Rice stripe mosaic virus
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8D6S4|A0A1P8D6S4_9RHAB Nucleocapsid protein OS=Rice stripe mosaic virus OX=1931356 PE=4 SV=1
MM1 pKa = 7.46 SVPEE5 pKa = 4.09 DD6 pKa = 3.84 TPFRR10 pKa = 11.84 SYY12 pKa = 11.63 SSIFDD17 pKa = 4.04 DD18 pKa = 4.2 SDD20 pKa = 3.69 FVQPQPMSFKK30 pKa = 9.59 ATKK33 pKa = 9.8 EE34 pKa = 4.17 SEE36 pKa = 4.37 SLPEE40 pKa = 4.01 TEE42 pKa = 4.98 KK43 pKa = 11.28 EE44 pKa = 4.21 DD45 pKa = 3.38 MSTEE49 pKa = 4.05 YY50 pKa = 10.74 LSEE53 pKa = 4.07 PLRR56 pKa = 11.84 TKK58 pKa = 10.31 SGKK61 pKa = 9.53 KK62 pKa = 8.14 NRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 KK67 pKa = 10.45 GKK69 pKa = 9.21 DD70 pKa = 2.95 LKK72 pKa = 11.39 SLFTQEE78 pKa = 4.41 AGLPAPEE85 pKa = 5.01 ADD87 pKa = 3.37 SVLPEE92 pKa = 3.95 SSPYY96 pKa = 10.95 EE97 pKa = 3.95 NDD99 pKa = 3.15 NAQLEE104 pKa = 4.5 LPKK107 pKa = 10.41 PILKK111 pKa = 9.66 TSDD114 pKa = 3.1 APVFLRR120 pKa = 11.84 EE121 pKa = 4.01 KK122 pKa = 10.69 DD123 pKa = 3.5 LSKK126 pKa = 11.11 EE127 pKa = 3.48 FAAACKK133 pKa = 10.03 TNGILPRR140 pKa = 11.84 DD141 pKa = 3.53 EE142 pKa = 4.43 WKK144 pKa = 10.92 SSVAAKK150 pKa = 9.57 YY151 pKa = 9.65 HH152 pKa = 5.7 AEE154 pKa = 3.97 EE155 pKa = 4.43 GKK157 pKa = 7.84 MTKK160 pKa = 9.76 RR161 pKa = 11.84 DD162 pKa = 2.87 ISLIVFGMEE171 pKa = 3.9 LYY173 pKa = 10.37 KK174 pKa = 10.42 RR175 pKa = 11.84 YY176 pKa = 9.82 NVEE179 pKa = 4.27 SEE181 pKa = 4.03 VSTLFTSLVTEE192 pKa = 4.12 LQGIKK197 pKa = 10.12 VAAKK201 pKa = 9.15 EE202 pKa = 4.02 LNDD205 pKa = 3.5 TRR207 pKa = 11.84 EE208 pKa = 4.27 VLTKK212 pKa = 10.08 IPGEE216 pKa = 3.76 IVSAVKK222 pKa = 10.61 AGVKK226 pKa = 9.86 EE227 pKa = 4.11 GTEE230 pKa = 3.64 MGMDD234 pKa = 3.94 YY235 pKa = 10.82 IEE237 pKa = 4.6 TRR239 pKa = 11.84 TKK241 pKa = 10.38 VAPKK245 pKa = 9.91 SAPKK249 pKa = 10.32 VDD251 pKa = 3.08 ISKK254 pKa = 10.31 PMSSKK259 pKa = 10.28 MMEE262 pKa = 4.16 QQDD265 pKa = 3.77 EE266 pKa = 4.5 SSDD269 pKa = 3.5 EE270 pKa = 4.26 SSDD273 pKa = 3.77 NEE275 pKa = 4.19 SEE277 pKa = 4.14 EE278 pKa = 4.29 SEE280 pKa = 4.25 EE281 pKa = 4.3 EE282 pKa = 4.19 SFEE285 pKa = 4.02 TKK287 pKa = 10.39 AAIFLALIKK296 pKa = 10.73 VPEE299 pKa = 4.17 EE300 pKa = 3.8 EE301 pKa = 3.89 RR302 pKa = 11.84 DD303 pKa = 3.61 NAIVLMALRR312 pKa = 11.84 AVISDD317 pKa = 3.91 SEE319 pKa = 4.37 