Streptococcus satellite phage Javan76

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZW83|A0A4D5ZW83_9VIRU Clp protease-like protein OS=Streptococcus satellite phage Javan76 OX=2558855 GN=JavanS76_0012 PE=3 SV=1
MM1 pKa = 7.22NRR3 pKa = 11.84IKK5 pKa = 10.53EE6 pKa = 4.04LRR8 pKa = 11.84QKK10 pKa = 10.88EE11 pKa = 4.4KK12 pKa = 10.13LTQEE16 pKa = 3.8EE17 pKa = 4.69LANKK21 pKa = 9.97IGVTKK26 pKa = 9.8RR27 pKa = 11.84TIIAWEE33 pKa = 3.85KK34 pKa = 10.72GEE36 pKa = 4.18RR37 pKa = 11.84QIKK40 pKa = 8.54QDD42 pKa = 3.7KK43 pKa = 9.29AQQLADD49 pKa = 3.63YY50 pKa = 9.54FGVSVRR56 pKa = 11.84YY57 pKa = 9.53LLGYY61 pKa = 9.87EE62 pKa = 4.35DD63 pKa = 5.98DD64 pKa = 4.68YY65 pKa = 11.27LSSMIKK71 pKa = 10.12KK72 pKa = 10.21YY73 pKa = 10.37EE74 pKa = 3.85EE75 pKa = 4.29SMKK78 pKa = 10.92NPADD82 pKa = 3.73FGGYY86 pKa = 10.12GLLALTRR93 pKa = 11.84GTEE96 pKa = 4.09VQDD99 pKa = 3.75KK100 pKa = 10.5VIEE103 pKa = 4.23ALRR106 pKa = 11.84HH107 pKa = 4.39ATDD110 pKa = 3.93YY111 pKa = 10.96YY112 pKa = 11.14GEE114 pKa = 4.17RR115 pKa = 11.84ALNRR119 pKa = 11.84KK120 pKa = 7.46EE121 pKa = 3.81AEE123 pKa = 3.8KK124 pKa = 10.06WDD126 pKa = 3.6EE127 pKa = 3.84EE128 pKa = 4.03RR129 pKa = 11.84RR130 pKa = 11.84AEE132 pKa = 4.06ILEE135 pKa = 4.08SLKK138 pKa = 10.87DD139 pKa = 3.64YY140 pKa = 10.91SDD142 pKa = 4.45NIIGRR147 pKa = 11.84FLAGLMSLSDD157 pKa = 3.7EE158 pKa = 4.26LKK160 pKa = 10.43IPIVDD165 pKa = 4.83FLTLEE170 pKa = 4.81DD171 pKa = 3.87KK172 pKa = 11.17DD173 pKa = 3.86KK174 pKa = 11.44AAVSQIIISLADD186 pKa = 3.54NPVMHH191 pKa = 7.38KK192 pKa = 10.48DD193 pKa = 3.45YY194 pKa = 11.26SSQQKK199 pKa = 5.97TQKK202 pKa = 11.09DD203 pKa = 3.51NN204 pKa = 3.55

