Streptomyces sp. So13.3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7990 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5R9M9T9|A0A5R9M9T9_9ACTN FAD-dependent oxidoreductase OS=Streptomyces sp. So13.3 OX=2136173 GN=C8250_002085 PE=3 SV=1
MM1 pKa = 7.45NNTTGAAYY9 pKa = 7.92EE10 pKa = 4.17AQLEE14 pKa = 4.37FHH16 pKa = 7.55DD17 pKa = 4.63CTPGQPAQSVSRR29 pKa = 11.84HH30 pKa = 4.96ISVAPGASYY39 pKa = 10.83FDD41 pKa = 4.3EE42 pKa = 4.35FHH44 pKa = 6.9LAATAPRR51 pKa = 11.84ITHH54 pKa = 5.8TMCGTLVQDD63 pKa = 3.63GGGPVTTFSDD73 pKa = 5.15PIHH76 pKa = 6.91PPSSAPPVPSDD87 pKa = 3.92TPDD90 pKa = 3.49SPDD93 pKa = 3.69TPDD96 pKa = 5.03SPDD99 pKa = 3.47TPDD102 pKa = 3.52TPDD105 pKa = 3.21VSEE108 pKa = 5.3GPATSDD114 pKa = 3.54ASTPP118 pKa = 3.51

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5R9MBQ3|A0A5R9MBQ3_9ACTN Abasic site processing protein OS=Streptomyces sp. So13.3 OX=2136173 GN=C8250_00565 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84VKK38 pKa = 10.66GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7990

0

7990

2631408

29

7615

329.3

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.572 ± 0.036

0.809 ± 0.007

5.943 ± 0.023

5.207 ± 0.031

2.764 ± 0.015

9.433 ± 0.03

2.298 ± 0.013

3.439 ± 0.018

2.15 ± 0.024

10.372 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.757 ± 0.012

1.962 ± 0.016

6.075 ± 0.025

2.961 ± 0.016

7.649 ± 0.032

5.333 ± 0.024

6.375 ± 0.031

8.228 ± 0.026

1.55 ± 0.011

2.124 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski