Molossus molossus circovirus 3
Average proteome isoelectric point is 7.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I2MP65|A0A2I2MP65_9CIRC Capsid OS=Molossus molossus circovirus 3 OX=1959844 GN=Cap PE=4 SV=1
MM1 pKa = 7.39 QSRR4 pKa = 11.84 HH5 pKa = 5.0 YY6 pKa = 10.78 CFTLNNPKK14 pKa = 10.28 EE15 pKa = 4.65 LIDD18 pKa = 3.88 FTTIPHH24 pKa = 5.06 VRR26 pKa = 11.84 YY27 pKa = 9.79 AIYY30 pKa = 10.12 QEE32 pKa = 4.66 EE33 pKa = 4.25 IGEE36 pKa = 4.24 SGNYY40 pKa = 8.94 HH41 pKa = 5.03 FQGYY45 pKa = 9.72 IEE47 pKa = 4.5 FDD49 pKa = 3.07 RR50 pKa = 11.84 PTRR53 pKa = 11.84 LGFLQRR59 pKa = 11.84 HH60 pKa = 5.7 LNGGHH65 pKa = 6.03 FEE67 pKa = 4.1 LRR69 pKa = 11.84 RR70 pKa = 11.84 GSRR73 pKa = 11.84 DD74 pKa = 3.0 EE75 pKa = 3.74 ARR77 pKa = 11.84 EE78 pKa = 3.81 YY79 pKa = 10.66 CRR81 pKa = 11.84 KK82 pKa = 9.48 LDD84 pKa = 3.64 SRR86 pKa = 11.84 IGEE89 pKa = 4.14 VFEE92 pKa = 5.08 YY93 pKa = 11.07 GDD95 pKa = 4.0 WDD97 pKa = 3.29 QGGQGVRR104 pKa = 11.84 TDD106 pKa = 4.04 FNALHH111 pKa = 6.07 EE112 pKa = 4.65 LVRR115 pKa = 11.84 QGTPTSEE122 pKa = 5.15 IMDD125 pKa = 3.51 QMPSMFYY132 pKa = 9.96 RR133 pKa = 11.84 YY134 pKa = 9.66 HH135 pKa = 7.65 SGIGKK140 pKa = 9.02 AQLLCVQDD148 pKa = 4.17 RR149 pKa = 11.84 DD150 pKa = 3.71 WPTKK154 pKa = 10.34 IIVLYY159 pKa = 9.97 GASGTGKK166 pKa = 10.2 SRR168 pKa = 11.84 WAHH171 pKa = 6.41 DD172 pKa = 3.72 YY173 pKa = 10.31 CHH175 pKa = 7.23 PSHH178 pKa = 6.51 QYY180 pKa = 8.55 WVSNTKK186 pKa = 9.54 WFDD189 pKa = 3.3 GYY191 pKa = 10.77 EE192 pKa = 4.01 FQPVVVLDD200 pKa = 4.77 DD201 pKa = 3.86 FRR203 pKa = 11.84 GWLPLHH209 pKa = 5.84 QLLRR213 pKa = 11.84 LCDD216 pKa = 3.96 RR217 pKa = 11.84 YY218 pKa = 10.41 PYY220 pKa = 8.73 TVEE223 pKa = 3.51 WKK225 pKa = 9.88 GGSRR229 pKa = 11.84 KK230 pKa = 8.16 FTAHH234 pKa = 6.34 VIIITSTRR242 pKa = 11.84 RR243 pKa = 11.84 PDD245 pKa = 2.7 EE246 pKa = 4.04 WYY248 pKa = 9.92 NWEE251 pKa = 4.53 EE252 pKa = 3.9 LHH254 pKa = 7.05 EE255 pKa = 5.37 DD256 pKa = 3.26 PAQLFRR262 pKa = 11.84 RR263 pKa = 11.84 IDD265 pKa = 3.5 EE266 pKa = 4.37 FLGRR270 pKa = 11.84 TPRR273 pKa = 11.84 RR274 pKa = 11.84 SRR276 pKa = 11.84 TALSEE281 pKa = 4.05 DD282 pKa = 3.48 RR283 pKa = 4.83
Molecular weight: 33.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.297
IPC2_protein 6.224
IPC_protein 6.364
Toseland 6.693
ProMoST 6.81
Dawson 6.722
Bjellqvist 6.678
Wikipedia 6.751
Rodwell 6.722
Grimsley 6.839
Solomon 6.737
Lehninger 6.737
Nozaki 6.985
DTASelect 7.161
Thurlkill 7.19
EMBOSS 7.176
Sillero 7.117
Patrickios 4.075
IPC_peptide 6.751
IPC2_peptide 7.015
IPC2.peptide.svr19 6.896
Protein with the highest isoelectric point:
>tr|A0A2I2MP65|A0A2I2MP65_9CIRC Capsid OS=Molossus molossus circovirus 3 OX=1959844 GN=Cap PE=4 SV=1
MM1 pKa = 7.36 RR2 pKa = 11.84 RR3 pKa = 11.84 KK4 pKa = 9.39 FRR6 pKa = 11.84 RR7 pKa = 11.84 FRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 8.41 FKK13 pKa = 10.5 KK14 pKa = 9.62 FSRR17 pKa = 11.84 RR18 pKa = 11.84 FKK20 pKa = 10.22 RR21 pKa = 11.84 HH22 pKa = 5.26 FGGKK26 pKa = 8.2 RR27 pKa = 11.84 RR28 pKa = 11.84 KK29 pKa = 6.46 TTRR32 pKa = 11.84 QVQFKK37 pKa = 9.91 FKK39 pKa = 10.15 VQTVPYY45 pKa = 9.11 LNGSIAPSSSINWNNTSNTASHH67 pKa = 5.4 YY68 pKa = 9.69 TFAFTLGDD76 pKa = 3.45 IPHH79 pKa = 6.55 YY80 pKa = 10.65 SDD82 pKa = 4.11 LSSVFDD88 pKa = 3.9 AAKK91 pKa = 10.18 LAAVKK96 pKa = 10.46 LKK98 pKa = 9.77 FVPRR102 pKa = 11.84 YY103 pKa = 8.81 TMGQLPTSASTTYY116 pKa = 11.31 ANTSTPCVVVKK127 pKa = 10.53 DD128 pKa = 4.05 YY129 pKa = 11.81 DD130 pKa = 3.78 DD131 pKa = 5.57 ANPLTSYY138 pKa = 11.67 ANALLYY144 pKa = 10.79 QNARR148 pKa = 11.84 VVSILKK154 pKa = 9.83 PFSVYY159 pKa = 10.8 LKK161 pKa = 9.24 PKK163 pKa = 10.19 LSGGVEE169 pKa = 3.98 NTSLVIVAQSQARR182 pKa = 11.84 PWLDD186 pKa = 3.1 SGATAVPYY194 pKa = 10.56 YY195 pKa = 10.25 GVKK198 pKa = 10.64 LEE200 pKa = 4.12 VPGINTTQMLGQAIWDD216 pKa = 3.6 IYY218 pKa = 7.95 GTYY221 pKa = 9.42 YY222 pKa = 11.11 VKK224 pKa = 10.81 LKK226 pKa = 10.36 QIRR229 pKa = 11.84 LLL231 pKa = 3.93
Molecular weight: 26.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.279
IPC2_protein 10.028
IPC_protein 10.467
Toseland 10.511
ProMoST 10.175
Dawson 10.672
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 11.111
Grimsley 10.73
Solomon 10.701
Lehninger 10.672
Nozaki 10.467
DTASelect 10.335
Thurlkill 10.526
EMBOSS 10.891
Sillero 10.57
Patrickios 10.789
IPC_peptide 10.701
IPC2_peptide 9.107
IPC2.peptide.svr19 8.496
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
514
231
283
257.0
29.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.253 ± 1.516
1.167 ± 0.438
5.253 ± 1.068
4.28 ± 2.038
5.447 ± 0.108
6.809 ± 0.964
3.113 ± 1.083
4.475 ± 0.345
5.253 ± 1.774
7.977 ± 0.148
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.362 ± 0.038
3.502 ± 0.752
4.864 ± 0.198
4.669 ± 0.203
8.755 ± 1.092
6.809 ± 1.104
6.809 ± 0.845
6.031 ± 1.31
2.335 ± 0.618
5.837 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here