Asticcacaulis excentricus (strain ATCC 15261 / DSM 4724 / VKM B-1370 / CB 48)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Asticcacaulis; Asticcacaulis excentricus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3743 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E8RP86|E8RP86_ASTEC Response regulator receiver protein OS=Asticcacaulis excentricus (strain ATCC 15261 / DSM 4724 / VKM B-1370 / CB 48) OX=573065 GN=Astex_0232 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84IDD4 pKa = 3.65VYY6 pKa = 11.48LSFNGQCAEE15 pKa = 4.07AFAFYY20 pKa = 10.61QSVLGGEE27 pKa = 4.54LMTFPFAAAPDD38 pKa = 4.08MPVDD42 pKa = 3.81PALKK46 pKa = 9.62DD47 pKa = 3.48WVMHH51 pKa = 5.68ATLNTGDD58 pKa = 4.99GIIQGADD65 pKa = 3.41CPPEE69 pKa = 4.07YY70 pKa = 11.01GPANPSGFCVSIQPPTVEE88 pKa = 3.82EE89 pKa = 4.16TQRR92 pKa = 11.84IWDD95 pKa = 4.36GLTQDD100 pKa = 3.59ARR102 pKa = 11.84AVNMPLAPTFWSPLFGMFIDD122 pKa = 5.47KK123 pKa = 10.69YY124 pKa = 8.77GQPWMINTVADD135 pKa = 3.95EE136 pKa = 4.65AFVAANTPQQ145 pKa = 2.92

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E8RRY7|E8RRY7_ASTEC Antibiotic biosynthesis monooxygenase OS=Asticcacaulis excentricus (strain ATCC 15261 / DSM 4724 / VKM B-1370 / CB 48) OX=573065 GN=Astex_1848 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.25NGQKK29 pKa = 9.79VVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3743

0

3743

1252122

30

2500

334.5

36.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.681 ± 0.051

0.79 ± 0.012

5.745 ± 0.031

5.458 ± 0.039

3.797 ± 0.024

8.205 ± 0.036

1.992 ± 0.024

4.887 ± 0.029

4.043 ± 0.03

10.138 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.019

2.981 ± 0.028

5.075 ± 0.03

3.446 ± 0.023

6.46 ± 0.04

5.827 ± 0.037

5.839 ± 0.045

7.209 ± 0.032

1.424 ± 0.018

2.709 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski