Thermoanaerobacterium sp. RBIITD

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacterales Family III. Incertae Sedis; Thermoanaerobacterium; unclassified Thermoanaerobacterium

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2986 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285BHH6|A0A285BHH6_9FIRM 50S ribosomal protein L11 OS=Thermoanaerobacterium sp. RBIITD OX=1550240 GN=rplK PE=3 SV=1
MM1 pKa = 7.9DD2 pKa = 3.56KK3 pKa = 10.57WEE5 pKa = 4.52CTVCGYY11 pKa = 10.3IYY13 pKa = 10.4DD14 pKa = 4.39PEE16 pKa = 5.2VGDD19 pKa = 3.77PTQNIAPGTRR29 pKa = 11.84FEE31 pKa = 5.38DD32 pKa = 5.09LPDD35 pKa = 3.76DD36 pKa = 4.45WVCPDD41 pKa = 3.9CGVGKK46 pKa = 9.66EE47 pKa = 4.02QFEE50 pKa = 4.79KK51 pKa = 10.63II52 pKa = 3.77

Molecular weight: 5.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285BF27|A0A285BF27_9FIRM Stage 0 sporulation protein A homolog OS=Thermoanaerobacterium sp. RBIITD OX=1550240 GN=SAMN05660242_1295 PE=4 SV=1
MM1 pKa = 7.5LRR3 pKa = 11.84THH5 pKa = 5.51QPKK8 pKa = 9.32VRR10 pKa = 11.84HH11 pKa = 6.04RR12 pKa = 11.84KK13 pKa = 8.01MEE15 pKa = 4.18HH16 pKa = 5.83GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 9.91NGRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.48GRR39 pKa = 11.84HH40 pKa = 5.56KK41 pKa = 10.21LTAA44 pKa = 4.1

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2986

0

2986

911818

36

2873

305.4

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.743 ± 0.046

0.977 ± 0.019

6.114 ± 0.04

6.548 ± 0.055

4.261 ± 0.038

6.474 ± 0.037

1.412 ± 0.019

10.236 ± 0.054

8.915 ± 0.045

8.977 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.021

6.247 ± 0.04

3.215 ± 0.025

2.321 ± 0.027

3.583 ± 0.029

5.85 ± 0.034

4.838 ± 0.038

6.42 ± 0.035

0.774 ± 0.016

4.451 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski