Molossus molossus circovirus 3 
Average proteome isoelectric point is 7.79 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A2I2MP65|A0A2I2MP65_9CIRC Capsid OS=Molossus molossus circovirus 3 OX=1959844 GN=Cap PE=4 SV=1MM1 pKa = 7.39  QSRR4 pKa = 11.84  HH5 pKa = 5.0  YY6 pKa = 10.78  CFTLNNPKK14 pKa = 10.28  EE15 pKa = 4.65  LIDD18 pKa = 3.88  FTTIPHH24 pKa = 5.06  VRR26 pKa = 11.84  YY27 pKa = 9.79  AIYY30 pKa = 10.12  QEE32 pKa = 4.66  EE33 pKa = 4.25  IGEE36 pKa = 4.24  SGNYY40 pKa = 8.94  HH41 pKa = 5.03  FQGYY45 pKa = 9.72  IEE47 pKa = 4.5  FDD49 pKa = 3.07  RR50 pKa = 11.84  PTRR53 pKa = 11.84  LGFLQRR59 pKa = 11.84  HH60 pKa = 5.7  LNGGHH65 pKa = 6.03  FEE67 pKa = 4.1  LRR69 pKa = 11.84  RR70 pKa = 11.84  GSRR73 pKa = 11.84  DD74 pKa = 3.0  EE75 pKa = 3.74  ARR77 pKa = 11.84  EE78 pKa = 3.81  YY79 pKa = 10.66  CRR81 pKa = 11.84  KK82 pKa = 9.48  LDD84 pKa = 3.64  SRR86 pKa = 11.84  IGEE89 pKa = 4.14  VFEE92 pKa = 5.08  YY93 pKa = 11.07  GDD95 pKa = 4.0  WDD97 pKa = 3.29  QGGQGVRR104 pKa = 11.84  TDD106 pKa = 4.04  FNALHH111 pKa = 6.07  EE112 pKa = 4.65  LVRR115 pKa = 11.84  QGTPTSEE122 pKa = 5.15  IMDD125 pKa = 3.51  QMPSMFYY132 pKa = 9.96  RR133 pKa = 11.84  YY134 pKa = 9.66  HH135 pKa = 7.65  SGIGKK140 pKa = 9.02  AQLLCVQDD148 pKa = 4.17  RR149 pKa = 11.84  DD150 pKa = 3.71  WPTKK154 pKa = 10.34  IIVLYY159 pKa = 9.97  GASGTGKK166 pKa = 10.2  SRR168 pKa = 11.84  WAHH171 pKa = 6.41  DD172 pKa = 3.72  YY173 pKa = 10.31  CHH175 pKa = 7.23  PSHH178 pKa = 6.51  QYY180 pKa = 8.55  WVSNTKK186 pKa = 9.54  WFDD189 pKa = 3.3  GYY191 pKa = 10.77  EE192 pKa = 4.01  FQPVVVLDD200 pKa = 4.77  DD201 pKa = 3.86  FRR203 pKa = 11.84  GWLPLHH209 pKa = 5.84  QLLRR213 pKa = 11.84  LCDD216 pKa = 3.96  RR217 pKa = 11.84  YY218 pKa = 10.41  PYY220 pKa = 8.73  TVEE223 pKa = 3.51  WKK225 pKa = 9.88  GGSRR229 pKa = 11.84  KK230 pKa = 8.16  FTAHH234 pKa = 6.34  VIIITSTRR242 pKa = 11.84  RR243 pKa = 11.84  PDD245 pKa = 2.7  EE246 pKa = 4.04  WYY248 pKa = 9.92  NWEE251 pKa = 4.53  EE252 pKa = 3.9  LHH254 pKa = 7.05  EE255 pKa = 5.37  DD256 pKa = 3.26  PAQLFRR262 pKa = 11.84  RR263 pKa = 11.84  IDD265 pKa = 3.5  EE266 pKa = 4.37  FLGRR270 pKa = 11.84  TPRR273 pKa = 11.84  RR274 pKa = 11.84  SRR276 pKa = 11.84  TALSEE281 pKa = 4.05  DD282 pKa = 3.48  RR283 pKa = 4.83  
 33.65 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  6.297 
IPC2_protein 6.224 
IPC_protein 6.364 
Toseland    6.693 
ProMoST     6.81 
Dawson      6.722 
Bjellqvist  6.678 
Wikipedia   6.751 
Rodwell     6.722 
Grimsley    6.839 
Solomon     6.737 
Lehninger   6.737 
Nozaki      6.985 
DTASelect   7.161 