Molecular weight: 23.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZWX0|A0A4D5ZWX0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan76 OX=2558855 GN=JavanS76_0011 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 9.33ITEE5 pKa = 4.22VKK7 pKa = 9.97KK8 pKa = 10.98KK9 pKa = 10.66NGTTVYY15 pKa = 10.11RR16 pKa = 11.84ASVYY20 pKa = 10.76LGVDD24 pKa = 2.9QVTGKK29 pKa = 10.16KK30 pKa = 10.27VKK32 pKa = 9.74TSVTGRR38 pKa = 11.84TRR40 pKa = 11.84KK41 pKa = 8.7EE42 pKa = 3.85VKK44 pKa = 10.32AKK46 pKa = 10.36AQQAQIDD53 pKa = 4.14FKK55 pKa = 11.72LNGSTVKK62 pKa = 10.73KK63 pKa = 9.64EE64 pKa = 4.03MQVRR68 pKa = 11.84TYY70 pKa = 11.03QEE72 pKa = 4.99LADD75 pKa = 3.71LWLRR79 pKa = 11.84SYY81 pKa = 11.31QLTVKK86 pKa = 9.75PQTFRR91 pKa = 11.84ATQRR95 pKa = 11.84MLYY98 pKa = 9.75NHH100 pKa = 7.94LIPAFGTMKK109 pKa = 10.09PNKK112 pKa = 7.9ITVYY116 pKa = 10.29YY117 pKa = 9.73IQDD120 pKa = 4.67FINDD124 pKa = 3.66LSKK127 pKa = 11.04EE128 pKa = 4.12LVHH131 pKa = 6.35YY132 pKa = 10.53SVVHH136 pKa = 5.68SINRR140 pKa = 11.84RR141 pKa = 11.84VLQYY145 pKa = 10.88GVSLQLLPFNPARR158 pKa = 11.84DD159 pKa = 3.75VILPKK164 pKa = 10.34VPKK167 pKa = 10.22RR168 pKa = 11.84EE169 pKa = 3.82NKK171 pKa = 9.52VIKK174 pKa = 10.34FIAPEE179 pKa = 4.17NIKK182 pKa = 11.09ALMDD186 pKa = 3.7YY187 pKa = 10.18MEE189 pKa = 4.85SLSNQKK195 pKa = 9.91YY196 pKa = 10.27GYY198 pKa = 9.07YY199 pKa = 9.96LDD201 pKa = 3.88YY202 pKa = 11.22VLYY205 pKa = 10.56SVLLATGCRR214 pKa = 11.84FGEE217 pKa = 4.58VVALEE222 pKa = 4.21WSDD225 pKa = 3.5IDD227 pKa = 5.03LKK229 pKa = 11.17EE230 pKa = 3.96KK231 pKa = 10.12TISITKK237 pKa = 9.92NYY239 pKa = 9.67NRR241 pKa = 11.84LLKK244 pKa = 10.94LIGTPKK250 pKa = 9.91SKK252 pKa = 10.87AGVRR256 pKa = 11.84VISIDD261 pKa = 3.32KK262 pKa = 9.22KK263 pKa = 7.66TVNMLRR269 pKa = 11.84LYY271 pKa = 10.81KK272 pKa = 10.3NRR274 pKa = 11.84QRR276 pKa = 11.84QLFLEE281 pKa = 4.56VDD283 pKa = 3.82SQVPTVVFATPTKK296 pKa = 9.74EE297 pKa = 4.11YY298 pKa = 10.79QNMATRR304 pKa = 11.84QEE306 pKa = 4.23SLDD309 pKa = 3.67RR310 pKa = 11.84RR311 pKa = 11.84LKK313 pKa = 10.47EE314 pKa = 3.81INCPRR319 pKa = 11.84FTFHH323 pKa = 7.88AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.49RR346 pKa = 11.84LGHH349 pKa = 6.03ATLATTMDD357 pKa = 3.91IYY359 pKa = 11.76SHH361 pKa = 6.95LSKK364 pKa = 10.92DD365 pKa = 3.51KK366 pKa = 10.41EE367 pKa = 4.25KK368 pKa = 10.93EE369 pKa = 3.85AVSYY373 pKa = 9.56YY374 pKa = 10.73EE375 pKa = 4.17KK376 pKa = 10.75AINNLL381 pKa = 3.4

Molecular weight: 44.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2527

52

525

194.4

22.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.698 ± 0.471

0.554 ± 0.189

5.421 ± 0.478

7.717 ± 0.676

4.195 ± 0.436

4.393 ± 0.474

1.82 ± 0.266

7.242 ± 0.518

10.645 ± 0.347

11.001 ± 0.524

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.41

5.54 ± 0.608

3.008 ± 0.35

3.997 ± 0.339

4.709 ± 0.543

5.184 ± 0.312

6.055 ± 0.544

4.986 ± 0.695

0.95 ± 0.188

4.63 ± 0.369
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski