Extra small virus
Average proteome isoelectric point is 8.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8UX71|A8UX71_9VIRU Capsid protein OS=Extra small virus OX=230604 GN=cp17 PE=4 SV=1
MM1 pKa = 7.63 NKK3 pKa = 10.13 RR4 pKa = 11.84 INNNRR9 pKa = 11.84 RR10 pKa = 11.84 TMRR13 pKa = 11.84 SRR15 pKa = 11.84 RR16 pKa = 11.84 GRR18 pKa = 11.84 GRR20 pKa = 11.84 TMGSNLIPYY29 pKa = 9.25 ANSPVPIPYY38 pKa = 8.5 TPPVTPVTVIGNPRR52 pKa = 11.84 KK53 pKa = 6.74 TTWIDD58 pKa = 2.69 IDD60 pKa = 4.07 LSSEE64 pKa = 3.75 EE65 pKa = 4.13 SGIYY69 pKa = 9.22 TLTVGSYY76 pKa = 10.39 RR77 pKa = 11.84 NRR79 pKa = 11.84 ITKK82 pKa = 10.28 LGPSKK87 pKa = 10.75 PNFIIEE93 pKa = 4.1 KK94 pKa = 7.61 VAAYY98 pKa = 7.84 AAPGDD103 pKa = 4.03 YY104 pKa = 10.35 KK105 pKa = 11.37 VVLNDD110 pKa = 4.07 FKK112 pKa = 11.07 TGIQVVDD119 pKa = 3.81 EE120 pKa = 4.14 GSYY123 pKa = 10.19 AHH125 pKa = 7.35 RR126 pKa = 11.84 AAAGILYY133 pKa = 9.16 PPAAQIFYY141 pKa = 10.11 GISATGTLNTITTTAKK157 pKa = 10.72 DD158 pKa = 3.49 PVPVVRR164 pKa = 11.84 ALVTYY169 pKa = 9.59 WDD171 pKa = 4.03 SEE173 pKa = 4.24 QQ174 pKa = 3.47
Molecular weight: 19.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.793
IPC2_protein 9.575
IPC_protein 9.897
Toseland 9.853
ProMoST 9.721
Dawson 10.16
Bjellqvist 9.897
Wikipedia 10.394
Rodwell 10.35
Grimsley 10.262
Solomon 10.189
Lehninger 10.131
Nozaki 9.823
DTASelect 9.897
Thurlkill 9.97
EMBOSS 10.292
Sillero 10.058
Patrickios 5.283
IPC_peptide 10.175
IPC2_peptide 8.624
IPC2.peptide.svr19 8.545
Protein with the highest isoelectric point:
>tr|A8UX71|A8UX71_9VIRU Capsid protein OS=Extra small virus OX=230604 GN=cp17 PE=4 SV=1
MM1 pKa = 7.63 NKK3 pKa = 10.13 RR4 pKa = 11.84 INNNRR9 pKa = 11.84 RR10 pKa = 11.84 TMRR13 pKa = 11.84 SRR15 pKa = 11.84 RR16 pKa = 11.84 GRR18 pKa = 11.84 GRR20 pKa = 11.84 TMGSNLIPYY29 pKa = 9.25 ANSPVPIPYY38 pKa = 8.5 TPPVTPVTVIGNPRR52 pKa = 11.84 KK53 pKa = 6.74 TTWIDD58 pKa = 2.69 IDD60 pKa = 4.07 LSSEE64 pKa = 3.75 EE65 pKa = 4.13 SGIYY69 pKa = 9.22 TLTVGSYY76 pKa = 10.39 RR77 pKa = 11.84 NRR79 pKa = 11.84 ITKK82 pKa = 10.28 LGPSKK87 pKa = 10.75 PNFIIEE93 pKa = 4.1 KK94 pKa = 7.61 VAAYY98 pKa = 7.84 AAPGDD103 pKa = 4.03 YY104 pKa = 10.35 KK105 pKa = 11.37 VVLNDD110 pKa = 4.07 FKK112 pKa = 11.07 TGIQVVDD119 pKa = 3.81 EE120 pKa = 4.14 GSYY123 pKa = 10.19 AHH125 pKa = 7.35 RR126 pKa = 11.84 AAAGILYY133 pKa = 9.16 PPAAQIFYY141 pKa = 10.11 GISATGTLNTITTTAKK157 pKa = 10.72 DD158 pKa = 3.49 PVPVVRR164 pKa = 11.84 ALVTYY169 pKa = 9.59 WDD171 pKa = 4.03 SEE173 pKa = 4.24 QQ174 pKa = 3.47
Molecular weight: 19.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.793
IPC2_protein 9.575
IPC_protein 9.897
Toseland 9.853
ProMoST 9.721
Dawson 10.16
Bjellqvist 9.897
Wikipedia 10.394
Rodwell 10.35
Grimsley 10.262
Solomon 10.189
Lehninger 10.131
Nozaki 9.823
DTASelect 9.897
Thurlkill 9.97
EMBOSS 10.292
Sillero 10.058
Patrickios 5.283
IPC_peptide 10.175
IPC2_peptide 8.624
IPC2.peptide.svr19 8.545
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
174
174
174
174.0
19.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.046 ± 0.0
0.0 ± 0.0
4.023 ± 0.0
2.874 ± 0.0
1.724 ± 0.0
7.471 ± 0.0
0.575 ± 0.0
8.621 ± 0.0
4.598 ± 0.0
4.598 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.724 ± 0.0
6.322 ± 0.0
8.621 ± 0.0
1.724 ± 0.0
7.471 ± 0.0
6.322 ± 0.0
10.345 ± 0.0
8.046 ± 0.0
1.149 ± 0.0
5.747 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here