LNQAIRR325 pKa = 11.84 NDD327 pKa = 4.27 RR328 pKa = 11.84 ISSSVADD335 pKa = 4.2 MYY337 pKa = 10.43 HH338 pKa = 6.17 QKK340 pKa = 10.32 ISDD343 pKa = 3.7 KK344 pKa = 10.98 ARR346 pKa = 11.84 EE347 pKa = 3.98 LMGKK351 pKa = 10.29 GKK353 pKa = 8.22 TNKK356 pKa = 9.56 RR357 pKa = 11.84 AKK359 pKa = 9.6 QPKK362 pKa = 7.39 SSKK365 pKa = 10.03 YY366 pKa = 10.53 ASDD369 pKa = 4.44 YY370 pKa = 11.43 YY371 pKa = 11.44 DD372 pKa = 4.1 DD373 pKa = 4.87 ALL375 pKa = 5.77
Molecular weight: 41.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.067
IPC2_protein 5.041
IPC_protein 4.965
Toseland 4.825
ProMoST 5.054
Dawson 4.889
Bjellqvist 5.041
Wikipedia 4.736
Rodwell 4.813
Grimsley 4.736
Solomon 4.889
Lehninger 4.851
Nozaki 5.003
DTASelect 5.118
Thurlkill 4.813
EMBOSS 4.749
Sillero 5.08
Patrickios 4.457
IPC_peptide 4.902
IPC2_peptide 5.067
IPC2.peptide.svr19 5.115
Protein with the highest isoelectric point:
>tr|A0A1P8D6S6|A0A1P8D6S6_9RHAB Matrix protein OS=Rice stripe mosaic virus OX=1931356 PE=4 SV=1
MM1 pKa = 7.44 EE2 pKa = 5.86 FNWPWGQNSEE12 pKa = 4.37 TEE14 pKa = 3.71 ITKK17 pKa = 10.16 NLRR20 pKa = 11.84 FEE22 pKa = 5.33 DD23 pKa = 3.8 IKK25 pKa = 11.67 VMAIIILVWVKK36 pKa = 10.72 CLLIYY41 pKa = 10.29 HH42 pKa = 6.92 FKK44 pKa = 10.77 RR45 pKa = 11.84 KK46 pKa = 8.95 IRR48 pKa = 11.84 RR49 pKa = 11.84 LRR51 pKa = 11.84 SLLIKK56 pKa = 10.7 GSSQWVLHH64 pKa = 6.44 DD65 pKa = 3.73 AA66 pKa = 4.44
Molecular weight: 8.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.053
IPC2_protein 9.151
IPC_protein 9.18
Toseland 10.204
ProMoST 9.692
Dawson 10.292
Bjellqvist 9.897
Wikipedia 10.394
Rodwell 10.877
Grimsley 10.321
Solomon 10.35
Lehninger 10.335
Nozaki 10.189
DTASelect 9.882
Thurlkill 10.189
EMBOSS 10.57
Sillero 10.218
Patrickios 10.716
IPC_peptide 10.35
IPC2_peptide 8.463
IPC2.peptide.svr19 8.176
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3885
66
2066
555.0
62.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.255 ± 1.298
1.57 ± 0.459
5.663 ± 0.393
6.795 ± 0.905
3.604 ± 0.198
5.122 ± 0.471
1.673 ± 0.433
6.461 ± 0.738
7.027 ± 1.47
9.704 ± 1.001
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.192 ± 0.224
3.629 ± 0.132
4.633 ± 0.39
2.754 ± 0.169
5.431 ± 0.762
8.623 ± 0.84
6.435 ± 0.735
5.92 ± 0.467
1.493 ± 0.339
4.015 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here