Thurlkill   7.19 
EMBOSS      7.176 
Sillero     7.117 
Patrickios  4.075 
IPC_peptide 6.751 
IPC2_peptide  7.015 
IPC2.peptide.svr19  6.896 
 Protein with the highest isoelectric point: 
>tr|A0A2I2MP65|A0A2I2MP65_9CIRC Capsid OS=Molossus molossus circovirus 3 OX=1959844 GN=Cap PE=4 SV=1MM1 pKa = 7.36  RR2 pKa = 11.84  RR3 pKa = 11.84  KK4 pKa = 9.39  FRR6 pKa = 11.84  RR7 pKa = 11.84  FRR9 pKa = 11.84  RR10 pKa = 11.84  KK11 pKa = 8.41  FKK13 pKa = 10.5  KK14 pKa = 9.62  FSRR17 pKa = 11.84  RR18 pKa = 11.84  FKK20 pKa = 10.22  RR21 pKa = 11.84  HH22 pKa = 5.26  FGGKK26 pKa = 8.2  RR27 pKa = 11.84  RR28 pKa = 11.84  KK29 pKa = 6.46  TTRR32 pKa = 11.84  QVQFKK37 pKa = 9.91  FKK39 pKa = 10.15  VQTVPYY45 pKa = 9.11  LNGSIAPSSSINWNNTSNTASHH67 pKa = 5.4  YY68 pKa = 9.69  TFAFTLGDD76 pKa = 3.45  IPHH79 pKa = 6.55  YY80 pKa = 10.65  SDD82 pKa = 4.11  LSSVFDD88 pKa = 3.9  AAKK91 pKa = 10.18  LAAVKK96 pKa = 10.46  LKK98 pKa = 9.77  FVPRR102 pKa = 11.84  YY103 pKa = 8.81  TMGQLPTSASTTYY116 pKa = 11.31  ANTSTPCVVVKK127 pKa = 10.53  DD128 pKa = 4.05  YY129 pKa = 11.81  DD130 pKa = 3.78  DD131 pKa = 5.57  ANPLTSYY138 pKa = 11.67  ANALLYY144 pKa = 10.79  QNARR148 pKa = 11.84  VVSILKK154 pKa = 9.83  PFSVYY159 pKa = 10.8  LKK161 pKa = 9.24  PKK163 pKa = 10.19  LSGGVEE169 pKa = 3.98  NTSLVIVAQSQARR182 pKa = 11.84  PWLDD186 pKa = 3.1  SGATAVPYY194 pKa = 10.56  YY195 pKa = 10.25  GVKK198 pKa = 10.64  LEE200 pKa = 4.12  VPGINTTQMLGQAIWDD216 pKa = 3.6  IYY218 pKa = 7.95  GTYY221 pKa = 9.42  YY222 pKa = 11.11  VKK224 pKa = 10.81  LKK226 pKa = 10.36  QIRR229 pKa = 11.84  LLL231 pKa = 3.93  
 26.18 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.279 
IPC2_protein 10.028 
IPC_protein 10.467 
Toseland    10.511 
ProMoST     10.175 
Dawson      10.672 
Bjellqvist  10.35 
Wikipedia   10.847 
Rodwell     11.111 
Grimsley    10.73 
Solomon     10.701 
Lehninger   10.672 
Nozaki      10.467 
DTASelect   10.335 
Thurlkill   10.526 
EMBOSS      10.891 
Sillero     10.57 
Patrickios  10.789 
IPC_peptide 10.701 
IPC2_peptide  9.107 
IPC2.peptide.svr19  8.496 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2 
0
2 
514
231
283
257.0
29.91
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        5.253 ± 1.516
1.167 ± 0.438
5.253 ± 1.068
4.28 ± 2.038
5.447 ± 0.108
6.809 ± 0.964
3.113 ± 1.083
4.475 ± 0.345
5.253 ± 1.774
7.977 ± 0.148
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.362 ± 0.038
3.502 ± 0.752
4.864 ± 0.198
4.669 ± 0.203
8.755 ± 1.092
6.809 ± 1.104
6.809 ± 0.845
6.031 ± 1.31
2.335 ± 0.618
5.837 ± 0.134
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here