheMet: 0.986 ± 0.026
1.423PheAsn: 1.423 ± 0.037
1.637PhePro: 1.637 ± 0.038
1.607PheGln: 1.607 ± 0.04
2.289PheArg: 2.289 ± 0.049
3.571PheSer: 3.571 ± 0.06
2.48PheThr: 2.48 ± 0.045
2.709PheVal: 2.709 ± 0.053
0.437PheTrp: 0.437 ± 0.022
1.614PheTyr: 1.614 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
6.069GlyAla: 6.069 ± 0.084
1.433GlyCys: 1.433 ± 0.042
3.828GlyAsp: 3.828 ± 0.08
4.46GlyGlu: 4.46 ± 0.072
3.428GlyPhe: 3.428 ± 0.061
6.201GlyGly: 6.201 ± 0.105
1.225GlyHis: 1.225 ± 0.035
5.941GlyIle: 5.941 ± 0.078
5.198GlyLys: 5.198 ± 0.069
6.792GlyLeu: 6.792 ± 0.085
2.424GlyMet: 2.424 ± 0.045
2.963GlyAsn: 2.963 ± 0.055
1.98GlyPro: 1.98 ± 0.124
2.111GlyGln: 2.111 ± 0.045
3.897GlyArg: 3.897 ± 0.066
4.808GlySer: 4.808 ± 0.077
4.574GlyThr: 4.574 ± 0.074
5.406GlyVal: 5.406 ± 0.076
0.752GlyTrp: 0.752 ± 0.027
2.993GlyTyr: 2.993 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
1.279HisAla: 1.279 ± 0.037
0.344HisCys: 0.344 ± 0.019
0.819HisAsp: 0.819 ± 0.028
0.912HisGlu: 0.912 ± 0.03
0.781HisPhe: 0.781 ± 0.026
1.351HisGly: 1.351 ± 0.036
0.367HisHis: 0.367 ± 0.017
1.144HisIle: 1.144 ± 0.034
0.739HisLys: 0.739 ± 0.026
1.555HisLeu: 1.555 ± 0.041
0.46HisMet: 0.46 ± 0.018
0.613HisAsn: 0.613 ± 0.024
0.978HisPro: 0.978 ± 0.026
0.523HisGln: 0.523 ± 0.023
0.989HisArg: 0.989 ± 0.029
1.093HisSer: 1.093 ± 0.035
0.978HisThr: 0.978 ± 0.027
1.095HisVal: 1.095 ± 0.03
0.183HisTrp: 0.183 ± 0.014
0.651HisTyr: 0.651 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.633IleAla: 5.633 ± 0.077
1.197IleCys: 1.197 ± 0.037
3.715IleAsp: 3.715 ± 0.057
3.581IleGlu: 3.581 ± 0.065
2.599IlePhe: 2.599 ± 0.056
4.937IleGly: 4.937 ± 0.081
1.186IleHis: 1.186 ± 0.037
3.683IleIle: 3.683 ± 0.078
2.983IleLys: 2.983 ± 0.053
6.901IleLeu: 6.901 ± 0.09
1.499IleMet: 1.499 ± 0.034
2.32IleAsn: 2.32 ± 0.055
3.257IlePro: 3.257 ± 0.066
2.078IleGln: 2.078 ± 0.042
3.989IleArg: 3.989 ± 0.06
4.905IleSer: 4.905 ± 0.08
3.599IleThr: 3.599 ± 0.063
4.407IleVal: 4.407 ± 0.073
0.522IleTrp: 0.522 ± 0.025
2.087IleTyr: 2.087 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
4.826LysAla: 4.826 ± 0.078
0.602LysCys: 0.602 ± 0.025
3.049LysAsp: 3.049 ± 0.06
4.119LysGlu: 4.119 ± 0.073
1.674LysPhe: 1.674 ± 0.044
3.55LysGly: 3.55 ± 0.062
0.881LysHis: 0.881 ± 0.031
4.468LysIle: 4.468 ± 0.064
4.65LysLys: 4.65 ± 0.065
4.853LysLeu: 4.853 ± 0.067
1.754LysMet: 1.754 ± 0.041
3.133LysAsn: 3.133 ± 0.056
2.166LysPro: 2.166 ± 0.048
2.148LysGln: 2.148 ± 0.043
3.005LysArg: 3.005 ± 0.055
3.693LysSer: 3.693 ± 0.06
3.7LysThr: 3.7 ± 0.063
3.26LysVal: 3.26 ± 0.057
0.503LysTrp: 0.503 ± 0.021
2.151LysTyr: 2.151 ± 0.051
0.0LysXaa: 0.0 ± 0.0
Leu
7.703LeuAla: 7.703 ± 0.096
1.834LeuCys: 1.834 ± 0.044
4.967LeuAsp: 4.967 ± 0.083
5.496LeuGlu: 5.496 ± 0.086
4.501LeuPhe: 4.501 ± 0.081
6.384LeuGly: 6.384 ± 0.084
1.564LeuHis: 1.564 ± 0.038
6.335LeuIle: 6.335 ± 0.096
5.943LeuLys: 5.943 ± 0.075
9.841LeuLeu: 9.841 ± 0.136
2.592LeuMet: 2.592 ± 0.052
4.055LeuAsn: 4.055 ± 0.067
4.358LeuPro: 4.358 ± 0.064
2.817LeuGln: 2.817 ± 0.048
5.046LeuArg: 5.046 ± 0.082
7.738LeuSer: 7.738 ± 0.088
5.634LeuThr: 5.634 ± 0.076
5.601LeuVal: 5.601 ± 0.082
0.852LeuTrp: 0.852 ± 0.032
3.172LeuTyr: 3.172 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
2.204MetAla: 2.204 ± 0.048
0.276MetCys: 0.276 ± 0.017
1.528MetAsp: 1.528 ± 0.041
1.943MetGlu: 1.943 ± 0.041
0.943MetPhe: 0.943 ± 0.034
1.835MetGly: 1.835 ± 0.046
0.358MetHis: 0.358 ± 0.017
1.866MetIle: 1.866 ± 0.04
2.277MetLys: 2.277 ± 0.05
2.659MetLeu: 2.659 ± 0.05
0.787MetMet: 0.787 ± 0.03
1.408MetAsn: 1.408 ± 0.035
1.193MetPro: 1.193 ± 0.034
0.876MetGln: 0.876 ± 0.027
1.399MetArg: 1.399 ± 0.042
1.538MetSer: 1.538 ± 0.043
1.646MetThr: 1.646 ± 0.036
1.619MetVal: 1.619 ± 0.04
0.189MetTrp: 0.189 ± 0.013
0.578MetTyr: 0.578 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.184AsnAla: 3.184 ± 0.057
0.618AsnCys: 0.618 ± 0.025
1.902AsnAsp: 1.902 ± 0.042
2.216AsnGlu: 2.216 ± 0.05
1.559AsnPhe: 1.559 ± 0.038
3.561AsnGly: 3.561 ± 0.068
0.718AsnHis: 0.718 ± 0.025
2.683AsnIle: 2.683 ± 0.05
1.745AsnLys: 1.745 ± 0.046
3.931AsnLeu: 3.931 ± 0.058
0.992AsnMet: 0.992 ± 0.03
1.355AsnAsn: 1.355 ± 0.042
2.139AsnPro: 2.139 ± 0.048
1.398AsnGln: 1.398 ± 0.039
2.403AsnArg: 2.403 ± 0.044
2.633AsnSer: 2.633 ± 0.049
2.041AsnThr: 2.041 ± 0.047
2.543AsnVal: 2.543 ± 0.046
0.398AsnTrp: 0.398 ± 0.02
1.517AsnTyr: 1.517 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
3.543ProAla: 3.543 ± 0.072
0.59ProCys: 0.59 ± 0.025
2.879ProAsp: 2.879 ± 0.058
3.591ProGlu: 3.591 ± 0.074
1.8ProPhe: 1.8 ± 0.036
2.949ProGly: 2.949 ± 0.059
0.65ProHis: 0.65 ± 0.022
2.096ProIle: 2.096 ± 0.042
2.077ProLys: 2.077 ± 0.044
3.219ProLeu: 3.219 ± 0.056
0.983ProMet: 0.983 ± 0.032
1.449ProAsn: 1.449 ± 0.04
1.272ProPro: 1.272 ± 0.043
1.4ProGln: 1.4 ± 0.037
1.507ProArg: 1.507 ± 0.041
2.364ProSer: 2.364 ± 0.046
1.896ProThr: 1.896 ± 0.108
3.584ProVal: 3.584 ± 0.06
0.345ProTrp: 0.345 ± 0.019
1.391ProTyr: 1.391 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.801GlnAla: 2.801 ± 0.053
0.383GlnCys: 0.383 ± 0.019
1.53GlnAsp: 1.53 ± 0.043
2.175GlnGlu: 2.175 ± 0.045
1.194GlnPhe: 1.194 ± 0.031
2.0GlnGly: 2.0 ± 0.045
0.475GlnHis: 0.475 ± 0.023
2.474GlnIle: 2.474 ± 0.054
2.791GlnLys: 2.791 ± 0.058
2.767GlnLeu: 2.767 ± 0.051
1.078GlnMet: 1.078 ± 0.037
1.899GlnAsn: 1.899 ± 0.043
1.138GlnPro: 1.138 ± 0.035
1.237GlnGln: 1.237 ± 0.041
1.638GlnArg: 1.638 ± 0.041
2.07GlnSer: 2.07 ± 0.048
1.865GlnThr: 1.865 ± 0.044
1.979GlnVal: 1.979 ± 0.045
0.294GlnTrp: 0.294 ± 0.017
1.229GlnTyr: 1.229 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
3.933ArgAla: 3.933 ± 0.074
0.776ArgCys: 0.776 ± 0.026
2.618ArgAsp: 2.618 ± 0.057
4.241ArgGlu: 4.241 ± 0.079
2.461ArgPhe: 2.461 ± 0.055
3.392ArgGly: 3.392 ± 0.069
0.907ArgHis: 0.907 ± 0.031
3.779ArgIle: 3.779 ± 0.063
3.489ArgLys: 3.489 ± 0.069
5.44ArgLeu: 5.44 ± 0.086
1.595ArgMet: 1.595 ± 0.043
2.224ArgAsn: 2.224 ± 0.047
1.93ArgPro: 1.93 ± 0.048
2.019ArgGln: 2.019 ± 0.048
3.463ArgArg: 3.463 ± 0.066
3.043ArgSer: 3.043 ± 0.065
2.6ArgThr: 2.6 ± 0.046
3.384ArgVal: 3.384 ± 0.055
0.496ArgTrp: 0.496 ± 0.022
2.009ArgTyr: 2.009 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
5.961SerAla: 5.961 ± 0.082
1.021SerCys: 1.021 ± 0.034
3.618SerAsp: 3.618 ± 0.06
4.048SerGlu: 4.048 ± 0.064
2.983SerPhe: 2.983 ± 0.062
6.483SerGly: 6.483 ± 0.094
1.026SerHis: 1.026 ± 0.03
4.098SerIle: 4.098 ± 0.072
3.157SerLys: 3.157 ± 0.064
6.188SerLeu: 6.188 ± 0.08
1.882SerMet: 1.882 ± 0.043
2.167SerAsn: 2.167 ± 0.051
2.507SerPro: 2.507 ± 0.047
2.066SerGln: 2.066 ± 0.042
3.413SerArg: 3.413 ± 0.048
4.754SerSer: 4.754 ± 0.108
3.043SerThr: 3.043 ± 0.057
5.296SerVal: 5.296 ± 0.074
0.546SerTrp: 0.546 ± 0.023
2.182SerTyr: 2.182 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
5.463ThrAla: 5.463 ± 0.077
0.632ThrCys: 0.632 ± 0.023
3.137ThrAsp: 3.137 ± 0.062
3.191ThrGlu: 3.191 ± 0.051
2.108ThrPhe: 2.108 ± 0.047
