Cerasibacillus quisquiliarum
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2778 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A511UZG6|A0A511UZG6_9BACI Uncharacterized protein OS=Cerasibacillus quisquiliarum OX=227865 GN=CQU01_22850 PE=4 SV=1
MM1 pKa = 7.49 PTFDD5 pKa = 3.44 QQNFFPVEE13 pKa = 4.1 KK14 pKa = 9.9 EE15 pKa = 3.58 LGMSFKK21 pKa = 10.09 PQKK24 pKa = 10.32 EE25 pKa = 4.2 LISFDD30 pKa = 4.06 DD31 pKa = 3.51 YY32 pKa = 11.61 RR33 pKa = 11.84 FNQITNLEE41 pKa = 4.1 NLSDD45 pKa = 4.15 DD46 pKa = 4.41 EE47 pKa = 4.48 YY48 pKa = 11.83 DD49 pKa = 4.47 EE50 pKa = 4.58 IAEE53 pKa = 4.4 SYY55 pKa = 10.79 IEE57 pKa = 4.07 LTTYY61 pKa = 11.05 SSGSKK66 pKa = 10.26 LSGYY70 pKa = 9.56 PVFTQDD76 pKa = 3.33 DD77 pKa = 3.81 PRR79 pKa = 11.84 YY80 pKa = 8.46 KK81 pKa = 10.25 EE82 pKa = 4.09 QYY84 pKa = 9.03 QSYY87 pKa = 10.55 DD88 pKa = 2.88 ILLFQIDD95 pKa = 4.18 SYY97 pKa = 10.52 DD98 pKa = 3.43 TPGIMWGDD106 pKa = 3.21 AGVANYY112 pKa = 9.64 FITSGDD118 pKa = 3.48 LKK120 pKa = 11.04 SRR122 pKa = 11.84 NLLNVLHH129 pKa = 7.33 DD130 pKa = 4.33 WDD132 pKa = 3.97 CHH134 pKa = 5.12
Molecular weight: 15.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 4.024
IPC_protein 3.999
Toseland 3.783
ProMoST 4.139
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.91
Rodwell 3.821
Grimsley 3.694
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.329
Thurlkill 3.834
EMBOSS 3.923
Sillero 4.113
Patrickios 1.074
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 4.002
Protein with the highest isoelectric point:
>tr|A0A511V1T1|A0A511V1T1_9BACI tRNA-dihydrouridine synthase OS=Cerasibacillus quisquiliarum OX=227865 GN=CQU01_15400 PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPHH8 pKa = 4.56 NRR10 pKa = 11.84 KK11 pKa = 9.3 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 VHH16 pKa = 6.06 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.49 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.45
IPC2_protein 11.169
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.427
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2778
0
2778
778283
39
2156
280.2
31.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.411 ± 0.049
0.626 ± 0.015
5.391 ± 0.041
7.377 ± 0.055
4.522 ± 0.042
6.4 ± 0.044
2.409 ± 0.028
8.656 ± 0.055
7.278 ± 0.044
9.661 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.86 ± 0.022
4.359 ± 0.036
3.504 ± 0.027
3.899 ± 0.035
4.163 ± 0.04
5.522 ± 0.037
5.612 ± 0.03
6.756 ± 0.04
0.933 ± 0.017
3.661 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here