5.177ThrGly: 5.177 ± 0.19
0.909ThrHis: 0.909 ± 0.032
3.228ThrIle: 3.228 ± 0.061
2.559ThrLys: 2.559 ± 0.048
5.166ThrLeu: 5.166 ± 0.076
1.342ThrMet: 1.342 ± 0.034
1.753ThrAsn: 1.753 ± 0.048
2.403ThrPro: 2.403 ± 0.049
1.58ThrGln: 1.58 ± 0.043
2.243ThrArg: 2.243 ± 0.05
2.879ThrSer: 2.879 ± 0.058
2.501ThrThr: 2.501 ± 0.057
5.136ThrVal: 5.136 ± 0.096
0.398ThrTrp: 0.398 ± 0.019
1.616ThrTyr: 1.616 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
5.148ValAla: 5.148 ± 0.088
1.313ValCys: 1.313 ± 0.036
3.461ValAsp: 3.461 ± 0.048
3.922ValGlu: 3.922 ± 0.064
3.25ValPhe: 3.25 ± 0.064
4.724ValGly: 4.724 ± 0.074
1.076ValHis: 1.076 ± 0.031
4.52ValIle: 4.52 ± 0.077
3.759ValLys: 3.759 ± 0.064
7.232ValLeu: 7.232 ± 0.086
1.823ValMet: 1.823 ± 0.044
2.68ValAsn: 2.68 ± 0.055
3.034ValPro: 3.034 ± 0.05
2.056ValGln: 2.056 ± 0.046
3.757ValArg: 3.757 ± 0.065
5.373ValSer: 5.373 ± 0.075
4.306ValThr: 4.306 ± 0.08
4.664ValVal: 4.664 ± 0.08
0.636ValTrp: 0.636 ± 0.025
2.304ValTyr: 2.304 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.619TrpAla: 0.619 ± 0.025
0.153TrpCys: 0.153 ± 0.013
0.498TrpAsp: 0.498 ± 0.023
0.495TrpGlu: 0.495 ± 0.02
0.417TrpPhe: 0.417 ± 0.018
0.632TrpGly: 0.632 ± 0.027
0.207TrpHis: 0.207 ± 0.017
0.615TrpIle: 0.615 ± 0.025
0.596TrpLys: 0.596 ± 0.025
0.873TrpLeu: 0.873 ± 0.028
0.286TrpMet: 0.286 ± 0.017
0.522TrpAsn: 0.522 ± 0.021
0.24TrpPro: 0.24 ± 0.015
0.337TrpGln: 0.337 ± 0.02
0.474TrpArg: 0.474 ± 0.022
0.519TrpSer: 0.519 ± 0.023
0.425TrpThr: 0.425 ± 0.019
0.561TrpVal: 0.561 ± 0.022
0.114TrpTrp: 0.114 ± 0.012
0.293TrpTyr: 0.293 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.626TyrAla: 2.626 ± 0.054
0.592TyrCys: 0.592 ± 0.02
2.046TyrAsp: 2.046 ± 0.046
2.074TyrGlu: 2.074 ± 0.045
1.712TyrPhe: 1.712 ± 0.048
2.774TyrGly: 2.774 ± 0.056
0.726TyrHis: 0.726 ± 0.027
1.956TyrIle: 1.956 ± 0.049
1.513TyrLys: 1.513 ± 0.041
3.556TyrLeu: 3.556 ± 0.058
0.681TyrMet: 0.681 ± 0.025
1.36TyrAsn: 1.36 ± 0.043
1.413TyrPro: 1.413 ± 0.039
1.296TyrGln: 1.296 ± 0.044
2.351TyrArg: 2.351 ± 0.06
2.445TyrSer: 2.445 ± 0.05
1.875TyrThr: 1.875 ± 0.044
1.942TyrVal: 1.942 ± 0.036
0.309TyrTrp: 0.309 ± 0.017
1.418TyrTyr: 1.418 ± 0.048
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3645 proteins (1085271 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski